PMID:7028742: Difference between revisions
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!align=left |Citation | |||
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'''Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR''' (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. ''J. Biol. Chem.'' '''256''':11804-8 | |||
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!align=left |Abstract | |||
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The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow. | |||
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!align=left |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7028742 PubMed] | |||
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!align=left |Keywords | |||
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Amino Acids/analysis; Cell Membrane/enzymology; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics; Membrane Potentials; Membrane Transport Proteins/isolation & purification; Membrane Transport Proteins/metabolism; Molecular Weight; Monosaccharide Transport Proteins; Symporters | |||
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==Significance {{HelpIcon|Help:Literature}} == | |||
{{LitSignificance}} | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | |||
{{LitMaterials}} | |||
==Annotations {{HelpIcon|Help:Literature}}== | |||
<protect><annotationlinks/></protect> | |||
==EcoliWiki Links {{HelpIcon|Help:Literature}} == | |||
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==See also== | |||
==References== | |||
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Latest revision as of 04:49, 15 September 2013
| Citation |
Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. J. Biol. Chem. 256:11804-8 |
|---|---|
| Abstract |
The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow. |
| Links | |
| Keywords |
Amino Acids/analysis; Cell Membrane/enzymology; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics; Membrane Potentials; Membrane Transport Proteins/isolation & purification; Membrane Transport Proteins/metabolism; Molecular Weight; Monosaccharide Transport Proteins; Symporters |
| edit table |
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