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{{Tab On|Quickview|glnK}}{{Tab|Gene|glnK}}{{Tab|Gene Product(s)|glnK}}{{Tab|Expression|glnK}}{{Tab|Evolution|glnK}}{{Tab|On One Page|glnK}}{{TabsBottom}}
{{Tab On|Quickview|glnK}}{{Tab|Gene|glnK}}{{Tab|Gene Product(s)|glnK}}{{Tab|Expression|glnK}}{{Tab|Evolution|glnK}}{{Tab|On One Page|glnK}}{{TabsBottom}}
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!{{table_heading_style}} |Gene Name
!align=left {{table_heading_style}} |Standard Name
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[[glnK:Gene|''glnK]]'' (synonyms: ''ECK0444, b0450, JW0440, ybaI'')<ref name='LIB:Riley_2006'/>
''glnK''
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!{{table_heading_style}} |Product(s)  
!align=left {{table_heading_style}} |Gene Synonym(s)
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[[glnK:Gene_Product(s)|GlnK product, GlnK product]]<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>: B0450 product, YbaI product, unmodified protein PII 2 product, [protein PII2] product, PII2 product
''ECK0444, b0450, JW0440, ybaI''<ref name='LIB:Riley_2006'/>, ybaI''
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!{{table_heading_style}} |Description
!align=left {{table_heading_style}} |Product Desc.
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[GlnK]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>  
[GlnK]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> , uridylyl-[GlnK]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


Component of [[:Category:Complex:nitrogen regulatory protein GlnK|nitrogen regulatory protein GlnK]]<ref name='LIB:EcoCyc10.6'/>
Component of [[:Category:Complex:nitrogen regulatory protein GlnK|nitrogen regulatory protein GlnK]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


Potent activator of NRII(GlnL/NtrB) phosphatase; trimeric; regulated through NRI/NRII two-component regulatory system; binds to membrane when inhibiting AmtB<ref name='LIB:EcoGene'/>
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!align=left {{table_heading_style}} |Product Synonyms(s)
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''nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB<ref name='LIB:Riley_2006'/>, B0450<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YbaI<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>,unmodified protein PII 2<ref name='LIB:EcoCyc10.6'/>, modified protein PII 2<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>,[protein PII2]<ref name='LIB:EcoCyc10.6'/>, uridylyl-[protein PII2]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>,PII2<ref name='LIB:EcoCyc10.6'/>, uridylyl-[PII2]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, , ECK0444, JW0440, ybaI, b0450''
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!align=left {{table_heading_style}} |Function from GO
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<GO_nr />
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!{{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
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!{{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
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transcription unit(s): [[:Category:Txn_unit:glnK-amtB|glnK-amtB]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:glnK-amtB|glnK-amtB]]<ref name='LIB:EcoCyc10.6'/>  
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!{{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
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!align=left {{table_heading_style}} |Quick Links
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[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=glnK&s=search]] [[File:EcoCyc.gif|link=http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG12191]] [[File:regulondb.jpg|link=http://regulondb.ccg.unam.mx/gene?term=ECK120002080&organism=ECK12&format=jsp&type=gene ]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=glnK&loc=MG1655%3A471822..472160,W3110%3A471822..472160,DH10B%3A411153..411491,REL606%3A444665..445003,BW2952%3A374581..374919]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=374581&end=374919&gene=glnK&aa=mklvtviikpfkledvrealssigiqgltvtevkgfgrqkghaelyrgaeysvnflpkvkidvaiaddqldevidivskaaytgkigdgkifvaelqrvirirtgeadeaal]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=glnK+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=glnK]]&nbsp;&nbsp;
 
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==Notes==
===Notes===
{{BacktoTop}}
 
glnK is a glnB paralog. Like GlnB, the GlnK protein is a member of the trimeric PII signal transduction protein family. In nitrogen limitation it is subject to post-translational modification by uridylylation (mediated by GlnD) of Tyr51 in the T-loop of each subunit. This modification is reversed in N-sufficient conditions.  In ammonium sufficient conditions non-uridylylated GlnK forms a 1:1 complex with the ammonia channel protein AmtB and prevents ammonium uptake through AmtB.  Complex formation is reversed by ammonium starvation. [Comment provided by EcoTeam member Mike Merrick, 11.16.06]<ref name='LIB:EcoGene'/>
 




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==References==
==References==
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Latest revision as of 21:50, 27 May 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

glnK

Gene Synonym(s)

ECK0444, b0450, JW0440, ybaI[1], ybaI

Product Desc.

[GlnK][2][3] , uridylyl-[GlnK][2][3]

Component of nitrogen regulatory protein GlnK[2][3]

Potent activator of NRII(GlnL/NtrB) phosphatase; trimeric; regulated through NRI/NRII two-component regulatory system; binds to membrane when inhibiting AmtB[4]

Product Synonyms(s)

nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB[1], B0450[2][1], YbaI[2][1],unmodified protein PII 2[2], modified protein PII 2[2][1],[protein PII2][2], uridylyl-[protein PII2][2][1],PII2[2], uridylyl-[PII2][2][1], , ECK0444, JW0440, ybaI, b0450

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): glnK-amtB[2]

Regulation/Activity
Quick Links



        

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Notes

[back to top]


glnK is a glnB paralog. Like GlnB, the GlnK protein is a member of the trimeric PII signal transduction protein family. In nitrogen limitation it is subject to post-translational modification by uridylylation (mediated by GlnD) of Tyr51 in the T-loop of each subunit. This modification is reversed in N-sufficient conditions. In ammonium sufficient conditions non-uridylylated GlnK forms a 1:1 complex with the ammonia channel protein AmtB and prevents ammonium uptake through AmtB. Complex formation is reversed by ammonium starvation. [Comment provided by EcoTeam member Mike Merrick, 11.16.06][4]



References

[back to top]


  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
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