pGPS5
At a Glance
pGPS5, Image provided by NEB
<protect>
| Type |
Donor |
|---|---|
| Origin of Replication |
R6K |
| Copy # |
High |
| Markers |
Tetracycline, Kanamycin |
| Link to Sequence | |
| Features |
Tn7R [1] |
| Accession Number |
|
| edit table |
</protect>
Notes
pGPS5 will not replicate in ordinary lab strains of E. coli. Its origin of replication is from the transmissible plasmid R6K, and it depends on a replication initiation protein of plasmid origin (the protein product of the pir gene) not normally present in laboratory strains.
History
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Sources
Sequence
1 GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
51 TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC
101 CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
151 GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
201 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC
251 ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
301 CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA
351 GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT
401 AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA
451 CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC
501 ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA
551 GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG
601 TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA
651 CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA
701 AAGAGGTAAT GAAATGGCAT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT
751 ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG AGATGGCGGA
801 CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC
851 GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG
901 TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA
951 CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC
1001 CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA
1051 GCGGTCCAAT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA
1101 AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA
1151 CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG
1201 CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG
1251 GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC
1301 GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG
1351 CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG
1401 AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA
1451 GAAGACAGTC ATAAGTGCGG CGACAGTAGT CATGCCCCGC GCCCACCGGA
1501 AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC
1551 TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT
1601 GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA
1651 GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC
1701 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
1751 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC
1801 GTCCGGCGTA GAGGATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG
1851 TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA
1901 GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG
1951 CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG
2001 ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC
2051 TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG
2101 ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA
2151 CCGCATTAAA GCTTATCGAT GATAAGCGCT GGCATTGACC CTGAGTGATT
2201 TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG
2251 TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC
2301 TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
2351 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG
2401 CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG
2451 ACGCGGATGA ACAGGCAGAG CTCTTACTGT CATGCCATCC GTATGTTTAA
2501 ACACAATAAA GTCTTAAACT GAACAAAATA GATCTAAACT ATGACAATAA
2551 AGTCTTAAAC TAGACAGAAT AGTTGTAAAC TGAAATCAGT CCAGTTATGC
2601 TGTGAAAAAG CATACTGGAC TTTTGTTATG GCTAAAGCAA ACTCTTCATT
2651 TTCTGAAGTG CAAATTGCCC GTCGTATTAA AGAGGGGCGT GGGGTCGACG
2701 CGGCCGCTAA CTATAACGGT CCTAAGGTAG CGAGTTTGGC GCGCCAAACG
2751 ATATCGGATC CGGCCGCCGC TGAGGTCTGC CTCGTGAAGA AGGTGTTGCT
2801 GACTCATACC AGGCCTGAAT CGCCCCATCA TCCAGCCAGA AAGTGAGGGA
2851 GCCACGGTTG ATGAGAGCTT TGTTGTAGGT GGACCAGTTG GTGATTTTGA
2901 ACTTTTGCTT TGCCACGGAA CGGTCTGCGT TGTCGGGAAG ATGCGTGATC
2951 TGATCCTTCA ACTCAGCAAG AGTTCGATTT ATTCAACAAA GCCGCCGTCC
3001 CGTCAAGTCA GCGTAATGCT CTGCCAGTGT TACAACCAAT TAACCAATTC
3051 TGATTAGAAA AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT
3101 CAGGATTATC AATACCATAT TTTTGAAAAA GCCGTTTCTG TAATGAAGGA
3151 GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT GGTATCGGTC
3201 TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT
3251 CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC
3301 GGTGAGAATG GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG
3351 CCAGCCATTA CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT
3401 TCATTCGTGA TTGCGCCTGA GCGAGACGAA ATACGCGATC GCTGTTAAAA
3451 GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA ACACTGCCAG
3501 CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA
3551 ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA
3601 GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA
3651 GTTTAGTCTG ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC
3701 CATGTTTCAG AAACAACTCT GGCGCATCGG GCTTCCCATA CAATCGATAG
3751 ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT TATACCCATA
3801 TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAG CAAGACGTTT
3851 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA
3901 GACAGTTTTA TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA
3951 TCAGAGATTT TGAGACACAA CGTGGCTTAC TAGGATCCGA TATCATTTAA
4001 ATCTAGGGAT AACAGGGTAA TACTAGTGTC GACCAACCAG ATAAGTGAAA
4051 TCTAGTTCCA AACTATTTTG TCATTTTTAA TTTTCGTATT AGCTTACGAC
4101 GCTACACCCA GTTCCCATCT ATTTTGTCAC TCTTCCCTAA ATAATCCTTA
4151 AAAACTCCAT TTCCACCCCT CCCAGTTCCC AACTTTATTG TGTTTAAACA
4201 CCGTAAGATG CTTTTCAGTG ACT
References
- ↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
- ↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
- ↑ Stellwagen, AE & Craig, NL (1997) Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145 573-85 PubMed
- ↑ Biery, MC et al. (2000) A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucleic Acids Res. 28 1067-77 PubMed
- ↑ Metcalf, WW et al. (1994) Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138 1-7 PubMed
- ↑ Kolter, R et al. (1978) Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15 1199-208 PubMed
