PMID:7028742: Difference between revisions

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<span style = 'font-size: large'> '''Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR'''  (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli.''J. Biol. Chem.'' '''256''':11804-8</span>
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|{{TableHeadingColor}} |'''Abstract''' || <protect>The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow.</protect>
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'''Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR''' (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. ''J. Biol. Chem.'' '''256''':11804-8
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!align=left  |Abstract
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7028742 PubMed]
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The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7028742 PubMed]


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Amino Acids/analysis; Cell Membrane/enzymology; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics; Membrane Potentials; Membrane Transport Proteins/isolation & purification; Membrane Transport Proteins/metabolism; Molecular Weight; Monosaccharide Transport Proteins; Symporters
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==Annotations {{HelpIcon|Help:Literature}}==
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
 
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Latest revision as of 04:49, 15 September 2013

Citation

Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. J. Biol. Chem. 256:11804-8

Abstract

The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow.

Links

PubMed

Keywords

Amino Acids/analysis; Cell Membrane/enzymology; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics; Membrane Potentials; Membrane Transport Proteins/isolation & purification; Membrane Transport Proteins/metabolism; Molecular Weight; Monosaccharide Transport Proteins; Symporters

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