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[[Category:Databases]]
[[File:Addgene New Logo.jpg|center]]
[[File:Addgene New Logo.jpg|center]]
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[http://www.addgene.org/vector-database/ www.Addgene.org]
[http://www.addgene.org/vector-database/ www.Addgene.org]
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!align=left {{table heading style}} |What:
!align=left {{table heading style}} |What:
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Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
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!align=left {{table heading style}} |Who:
!align=left {{table heading style}} |Who:
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Addgene is a non-profit global plasmid repository dedicated to helping scientists share their plasmids.  
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.  
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!align=left {{table heading style}} |Updates:
!align=left {{table heading style}} |Updates:
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Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
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!align=left {{table heading style}} |Upcoming events:
!align=left {{table heading style}} |Upcoming events:
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Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
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!align=left {{table heading style}} |Web Services:
!align=left {{table heading style}} |Web Services:
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[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.
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{{Database_table_help}}
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a non-profit organization dedicated to making it easier for scientists to share plasmids.
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.


Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.
Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.


==Content {{HelpIcon|Help:Databases}}==
==Content{{HelpIcon|Help:Databases}}==
Addgene is a not-for-profit plasmid repository with an online database of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by [http://www.addgene.org/ Addgene]. Only the plasmids deposited by scientists at [http://www.addgene.org/ Addgene] are available for purchase through this website.
'''Plasmid Repository'''
 
As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.
 
The plasmid collection includes:
 
* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression
 
==Tools and guides==
 
Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.
 
'''Molecular Biology Tools'''
 
[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.
 
[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool  developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.
 
[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.
 
'''Plasmid Cloning Guides'''
 
[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.
 
[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.


==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).
===Browsing===
===Browsing===
[http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.
 
Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. Browse Plasmid Repository by plasmid function or type [http://www.addgene.org/vector-database/ Browse Plasmid Repository].
 


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[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]  
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]  
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[http://www.addgene.org/search/advanced/?q=&depositor=&article=&gene=&vector=&tags=&advanced_query=&results_per_page=20&page=1&selected_facets=plasmid_type_exact%3A7&sort_type=relevance Bacterial Strain]
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===Searching===
===Searching===
Search Addgene's collection by plasmid source, slectable marker, or bacterial resistance.
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===Usage examples===
''Add links to additional pages describing success stories here.''
==Other sites with related content {{HelpIcon|Help:Databases}}==
==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''
In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs.<ref>{{cite web|last=Baker|first=Monya|title=Repositories share key research tools|url=http://www.nature.com/news/repositories-share-key-research-tools-1.14511|publisher=Nature.com|accessdate=8 May 2014}}</ref>
Addgene’s plasmid collection contains plasmids used for a variety of functions such as  [[genome engineering]] (including CRISPRS), [[gene expression]], [[shRNA]] knockdown, viral-mediated gene delivery, detection of [[miRNA]] and promoter activity.
The plasmid collection includes:
* Genome engineering
** [[CRISPR]]s
** [[Transcription Activator-Like Effector Nuclease]] (TALEN) kits
** [[Zinc finger nuclease]] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [[Fluorescent marker]]
* Viral vectors
** Retroviral/Lentiviral
** [[Adenoviral]]
** [[Adeno-associated virus|AAV]]
* [[cDNA]] expression
* shRNA expression
==Tools and guides==
'''Molecular Biology Tools'''
[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.
[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool  developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.
[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.
'''Plasmid Cloning Guides'''
[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.
[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.
==Discussion {{HelpIcon|Help:Databases}}==
==References {{HelpIcon|Help:Databases}}==
<references />
[http://www.addgene.org/ www.addgene.org]
[http://www.addgene.org/vector-database/ Addgene's Vector Database]
==External Links==
[{{{1|link}}} {{PAGENAME}}] URL:{{{1|link}}}
===Discussion of {{PAGENAME}} on other websites===

Latest revision as of 14:07, 22 July 2014

You can help EcoliWiki by editing the content of this page. For information about becoming a registered user and obtaining editing privileges, see Help:Accounts.

Link/URL:

www.Addgene.org

What:

Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) Click to search vector database now.

Who:

Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.

Updates:

Do you use plasmids?

Join the non-profit plasmid sharing community. Visit www.addgene.org to learn more, find plasmids, and start depositing!

Upcoming events:

Visit Addgene's News and Announcements page to see current news and announcements.

Web Services:

Addgene is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

edit table

See Help:Database_table for how to add or edit information in this section of EcoliWiki.

About Addgene

Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

Content

Plasmid Repository

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

Tools and guides

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

Molecular Biology Tools

Vector Database- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

Sequence Analyzer- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

Molecular Biology Reference- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

Plasmid Cloning Guides

Molecular Cloning Guides- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

Molecular Cloning Protocols- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

Using Addgene

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

Browsing

Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. Browse Plasmid Repository by plasmid function or type Browse Plasmid Repository.


Browse By Plasmid Type:

Mammalian Expression

Bacterial Expression

Luciferase

Mouse Targeting

Retroviral

Lentiviral

Worm Expression

Insect Expression

Yeast Expression, E.Coli or yeast

RNAi

Bacterial Strain


edit table


Searching

Search Addgene's collection by plasmid source, slectable marker, or bacterial resistance.

Additional Searches By:

Bacterial Resistance

Ampicillin; Kanamycin; Tetracycline; Chloramphenicol; Hygromycin; Spectinomycin; Gentamycin

Selectable Marker

Neomycin; Puromycin; Hygromycin; Zeocin; URA3; LEU2; TRP1;HIS3

Source

Addgene; ATCC; BD Biosciences; Invitrogen; Novagen; Sigma; Stratagene

edit table
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