Category:Strain:S26 R1e: Difference between revisions
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!align=left {{table_heading_style}} |Name | !align=left {{table_heading_style}} |Name | ||
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S26 R1e | |||
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!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
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K37 | |||
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!align=left {{table_heading_style}} |Genotype | !align=left {{table_heading_style}} |Genotype | ||
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Hfr (PO2A) ''garB10 fhuA22 phoA4(Am) ompF627(T2R) serU132(AS) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510'' | |||
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!align=left {{table_heading_style}} |Phenotype | !align=left {{table_heading_style}} |Phenotype | ||
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phosphatase positive | |||
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!align=left {{table_heading_style}} |Plasmids | !align=left {{table_heading_style}} |Plasmids | ||
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Hfr | |||
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!align=left {{table_heading_style}} |Phage | !align=left {{table_heading_style}} |Phage | ||
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λ<sup>+</sup> | |||
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!align=left {{table_heading_style}} |Sources | !align=left {{table_heading_style}} |Sources | ||
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[http://cgsc.biology.yale.edu/Strain.php?ID=11298 CGSC:2598]<br /> | |||
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/343 LMBP 4978] | |||
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!align=left {{table_heading_style}} |Construction | !align=left {{table_heading_style}} |Construction | ||
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Strain S26 R1e is a spontaneous isolate of strain S26 | |||
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!align=left {{table_heading_style}} |References | !align=left {{table_heading_style}} |References | ||
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PMID:5327650 PMID:4564480 PMID:14337506 | |||
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== Notes == | == Notes == | ||
This strain carries the S26 amber mutation (TAG) in the phosphatase gene. <br /> | |||
<br /> | |||
This strain carries the supD suppressor (inserts serine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon. | |||
==References== | ==References== | ||
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[[Category:Strains]] | [[Category:Strains]][[Category:BCCM/GeneCorner]] | ||
Latest revision as of 11:10, 28 October 2024
See Help:Strains for more information about strains in EcoliWiki.
| Name |
S26 R1e |
|---|---|
| Synonyms |
K37 |
| Genotype |
Hfr (PO2A) garB10 fhuA22 phoA4(Am) ompF627(T2R) serU132(AS) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510 |
| Phenotype |
phosphatase positive |
| Plasmids |
Hfr |
| Phage |
λ+ |
| Sources | |
| Construction |
Strain S26 R1e is a spontaneous isolate of strain S26 |
| References | |
Notes
This strain carries the S26 amber mutation (TAG) in the phosphatase gene.
This strain carries the supD suppressor (inserts serine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Garen, A et al. (1965) Suppressor genes for nonsense mutations. I. The Su-1, Su-2 and Su-3 genes of Escherichia coli. J. Mol. Biol. 14 167-78 PubMed
- ↑ Remaut, E & Fiers, W (1972) Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron. J. Mol. Biol. 71 243-61 PubMed
- ↑ WEIGERT, MG & GAREN, A (1965) AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J. Mol. Biol. 12 448-55 PubMed
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