DH10B: Difference between revisions

Latest revision as of 03:22, 15 September 2012

See Help:Strains for more information about strains in EcoliWiki.

Alternate Names

Sources

Chromosomal Genotype

F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ^-
From J Jessee.^[1]

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Comments

useful for generating genomic libraries containing methylated cytosine or adenine residues; useful for plasmid rescue procedures (Copied from GIBCO)

The sequence of DH10B was published in 2008^[2] .

References

See Help:References for how to manage references in EcoliWiki.

↑ Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed
↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed

[PMID:2162051-1] Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed

[PMID:18245285-2] Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed

[1]

[2]

@@ Line 1: / Line 1: @@
-== Strain ==
+{{See|Help:Strains|strains}}
-DH10B
-== Genotype ==
-F<sup>-</sup> ''mcr''A Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup><br />
+== Alternate Names ==
+==Sources ==
+==Chromosomal Genotype ==
+F<sup>-</sup> [[mcrA]] Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup><br />
 From J Jessee.<ref name='PMID:2162051'/>
+==Plasmids ==
+not specified
+==Phage ==
+not specified
+==Phenotypes ==
+==Construction ==
+unknown parents.
 == Comments ==
+useful for generating genomic libraries containing methylated cytosine or adenine residues; useful for plasmid rescue procedures (Copied from GIBCO)
-host for pUC and other α-complementation vectors; pBR322<br />
+The sequence of DH10B was published in 2008<ref name='PMID:18245285'/> .
-useful for generating genomic libraries containing methylated cytosine or adenine residues<br />
-useful for plasmid rescue procedures
-Copyed from GIBCO
+== See Also ==
+* [[:Category:Gene_List:DH10B]]
 == References ==
+{{RefHelp}}<references/>
-. S G Grant, J Jessee, F R Bloom, and D Hanahan. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci. 1990. 87(12):4645–4649.
+__NOTOC__
-<references/>
+[[Category:Strains]]