PMID:6297458
| Citation |
Cammack, R, Jackson, RH, Cornish-Bowden, A and Cole, JA (1982) Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12. Biochem. J. 207:333-9 |
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| Abstract |
The NADH-dependent nitrite reductase of Escherichia coli, which contains sirohaem, flavin, non-haem iron and labile sulphide, was examined by low-temperature e.s.r. spectroscopy. The enzyme, stored in the presence of nitrite and ascorbate, gave the spectrum of a nitrosyl derivative, with hyperfine splitting due to the nitrosyl nitrogen. On removal of these reagents, a series of signals centred around g = 6 was observed, typical of high-spin ferric haem. Cyanide converted this into a low-spin form. On reduction of the enzyme with NADH, an axial spectrum at g = 1.92, 2.01 was observed. The temperature-dependence of this signal is indicative of a [2Fe-2S] iron-sulphur cluster. The midpoint potential of this cluster was estimated to be -230 +/- 15 mV by two independent methods. Reduction of the enzyme with dithionite yielded further signals, which are at present unidentified, at g = 2.1-2.28. No signals were observed that could be assigned to a [4Fe-4S] cluster, such as is found in other sulphite reductases and nitrite reductases that contain sirohaem. |
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| Keywords |
Electron Spin Resonance Spectroscopy; Escherichia coli/enzymology; Heme/analogs & derivatives; Heme/metabolism; Iron/metabolism; NADH, NADPH Oxidoreductases/metabolism; Nitrite Reductase (NAD(P)H); Nitrite Reductases/metabolism; Oxidation-Reduction; Potassium Cyanide/pharmacology; Sulfur/metabolism; Thiosulfates/pharmacology |
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