pETBlue-2: Difference between revisions

Latest revision as of 20:43, 13 September 2011

At a Glance

Type	Expression
Origin of Replication	pUC, f1
Copy #	high
Markers	Ampicillin
Link to Sequence	Novagen
Features	lac operator T7 promoter multiple cloning site His•Tag HSV•Tag lacZ start codon E. coli promoter
Accession Number
edit table

Notes

pETBlue-2 provides a vector-encoded ATG start codon and a variety of downstream cloning sites.

History

The pETBlue™ System combines the visual identification of recombinants and high plasmid copy number, found in popular blue/white screening vectors, with the tightly controlled high yield protein expression obtained with pET vectors.

Sources

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

Sequence

       1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gacttacaat 
      61 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtacg ggcctcttcg 
     121 ctattacgcc agcttgcgaa cggtgggtgc gctgcaaggc gattaagttg ggtaacgcca 
     181 ggattctccc agtcacgacg ttgtaaaacg acggccagcg agagatcttg attggctagc 
     241 agaataattt tgtttaactt taagaaggag atataccatg gcgatatccc gggagctcgt 
     301 ggatccgaat tctgtacagg cgcgcctgca ggacgtcgac ggtaccatcg atacgcgttc 
     361 gaagcttgcg gccgcacagc tgtatacacg tgcaagccag ccagaactcg ctcctgaaga 
     421 cccagaggat ctcgagcacc accaccacca ccactaatgt taattaagtt gggcgttgta 
     481 atcatagtca taatcaatac tcctgactgc gttagcaatt taactgtgat aaactaccgc 
     541 attaaagcta ttcgatgata agctgtcaaa catgataatt cttgaagacg aaagggccta 
     601 ggctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc 
     661 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag 
     721 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 
     781 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat 
     841 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc 
     901 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact 
     961 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctgagcaata actagcataa 
    1021 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc 
    1081 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
    1141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
    1201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
    1261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
    1321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
    1381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
    1441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
    1501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca 
    1561 cgatggcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
    1621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
    1681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
    1741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
    1801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
    1861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
    1921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
    1981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
    2041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
    2101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
    2161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
    2221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
    2281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
    2341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
    2401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
    2461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
    2521 actcatactc ttcctttttc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac 
    2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
    2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
    2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
    2761 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
    2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
    2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
    2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
    3001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
    3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
    3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
    3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
    3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
    3301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 
    3361 agcgagtcag tgagcgagga agccggcgat aatggcctgc ttctcgccga aacgtttggt 
    3421 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga 
    3481 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc 
    3541 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgac 
    3601 cggtgaattg tgagcgctca caattctcgt gacatcataa cgtcccgcga aat

@@ Line 1: / Line 1: @@
-==At a Glance {{HelpIcon|Help:Plasmid}}==
+==At a Glance ==
+[[File:PETBlue-2.jpg|thumb|450px|pETBlue-2, Image made with [[PlasMapper]]]]
 <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb-->
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 ******************************************************************************************
 *
 *   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 *
 ****************************************************************************************** -->
-{|{{Prettytable}} width='30%' align=left
+{| {{Prettytable}} width='30%' align=left  id="M475ff9fa4e4eb"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
+||
+Expression
 |-
-!{{table heading style}} |Origin of Replication
+!align=left {{table heading style}} |Origin of Replication
+||
+pUC, f1
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Copy #
+||
+high
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Markers
+||
+Ampicillin
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Link to Sequence
+||
+[http://www.emdbiosciences.com/usa/life-science-research/vector-table-petblue-2-vector-sequence/c_276b.s1Onz4AAAEhHtsLdeSQ?WFSimpleSearch_NameOrID=pETBlue-2+sequence&BackButtonText=search+results Novagen]
 |-
-!{{table heading style}} |Features
+!align=left {{table heading style}} |Features
+||
+lac operator <br />
+T7 promoter<br />
+multiple cloning site <br />
+His•Tag<br />
+HSV•Tag <br />
+lacZ start codon <br />
+E. coli promoter<br />
 |-
-!{{table heading style}} |Accession Number
+!align=left {{table heading style}} |Accession Number
-|-
+||
-|colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb&page=0&pagename={{FULLPAGENAMEE}} edit table]
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb&page=114185&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb--></protect>
 <BR>
 <Br clear='all'>
-===Notes===
-==History {{HelpIcon|Help:Plasmid}}==
+==Notes==
-{{PlasmidHistory}}
+pETBlue-2 provides a vector-encoded ATG start codon and a variety of downstream cloning sites.
+==History ==
+The pETBlue™ System combines the visual identification of recombinants and high plasmid copy number, found in popular blue/white screening vectors, with the tightly controlled high yield protein expression obtained with pET vectors.
-==Sources {{HelpIcon|Help:Plasmid}}==
+==Sources ==
-{{PlasmidSources}}
+[http://www.merck-chemicals.com/life-science-research/petblue-2-perfectly-blunt-cloning-kit/EMD_BIO-70636/p_eHeb.s1OncgAAAEj7xt9.zLX?WFSimpleSearch_NameOrID=pETBlue-2&BackButtonText=search+results Novagen]
 ==References==
+{{BacktoTop}}
 {{RefHelp}}
-<references/>
+<references />
-==Sequence {{HelpIcon|Help:Plasmid}}==
-{{PlasmidSeq}}
+==Sequence ==
+taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gacttacaat
+ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtacg ggcctcttcg
+ctattacgcc agcttgcgaa cggtgggtgc gctgcaaggc gattaagttg ggtaacgcca
+ggattctccc agtcacgacg ttgtaaaacg acggccagcg agagatcttg attggctagc
+agaataattt tgtttaactt taagaaggag atataccatg gcgatatccc gggagctcgt
+ggatccgaat tctgtacagg cgcgcctgca ggacgtcgac ggtaccatcg atacgcgttc
+gaagcttgcg gccgcacagc tgtatacacg tgcaagccag ccagaactcg ctcctgaaga
+cccagaggat ctcgagcacc accaccacca ccactaatgt taattaagtt gggcgttgta
+atcatagtca taatcaatac tcctgactgc gttagcaatt taactgtgat aaactaccgc
+attaaagcta ttcgatgata agctgtcaaa catgataatt cttgaagacg aaagggccta
+ggctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
+cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
+tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
+tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
+ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
+aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
+cttttgttta tttttctaaa tacattcaaa tatgtatccg ctgagcaata actagcataa
+ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
+ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
+cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
+cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
+tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
+gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
+cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
+attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
+tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca
+cgatggcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
+ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
+cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
+cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
+accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
+ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
+ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
+tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
+acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
+tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
+actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
+ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
+aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
+ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
+cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
+aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
+actcatactc ttcctttttc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac
+tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
+gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
+caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
+actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
+acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
+cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
+gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
+cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
+gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
+tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
+tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
+gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
+aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
+agcgagtcag tgagcgagga agccggcgat aatggcctgc ttctcgccga aacgtttggt
+ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
+caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
+tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgac
+cggtgaattg tgagcgctca caattctcgt gacatcataa cgtcccgcga aat
-[[Category:Plasmids]]
+[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:lac Operator]][[Category:HSV Tag]][[Category:His Tag]][[Category:E.coli Promoter]][[Category:Ampicillin Marker Plasmids]]
 __NOTOC__