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{{See|Help:Plasmid|plasmids}}
==At a Glance==
==At a Glance==
[[File:PBAD33.jpg|thumb|450px|pBAD33 Image made with [[PlasMapper]]]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.173069.K4c3f76863c603-->
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.173069.K4c3f76863c603-->
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!align=left {{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
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pACYC184
pACYC184/p15A
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|-
!align=left {{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
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!align=left {{table heading style}} |Markers
!align=left {{table heading style}} |Markers
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CamR
|-
|-
!align=left {{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
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[https://www.lablife.org/g?a=seqa&id=vdb%5fg2%2ejPxWBK9aksPdtGbxXRrF06TxA%2e4%2d%5fsequence%5f549a5591ef126b8ee61fb291e3498cd6c3147325%5f10 LabLife]
|-
|-
!align=left {{table heading style}} |Features
!align=left {{table heading style}} |Features
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''araC''
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!align=left {{table heading style}} |Accession Number
!align=left {{table heading style}} |Accession Number
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==Sources==
==Sources==
[http://www.atcc.org/ ATCC]
{{PlasmidSources}}
{{PlasmidSources}}


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{{PlasmidSeq}}
{{PlasmidSeq}}


<pre>
    1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
  61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
  121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
  181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
  241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
  301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
  361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
  421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
  481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
  541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
  601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
  661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
  721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
  781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
  841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
  901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
  961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1981 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa
2041 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
2101 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
2161 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
2221 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
2281 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc
2341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
2401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
2461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
2521 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
2581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
2641 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
2701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
2761 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata
2821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
3121 tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
3181 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
3241 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
3301 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
3361 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
3421 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
3481 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
3541 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
3601 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
3661 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
3721 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
3781 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
3841 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
3901 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
3961 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
4021 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
4081 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
4141 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
4201 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
4261 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
4321 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
4381 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
4441 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
4501 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
4561 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
4621 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
4681 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
4741 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
4801 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
4861 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
4921 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
4981 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
5041 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
5101 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
5161 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttg ctcatgagcc
5221 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
5281 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt
5341 tgacagctta tc
</pre>


[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Chloramphenicol Marker Plasmids]][[Category:araBAD Promoter]][[Category:araC]]
[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Chloramphenicol Marker Plasmids]][[Category:araBAD Promoter]][[Category:araC]]
__NOTOC__
__NOTOC__

Latest revision as of 20:13, 13 September 2011

At a Glance

pBAD33 Image made with PlasMapper
Type

Cloning Expression Vector

Origin of Replication

pACYC184/p15A

Copy #
Markers

CamR

Link to Sequence

LabLife

Features

araC

Accession Number


edit table


Notes

History

Construction described in Guzman et al. (1995)[1].

Sources

ATCC

You can help EcoliWiki by writing about the available sources of this plasmid.

References

See Help:References for how to manage references in EcoliWiki.

  1. ↑ Guzman, LM et al. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177 4121-30 PubMed

Sequence

You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid. 

    1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 
   61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 
  121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 
  181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 
  241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 
  301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 
  361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 
  421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 
  481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 
  541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 
  601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 
  661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 
  721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 
  781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 
  841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 
  901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 
  961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 
 1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 
 1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 
 1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 
 1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 
 1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 
 1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 
 1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 
 1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 
 1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 
 1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 
 1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 
 1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 
 1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 
 1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
 1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
 1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
 1981 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa 
 2041 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 
 2101 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 
 2161 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 
 2221 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 
 2281 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc 
 2341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
 2401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
 2461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 
 2521 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc 
 2581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
 2641 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat 
 2701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
 2761 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata 
 2821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
 2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
 2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
 3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
 3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
 3121 tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa 
 3181 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac 
 3241 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg 
 3301 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc 
 3361 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga 
 3421 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 
 3481 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 
 3541 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 
 3601 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 
 3661 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 
 3721 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 
 3781 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 
 3841 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 
 3901 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 
 3961 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 
 4021 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 
 4081 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct 
 4141 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag 
 4201 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga 
 4261 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct 
 4321 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta 
 4381 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag 
 4441 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc 
 4501 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg 
 4561 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg 
 4621 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca 
 4681 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc 
 4741 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg 
 4801 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct 
 4861 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg 
 4921 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg 
 4981 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt 
 5041 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa 
 5101 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca 
 5161 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttg ctcatgagcc 
 5221 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg 
 5281 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt 
 5341 tgacagctta tc
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