pETBlue-2
At a Glance
pETBlue-2, Image made with PlasMapper
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| Type |
Expression |
|---|---|
| Origin of Replication |
pUC, f1 |
| Copy # |
high |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
lac operator |
| Accession Number |
|
| edit table |
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Notes
pETBlue-2 provides a vector-encoded ATG start codon and a variety of downstream cloning sites.
History
The pETBlue™ System combines the visual identification of recombinants and high plasmid copy number, found in popular blue/white screening vectors, with the tightly controlled high yield protein expression obtained with pET vectors.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gacttacaat
61 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtacg ggcctcttcg
121 ctattacgcc agcttgcgaa cggtgggtgc gctgcaaggc gattaagttg ggtaacgcca
181 ggattctccc agtcacgacg ttgtaaaacg acggccagcg agagatcttg attggctagc
241 agaataattt tgtttaactt taagaaggag atataccatg gcgatatccc gggagctcgt
301 ggatccgaat tctgtacagg cgcgcctgca ggacgtcgac ggtaccatcg atacgcgttc
361 gaagcttgcg gccgcacagc tgtatacacg tgcaagccag ccagaactcg ctcctgaaga
421 cccagaggat ctcgagcacc accaccacca ccactaatgt taattaagtt gggcgttgta
481 atcatagtca taatcaatac tcctgactgc gttagcaatt taactgtgat aaactaccgc
541 attaaagcta ttcgatgata agctgtcaaa catgataatt cttgaagacg aaagggccta
601 ggctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
661 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
721 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
781 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
841 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
901 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
961 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctgagcaata actagcataa
1021 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
1081 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
1141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
1201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
1261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
1321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
1381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
1441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
1501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca
1561 cgatggcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
1621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
1681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
1741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
1801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
1861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
1921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
1981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
2461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
2521 actcatactc ttcctttttc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac
2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
2761 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
3001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
3301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
3361 agcgagtcag tgagcgagga agccggcgat aatggcctgc ttctcgccga aacgtttggt
3421 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
3481 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
3541 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgac
3601 cggtgaattg tgagcgctca caattctcgt gacatcataa cgtcccgcga aat
