PMID:4598003
| Citation |
Beyersmann, D, Messer, W and Schlicht, M (1974) Mutants of Escherichia coli B-r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication. J. Bacteriol. 118:783-9 |
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| Abstract |
Mutagenized E. coli B/r cells were subjected to a procedure designed to select mutants temperature-sensitive for initiation of deoxyribonucleic acid (DNA) replication. Seventeen mutants exhibiting limited residual DNA synthesis at 42 C were obtained and the dna(-) sites were mapped genetically. Sixteen of the sites map near dnaA, dnaB, and dnaC. One mutant (dna-208) maps in a new location between the trp and his genes. We propose to call this mutant dnaI208. In complementation experiments dnaC(+) and dnaI(+) were dominant to dnaC(-) and dnaI(-) alleles, respectively. However, dnaA(-) was dominant to the wild-type allele dnaA(+). All dnaA mutants and four out of six dnaC mutants could be suppressed by F factor integration. The pattern of suppression was specific for each mutant. |
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| Keywords |
Bacterial Proteins/biosynthesis; Carbon Radioisotopes; Cell Count; Chromosome Mapping; Conjugation, Genetic; DNA Replication; DNA, Bacterial/biosynthesis; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Genetic Complementation Test; Leucine/metabolism; Mutagens; Mutation; Nitrosoguanidines; Suppression, Genetic; Temperature; Thymine/metabolism; Transduction, Genetic; Tritium |
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