PMID:4598003: Difference between revisions
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'''Beyersmann, D, Messer, W and Schlicht, M''' (1974) Mutants of Escherichia coli B-r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication. ''J. Bacteriol.'' '''118''':783-9 | |||
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!align=left |Abstract | |||
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Mutagenized E. coli B/r cells were subjected to a procedure designed to select mutants temperature-sensitive for initiation of deoxyribonucleic acid (DNA) replication. Seventeen mutants exhibiting limited residual DNA synthesis at 42 C were obtained and the dna(-) sites were mapped genetically. Sixteen of the sites map near dnaA, dnaB, and dnaC. One mutant (dna-208) maps in a new location between the trp and his genes. We propose to call this mutant dnaI208. In complementation experiments dnaC(+) and dnaI(+) were dominant to dnaC(-) and dnaI(-) alleles, respectively. However, dnaA(-) was dominant to the wild-type allele dnaA(+). All dnaA mutants and four out of six dnaC mutants could be suppressed by F factor integration. The pattern of suppression was specific for each mutant. | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4598003 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC246822 PMC246822] | |||
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| | !align=left |Keywords | ||
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|{{ | Bacterial Proteins/biosynthesis; Carbon Radioisotopes; Cell Count; Chromosome Mapping; Conjugation, Genetic; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Genetic Complementation Test; Leucine/metabolism; Mutagens; Mutation; Nitrosoguanidines; Suppression, Genetic; Temperature; Thymine/metabolism; Transduction, Genetic; Tritium | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
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==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ==EcoliWiki Links {{HelpIcon|Help:Literature}} == | ||
Latest revision as of 04:47, 15 September 2013
| Citation |
Beyersmann, D, Messer, W and Schlicht, M (1974) Mutants of Escherichia coli B-r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication. J. Bacteriol. 118:783-9 |
|---|---|
| Abstract |
Mutagenized E. coli B/r cells were subjected to a procedure designed to select mutants temperature-sensitive for initiation of deoxyribonucleic acid (DNA) replication. Seventeen mutants exhibiting limited residual DNA synthesis at 42 C were obtained and the dna(-) sites were mapped genetically. Sixteen of the sites map near dnaA, dnaB, and dnaC. One mutant (dna-208) maps in a new location between the trp and his genes. We propose to call this mutant dnaI208. In complementation experiments dnaC(+) and dnaI(+) were dominant to dnaC(-) and dnaI(-) alleles, respectively. However, dnaA(-) was dominant to the wild-type allele dnaA(+). All dnaA mutants and four out of six dnaC mutants could be suppressed by F factor integration. The pattern of suppression was specific for each mutant. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Carbon Radioisotopes; Cell Count; Chromosome Mapping; Conjugation, Genetic; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Genetic Complementation Test; Leucine/metabolism; Mutagens; Mutation; Nitrosoguanidines; Suppression, Genetic; Temperature; Thymine/metabolism; Transduction, Genetic; Tritium |
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