pGPS1.1: Difference between revisions

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==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance==
[[image:PGPS1.1.jpg|thumb|400px|right| pGPS1.1, Image provided by [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pGPS11and21_map.pdf NEB]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="I46d7fd4fb118c"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
||
Transposon Donor
|-
|-
!{{table heading style}} |Origin
!align=left {{table heading style}} |Origin of Replication
||
R6K-γ<ref name='PMID:375227'/>
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
||
High
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
||
Tetracycline<br>
Kanamycin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pgps1.1.txt NEB]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
||
Transprimer-1<br>
Tn7R<br>
Tn7L
|-
|-
!{{table heading style}} |References
!align=left {{table heading style}} |Accession Number
|-
||
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==Notes==
pGPS1.1 is used as a trasposon donor in the GPS-1 Genome Priming System offered by NEB.  TnsABC transposase removes the [[Transposon Tn7|Tn7]]-based Transprimer element from pGPS1.1 and inserts it randomly into a trarget DNA molecule in vitro.  pGPS1.1 is isolated from E. coli BW 23474, a strain that expresses the pir-116 replication factor (4).
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Sources==
 
[http://www.neb.com/nebecomm/products/productN7111.asp New England Biolabs]


pGPS1.1 is isolated from E. coli BW23474, an E. coli strain that expresses the pir-116 replication factor (4), by a standard plasmid purification procedure.
==Sequence==
      1 GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
      51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC
    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC
    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA
    351  GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT
    401  AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA
    451  CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC
    501  ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA
    551  GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG
    601  TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA
    651  CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA
    701  AAGAGGTAAT GAAATGGCAG ATCCCTGGCT TGTTGTCCAC AACCGTTAAA
    751  CCTTAAAAGC TTTAAAAGCC TTATATATTC TTTTTTTTCT TATAAAACTT
    801  AAAACCTTAG AGGCTATTTA AGTTGCTGAT TTATATTAAT TTTATTGTTC
    851  AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTTAGCCAT
    901  GAGAGCTTAG TACGTTAGCC ATGAGGGTTT AGTTCGTTAA ACATGAGAGC
    951  TTAGTACGTT AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA
    1001  CGTACTATCA ACAGGTTGAA CTGCTGATCT TCGGATCTAT GTCGGGTGCG
    1051  GAGAAAGAGG TAATGAAATG GCATCCGGAT CTGCATCGCA GGATGCTGCT
    1101  GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCA TTATTGAAGC
    1151  ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
    1201  GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
    1251  CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
    1301  CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTCTCATGTT TGACAGCTTA
    1351  TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC
    1401  AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
    1451  CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC
    1501  CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT
    1551  GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT
    1601  TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT
    1651  CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC
    1701  CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC
    1751  AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC
    1801  GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG
    1851  GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC
    1901  ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT
    1951  TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA
    2001  GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
    2051  CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC
    2101  CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG
    2151  ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC
    2201  TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG
    2251  CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG
    2301  TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC
    2351  CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG
    2401  ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC
    2451  CTAACTTCGA TCATTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC
    2501  GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC
    2551  TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG
    2601  ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA
    2651  ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC
    2701  TTGGCAGAAC ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC
    2751  GCATCTCGGG CAGCGTTGGG TCCTGGGCTG GCATTGACCC TGAGTGATTT
    2801  TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT
    2851  TCCAGTAACC GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT
    2901  CTCTCGTTTC ATCGGTATCA TTACCCCCAT GAACAGAAAT CCCCCTTACA
    2951  CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA CATGGCCCGC
    3001  TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA
    3051  CGCGGATGAA CAGGCAGAGC TCTTACTGTC ATGCCATCCG TATGTGGGCG
    3101  GACAATAAAG TCTTAAACTG AACAAAATAG ATCTAAACTA TGACAATAAA
    3151  GTCTTAAACT AGACAGAATA GTTGTAAACT GAAATCAGTC CAGTTATGCT
    3201  GTGAAAAAGC ATACTGGACT TTTGTTATGG CTAAAGCAAA CTCTTCATTT
    3251  TCTGAAGTGC AAATTGCCCG TCGTATTAAA GAGGGGCGTG GGGTCGACGC
    3301  GGCCGCTAAC TATAACGGTC CTAAGGTAGC GAGTTTAAAC GATATCGGAT
    3351  CCGGCCGCCG CTGAGGTCTG CCTCGTGAAG AAGGTGTTGC TGACTCATAC
    3401  CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT
    3451  GATGAGAGCT TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT
    3501  TTGCCACGGA ACGGTCTGCG TTGTCGGGAA GATGCGTGAT CTGATCCTTC
    3551  AACTCAGCAA GAGTTCGATT TATTCAACAA AGCCGCCGTC CCGTCAAGTC
    3601  AGCGTAATGC TCTGCCAGTG TTACAACCAA TTAACCAATT CTGATTAGAA
    3651  AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT
    3701  CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA
    3751  CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC
    3801  GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA
    3851  GGTTATCAAG TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT
    3901  GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT
    3951  ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG
    4001  ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA
    4051  CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC
    4101  AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT
    4151  TCCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA
    4201  AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT
    4251  GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA
    4301  GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA GATTGTCGCA
    4351  CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC
    4401  ATCCATGTTG GAATTTAATC GCGGCCTCGA GCAAGACGTT TCCCGTTGAA
    4451  TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT
    4501  ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT
    4551  TTGAGACACA ACGTGGCTTA CTAGGATCCG ATATCATTTA AATCTAGGGA
    4601  TAACAGGGTA ATACTAGTGT CGACCAACCA GATAAGTGAA ATCTAGTTCC
    4651  AAACTATTTT GTCATTTTTA ATTTTCGTAT TAGCTTACGA CGCTACACCC
    4701  AGTTCCCATC TATTTTGTCA CTCTTCCCTA AATAATCCTT AAAAACTCCA
    4751  TTTCCACCCC TCCCAGTTCC CAACTATTTT GTCCGCCCAC ACCGTAAGAT
    4801  GCTTTTCTGT GACT


==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}


==Images {{HelpIcon|Help:Plasmid}}==
{{PlasmidImage}}


==References==
==References==
{{RefHelp}}
 
 
<references/>
<references/>


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[[Category:Plasmids]]
[[Category:Plasmids]][[Category:NEB Plasmids]]
[[Category:Kanamycin Marker Plasmids]][[Category:Tetracycline Marker Plasmids]]

Latest revision as of 18:37, 17 June 2010

At a Glance

pGPS1.1, Image provided by NEB
Type

Transposon Donor

Origin of Replication

R6K-γ[1]

Copy #

High

Markers

Tetracycline
Kanamycin

Link to Sequence

NEB

Features

Transprimer-1
Tn7R
Tn7L

Accession Number


edit table



Notes

pGPS1.1 is used as a trasposon donor in the GPS-1 Genome Priming System offered by NEB. TnsABC transposase removes the Tn7-based Transprimer element from pGPS1.1 and inserts it randomly into a trarget DNA molecule in vitro. pGPS1.1 is isolated from E. coli BW 23474, a strain that expresses the pir-116 replication factor (4).

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

New England Biolabs

Sequence

      1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 
     51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 
    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 
    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 
    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 
    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 
    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 
    351  GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT 
    401  AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA 
    451  CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC 
    501  ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA 
    551  GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG 
    601  TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA 
    651  CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA 
    701  AAGAGGTAAT GAAATGGCAG ATCCCTGGCT TGTTGTCCAC AACCGTTAAA 
    751  CCTTAAAAGC TTTAAAAGCC TTATATATTC TTTTTTTTCT TATAAAACTT 
    801  AAAACCTTAG AGGCTATTTA AGTTGCTGAT TTATATTAAT TTTATTGTTC 
    851  AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTTAGCCAT 
    901  GAGAGCTTAG TACGTTAGCC ATGAGGGTTT AGTTCGTTAA ACATGAGAGC 
    951  TTAGTACGTT AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA 
   1001  CGTACTATCA ACAGGTTGAA CTGCTGATCT TCGGATCTAT GTCGGGTGCG 
   1051  GAGAAAGAGG TAATGAAATG GCATCCGGAT CTGCATCGCA GGATGCTGCT 
   1101  GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCA TTATTGAAGC 
   1151  ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 
   1201  GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
   1251  CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG 
   1301  CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTCTCATGTT TGACAGCTTA 
   1351  TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC 
   1401  AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG 
   1451  CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC 
   1501  CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT 
   1551  GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT 
   1601  TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT 
   1651  CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC 
   1701  CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC 
   1751  AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC 
   1801  GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG 
   1851  GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC 
   1901  ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT 
   1951  TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA 
   2001  GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 
   2051  CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC 
   2101  CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG 
   2151  ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC 
   2201  TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG 
   2251  CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG 
   2301  TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC 
   2351  CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG 
   2401  ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC 
   2451  CTAACTTCGA TCATTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC 
   2501  GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC 
   2551  TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG 
   2601  ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA 
   2651  ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC 
   2701  TTGGCAGAAC ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC 
   2751  GCATCTCGGG CAGCGTTGGG TCCTGGGCTG GCATTGACCC TGAGTGATTT 
   2801  TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT 
   2851  TCCAGTAACC GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT 
   2901  CTCTCGTTTC ATCGGTATCA TTACCCCCAT GAACAGAAAT CCCCCTTACA 
   2951  CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA CATGGCCCGC 
   3001  TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA 
   3051  CGCGGATGAA CAGGCAGAGC TCTTACTGTC ATGCCATCCG TATGTGGGCG 
   3101  GACAATAAAG TCTTAAACTG AACAAAATAG ATCTAAACTA TGACAATAAA 
   3151  GTCTTAAACT AGACAGAATA GTTGTAAACT GAAATCAGTC CAGTTATGCT 
   3201  GTGAAAAAGC ATACTGGACT TTTGTTATGG CTAAAGCAAA CTCTTCATTT 
   3251  TCTGAAGTGC AAATTGCCCG TCGTATTAAA GAGGGGCGTG GGGTCGACGC 
   3301  GGCCGCTAAC TATAACGGTC CTAAGGTAGC GAGTTTAAAC GATATCGGAT 
   3351  CCGGCCGCCG CTGAGGTCTG CCTCGTGAAG AAGGTGTTGC TGACTCATAC 
   3401  CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT 
   3451  GATGAGAGCT TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT 
   3501  TTGCCACGGA ACGGTCTGCG TTGTCGGGAA GATGCGTGAT CTGATCCTTC 
   3551  AACTCAGCAA GAGTTCGATT TATTCAACAA AGCCGCCGTC CCGTCAAGTC 
   3601  AGCGTAATGC TCTGCCAGTG TTACAACCAA TTAACCAATT CTGATTAGAA 
   3651  AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT 
   3701  CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA 
   3751  CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC 
   3801  GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA 
   3851  GGTTATCAAG TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT 
   3901  GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT 
   3951  ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG 
   4001  ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA 
   4051  CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC 
   4101  AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT 
   4151  TCCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA 
   4201  AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT 
   4251  GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA 
   4301  GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA GATTGTCGCA 
   4351  CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC 
   4401  ATCCATGTTG GAATTTAATC GCGGCCTCGA GCAAGACGTT TCCCGTTGAA 
   4451  TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT 
   4501  ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT 
   4551  TTGAGACACA ACGTGGCTTA CTAGGATCCG ATATCATTTA AATCTAGGGA 
   4601  TAACAGGGTA ATACTAGTGT CGACCAACCA GATAAGTGAA ATCTAGTTCC 
   4651  AAACTATTTT GTCATTTTTA ATTTTCGTAT TAGCTTACGA CGCTACACCC 
   4701  AGTTCCCATC TATTTTGTCA CTCTTCCCTA AATAATCCTT AAAAACTCCA 
   4751  TTTCCACCCC TCCCAGTTCC CAACTATTTT GTCCGCCCAC ACCGTAAGAT 
   4801  GCTTTTCTGT GACT


References

  1. ↑ Stalker, DM et al. (1979) Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K. Proc. Natl. Acad. Sci. U.S.A. 76 1150-4 PubMed
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