PMID:6297458: Difference between revisions
Fill PMID: Page! |
imported>Wikientrybot change PMID template to TableEdit version |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{RightTOC}} | {{RightTOC}} | ||
< | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.180705.Q5223961a7ed04--> | ||
< | <!-- | ||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** --> | |||
{| id="Q5223961a7ed04" class=" tableEdit PMID_info_table" | |||
|- | |- | ||
| | !align=left |Citation | ||
|| | |||
'''Cammack, R, Jackson, RH, Cornish-Bowden, A and Cole, JA''' (1982) Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12. ''Biochem. J.'' '''207''':333-9 | |||
|- | |- | ||
| | !align=left |Abstract | ||
|| | |||
The NADH-dependent nitrite reductase of Escherichia coli, which contains sirohaem, flavin, non-haem iron and labile sulphide, was examined by low-temperature e.s.r. spectroscopy. The enzyme, stored in the presence of nitrite and ascorbate, gave the spectrum of a nitrosyl derivative, with hyperfine splitting due to the nitrosyl nitrogen. On removal of these reagents, a series of signals centred around g = 6 was observed, typical of high-spin ferric haem. Cyanide converted this into a low-spin form. On reduction of the enzyme with NADH, an axial spectrum at g = 1.92, 2.01 was observed. The temperature-dependence of this signal is indicative of a [2Fe-2S] iron-sulphur cluster. The midpoint potential of this cluster was estimated to be -230 +/- 15 mV by two independent methods. Reduction of the enzyme with dithionite yielded further signals, which are at present unidentified, at g = 2.1-2.28. No signals were observed that could be assigned to a [4Fe-4S] cluster, such as is found in other sulphite reductases and nitrite reductases that contain sirohaem. | |||
|- | |||
!align=left |Links | |||
|| | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6297458 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153865 PMC1153865] | |||
|- | |||
!align=left |Keywords | |||
|| | |||
Electron Spin Resonance Spectroscopy; Escherichia coli/enzymology; Heme/analogs & derivatives; Heme/metabolism; Iron/metabolism; NADH, NADPH Oxidoreductases/metabolism; Nitrite Reductase (NAD(P)H); Nitrite Reductases/metabolism; Oxidation-Reduction; Potassium Cyanide/pharmacology; Sulfur/metabolism; Thiosulfates/pharmacology | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180705.Q5223961a7ed04&page=180705&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> || | |||
|} | |} | ||
< | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.180705.Q5223961a7ed04--> | ||
==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| Line 19: | Line 38: | ||
==Annotations {{HelpIcon|Help:Literature}}== | ==Annotations {{HelpIcon|Help:Literature}}== | ||
<protect>< | <protect><annotationlinks/></protect> | ||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:49, 15 September 2013
| Citation |
Cammack, R, Jackson, RH, Cornish-Bowden, A and Cole, JA (1982) Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12. Biochem. J. 207:333-9 |
|---|---|
| Abstract |
The NADH-dependent nitrite reductase of Escherichia coli, which contains sirohaem, flavin, non-haem iron and labile sulphide, was examined by low-temperature e.s.r. spectroscopy. The enzyme, stored in the presence of nitrite and ascorbate, gave the spectrum of a nitrosyl derivative, with hyperfine splitting due to the nitrosyl nitrogen. On removal of these reagents, a series of signals centred around g = 6 was observed, typical of high-spin ferric haem. Cyanide converted this into a low-spin form. On reduction of the enzyme with NADH, an axial spectrum at g = 1.92, 2.01 was observed. The temperature-dependence of this signal is indicative of a [2Fe-2S] iron-sulphur cluster. The midpoint potential of this cluster was estimated to be -230 +/- 15 mV by two independent methods. Reduction of the enzyme with dithionite yielded further signals, which are at present unidentified, at g = 2.1-2.28. No signals were observed that could be assigned to a [4Fe-4S] cluster, such as is found in other sulphite reductases and nitrite reductases that contain sirohaem. |
| Links | |
| Keywords |
Electron Spin Resonance Spectroscopy; Escherichia coli/enzymology; Heme/analogs & derivatives; Heme/metabolism; Iron/metabolism; NADH, NADPH Oxidoreductases/metabolism; Nitrite Reductase (NAD(P)H); Nitrite Reductases/metabolism; Oxidation-Reduction; Potassium Cyanide/pharmacology; Sulfur/metabolism; Thiosulfates/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.