MG1655 seqA-eYFP: Difference between revisions

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| Sources
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| [http://bccm.belspo.be/catalogues/files/genecorner-hosts/MG1655%20seqA-eYFP.pdf LMBP 9776]  
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/660 LMBP 9776]  
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| References
| References

Latest revision as of 11:30, 28 October 2024

Name MG1655 seqA-eYFP
Synonyms
Genotype F– λ– ilvG– rfb-50 rph-1 seqA-eYFP:kmR
Phenotype Kanamycin resistant
Sources LMBP 9776
References PMID:26449690

Notes

The native seqA gene was fused at its 3' end to the fluorescent protein gene eYFP, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.