pGPS5: Difference between revisions

Latest revision as of 18:37, 17 June 2010

At a Glance

Type	Donor
Origin of Replication	R6K
Copy #	High
Markers	Tetracycline, Kanamycin
Link to Sequence	NEB
Features	Tn7R ^[1] Tn7L Transprimer-5
Accession Number
edit table

Notes

pGPS5 will not replicate in ordinary lab strains of E. coli. Its origin of replication is from the transmissible plasmid R6K, and it depends on a replication initiation protein of plasmid origin (the protein product of the pir gene) not normally present in laboratory strains.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

New England Biolabs

Sequence

      1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 
     51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 
    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 
    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 
    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 
    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 
    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 
    351  GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT 
    401  AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA 
    451  CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC 
    501  ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA 
    551  GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG 
    601  TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA 
    651  CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA 
    701  AAGAGGTAAT GAAATGGCAT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT 
    751  ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG AGATGGCGGA 
    801  CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC 
    851  GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG 
    901  TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA 
    951  CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC 
   1001  CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA 
   1051  GCGGTCCAAT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA 
   1101  AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA 
   1151  CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG 
   1201  CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG 
   1251  GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC 
   1301  GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG 
   1351  CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG 
   1401  AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA 
   1451  GAAGACAGTC ATAAGTGCGG CGACAGTAGT CATGCCCCGC GCCCACCGGA 
   1501  AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC 
   1551  TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT 
   1601  GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA 
   1651  GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC 
   1701  ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 
   1751  ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC 
   1801  GTCCGGCGTA GAGGATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG 
   1851  TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA 
   1901  GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG 
   1951  CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG 
   2001  ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC 
   2051  TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG 
   2101  ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA 
   2151  CCGCATTAAA GCTTATCGAT GATAAGCGCT GGCATTGACC CTGAGTGATT 
   2201  TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG 
   2251  TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC
   2301  TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
   2351  ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG 
   2401  CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG 
   2451  ACGCGGATGA ACAGGCAGAG CTCTTACTGT CATGCCATCC GTATGTTTAA 
   2501  ACACAATAAA GTCTTAAACT GAACAAAATA GATCTAAACT ATGACAATAA 
   2551  AGTCTTAAAC TAGACAGAAT AGTTGTAAAC TGAAATCAGT CCAGTTATGC 
   2601  TGTGAAAAAG CATACTGGAC TTTTGTTATG GCTAAAGCAA ACTCTTCATT 
   2651  TTCTGAAGTG CAAATTGCCC GTCGTATTAA AGAGGGGCGT GGGGTCGACG 
   2701  CGGCCGCTAA CTATAACGGT CCTAAGGTAG CGAGTTTGGC GCGCCAAACG 
   2751  ATATCGGATC CGGCCGCCGC TGAGGTCTGC CTCGTGAAGA AGGTGTTGCT 
   2801  GACTCATACC AGGCCTGAAT CGCCCCATCA TCCAGCCAGA AAGTGAGGGA 
   2851  GCCACGGTTG ATGAGAGCTT TGTTGTAGGT GGACCAGTTG GTGATTTTGA 
   2901  ACTTTTGCTT TGCCACGGAA CGGTCTGCGT TGTCGGGAAG ATGCGTGATC 
   2951  TGATCCTTCA ACTCAGCAAG AGTTCGATTT ATTCAACAAA GCCGCCGTCC 
   3001  CGTCAAGTCA GCGTAATGCT CTGCCAGTGT TACAACCAAT TAACCAATTC 
   3051  TGATTAGAAA AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT 
   3101  CAGGATTATC AATACCATAT TTTTGAAAAA GCCGTTTCTG TAATGAAGGA 
   3151  GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT GGTATCGGTC 
   3201  TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT 
   3251  CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC 
   3301  GGTGAGAATG GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG 
   3351  CCAGCCATTA CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT 
   3401  TCATTCGTGA TTGCGCCTGA GCGAGACGAA ATACGCGATC GCTGTTAAAA 
   3451  GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA ACACTGCCAG 
   3501  CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA 
   3551  ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA 
   3601  GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA 
   3651  GTTTAGTCTG ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC 
   3701  CATGTTTCAG AAACAACTCT GGCGCATCGG GCTTCCCATA CAATCGATAG 
   3751  ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT TATACCCATA 
   3801  TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAG CAAGACGTTT 
   3851  CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA 
   3901  GACAGTTTTA TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA 
   3951  TCAGAGATTT TGAGACACAA CGTGGCTTAC TAGGATCCGA TATCATTTAA 
   4001  ATCTAGGGAT AACAGGGTAA TACTAGTGTC GACCAACCAG ATAAGTGAAA 
   4051  TCTAGTTCCA AACTATTTTG TCATTTTTAA TTTTCGTATT AGCTTACGAC 
   4101  GCTACACCCA GTTCCCATCT ATTTTGTCAC TCTTCCCTAA ATAATCCTTA 
   4151  AAAACTCCAT TTCCACCCCT CCCAGTTCCC AACTTTATTG TGTTTAAACA 
   4201  CCGTAAGATG CTTTTCAGTG ACT

References

^[2] ^[3] ^[4] ^[5] ^[6]

↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
↑ Stellwagen, AE & Craig, NL (1997) Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145 573-85 PubMed
↑ Biery, MC et al. (2000) A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucleic Acids Res. 28 1067-77 PubMed
↑ Metcalf, WW et al. (1994) Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138 1-7 PubMed
↑ Kolter, R et al. (1978) Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15 1199-208 PubMed

[PMID:8556868-1] Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed

[PMID:_8556868-2] Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed

[PMID:_9055068-3] Stellwagen, AE & Craig, NL (1997) Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145 573-85 PubMed

[PMID:_10666445-4] Biery, MC et al. (2000) A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucleic Acids Res. 28 1067-77 PubMed

[PMID:_8125283-5] Metcalf, WW et al. (1994) Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138 1-7 PubMed

[PMID:_728998-6] Kolter, R et al. (1978) Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15 1199-208 PubMed

[1]

[2]

[3]

[4]

[5]

[6]

@@ Line 1: / Line 1: @@
-==At a Glance {{HelpIcon|Help:Plasmid}}==
+==At a Glance==
+[[Image:pGPS5.jpg|thumb|400px|right|pGPS5, Image provided by [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pGPS4and5_map.pdf NEB]]]
 <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.X46dc5f507447a-->
 <!--
 ******************************************************************************************
 *
 *   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 *
 ****************************************************************************************** -->
-{|{{Prettytable}} width='30%' align=left
+{| {{Prettytable}} width='30%' align=left  id="X46dc5f507447a"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
+||
+Donor
 |-
-!{{table heading style}} |Origin
+!align=left {{table heading style}} |Origin of Replication
+||
+R6K
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Copy #
+||
+High
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Markers
+||
+Tetracycline, Kanamycin
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Link to Sequence
+||
+[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pgps5.txt NEB]
 |-
-!{{table heading style}} |Comments
+!align=left {{table heading style}} |Features
+||
+Tn7R <ref name='PMID: 8556868'/><br>
+Tn7L<br>
+Transprimer-5<br>
 |-
-!{{table heading style}} |References
+!align=left {{table heading style}} |Accession Number
-|-
+||
-|colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X46dc5f507447a&page=0&pagename={{FULLPAGENAMEE}} edit table]
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X46dc5f507447a&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.X46dc5f507447a--></protect>
 <BR>
 <Br clear='all'>
+==Notes==
+pGPS5 will not replicate in ordinary lab strains of E. coli. Its origin of replication is from the transmissible plasmid R6K, and it depends on a replication initiation protein of plasmid origin (the protein product of the pir gene) not normally present in laboratory strains.
 ==History {{HelpIcon|Help:Plasmid}}==
 {{PlasmidHistory}}
 ==Sources==
-pGPS5 is isolated from E. coli BW23474, an E. coli strain that expresses the pir-116 replication factor (4), by a standard plasmid purification procedure.
+[http://www.neb.com/nebecomm/products/productN7150.asp New England Biolabs]
 ==Sequence==
   GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
   TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC
   CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
   GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
   CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC
   ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
   CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA
   GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT
   AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA
   CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC
   ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA
   GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG
   TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA
   CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA
   AAGAGGTAAT GAAATGGCAT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT
   ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG AGATGGCGGA
   CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC
   GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG
   TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA
   CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC
   CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA
   GCGGTCCAAT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA
   AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA
   CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG
   CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG
   GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC
   GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG
   CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG
   AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA
   GAAGACAGTC ATAAGTGCGG CGACAGTAGT CATGCCCCGC GCCCACCGGA
   AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC
   TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT
   GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA
   GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC
   ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
   ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC
   GTCCGGCGTA GAGGATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG
   TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA
   GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG
   CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG
   ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC
   TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG
   ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA
   CCGCATTAAA GCTTATCGAT GATAAGCGCT GGCATTGACC CTGAGTGATT
   TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG
+  TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC
-  TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC
+  TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
-  TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
   ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG
   CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG
   ACGCGGATGA ACAGGCAGAG CTCTTACTGT CATGCCATCC GTATGTTTAA
   ACACAATAAA GTCTTAAACT GAACAAAATA GATCTAAACT ATGACAATAA
   AGTCTTAAAC TAGACAGAAT AGTTGTAAAC TGAAATCAGT CCAGTTATGC
   TGTGAAAAAG CATACTGGAC TTTTGTTATG GCTAAAGCAA ACTCTTCATT
   TTCTGAAGTG CAAATTGCCC GTCGTATTAA AGAGGGGCGT GGGGTCGACG
   CGGCCGCTAA CTATAACGGT CCTAAGGTAG CGAGTTTGGC GCGCCAAACG
   ATATCGGATC CGGCCGCCGC TGAGGTCTGC CTCGTGAAGA AGGTGTTGCT
   GACTCATACC AGGCCTGAAT CGCCCCATCA TCCAGCCAGA AAGTGAGGGA
   GCCACGGTTG ATGAGAGCTT TGTTGTAGGT GGACCAGTTG GTGATTTTGA
   ACTTTTGCTT TGCCACGGAA CGGTCTGCGT TGTCGGGAAG ATGCGTGATC
   TGATCCTTCA ACTCAGCAAG AGTTCGATTT ATTCAACAAA GCCGCCGTCC
   CGTCAAGTCA GCGTAATGCT CTGCCAGTGT TACAACCAAT TAACCAATTC
   TGATTAGAAA AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT
   CAGGATTATC AATACCATAT TTTTGAAAAA GCCGTTTCTG TAATGAAGGA
   GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT GGTATCGGTC
   TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT
   CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC
   GGTGAGAATG GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG
   CCAGCCATTA CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT
   TCATTCGTGA TTGCGCCTGA GCGAGACGAA ATACGCGATC GCTGTTAAAA
   GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA ACACTGCCAG
   CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA
   ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA
   GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA
   GTTTAGTCTG ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC
   CATGTTTCAG AAACAACTCT GGCGCATCGG GCTTCCCATA CAATCGATAG
   ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT TATACCCATA
   TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAG CAAGACGTTT
   CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA
   GACAGTTTTA TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA
   TCAGAGATTT TGAGACACAA CGTGGCTTAC TAGGATCCGA TATCATTTAA
   ATCTAGGGAT AACAGGGTAA TACTAGTGTC GACCAACCAG ATAAGTGAAA
   TCTAGTTCCA AACTATTTTG TCATTTTTAA TTTTCGTATT AGCTTACGAC
   GCTACACCCA GTTCCCATCT ATTTTGTCAC TCTTCCCTAA ATAATCCTTA
   AAAACTCCAT TTCCACCCCT CCCAGTTCCC AACTTTATTG TGTTTAAACA
   CCGTAAGATG CTTTTCAGTG ACT
-==Images {{HelpIcon|Help:Plasmid}}==
-{{PlasmidImage}}
 ==References==
-{{RefHelp}}
+<ref name='PMID: 8556868'/>
+<ref name='PMID: 9055068'/>
+<ref name='PMID: 10666445'/>
+<ref name='PMID: 8125283'/>
+<ref name='PMID: 728998'/>
 <references/>
 __NOTOC__
-[[Category:Plasmids]]
+[[Category:Plasmids]][[Category:Donor Plasmids]][[Category:NEB Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:Tetracycline Marker Plasmids]]