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==At a Glance ==
==At a Glance ==
[[Image:PGPS3.JPG|thumb|right|200px|This is an image of pGPS3 from the NEB catalog]]
[[Image:PGPS3.JPG|thumb|right|400px|pGPS3, Image provided by [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pGPS3_map.pdf NEB]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="Y46d878621c804"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
cloning
Transposon Donor
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||
pUC19
pUC19
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||
similar to that of pUC19
High
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
kanamycin
Kanamycin <br>
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pgps3.txt NEB]


|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
Transprimer-1 <br>
Tn7R<ref name='PMID:9055068'/><ref name='PMID:10666445'/> <br>
Tn7L


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!align=left {{table heading style}} |Accession Number
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==Notes==
pGPS3 is the Transprimer-1 donor plasmid used in the GPS™-M Mutagenesis System (NEB #E7101S). TnsABC* Transposase removes the Transprimer-1 element from pGPS3 and inserts it randomly into the target DNA in vitro (1-3). Transprimer-1 encodes kanamycin-resistance. Isolated from E. coli ER2502, this double-stranded plasmid is 4293 bp in size.


==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
Line 44: Line 58:


==Sources==
==Sources==
pGPS3 is isolated from E. coli [[ER2502]]
[http://www.neb.com/nebecomm/products/productN7130.asp New England Biolabs]
 
==Sequence ==
      1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
      51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC
    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC
    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA
    351  GGTAATGAAA TGGCAGATCC AACATGTGAG CAAAAGGCCA GCAAAAGGCC
    401  AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
    451  CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
    501  CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
    551  CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
    601  CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
    651  GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
    701  GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
    751  CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
    801  TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
    851  CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
    901  GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
    951  AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
    1001  CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
    1051  TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
    1101  TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
    1151  AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
    1201  CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA
    1251  TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
    1301  CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
    1351  TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
    1401  GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
    1451  GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
    1501  CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT
    1551  TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
    1601  TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
    1651  GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
    1701  TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
    1751  ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
    1801  GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC
    1851  TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
    1901  CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
    1951  AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
    2001  AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
    2051  ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
    2101  CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG
    2151  ATCTATGTCG GGTGCGGAGA AAGAGGTAAT GAAATGGCAT CCGGATCTGC
    2201  ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT CTGTATTAAC
    2251  GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC
    2301  ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA
    2351  TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT
    2401  TACCCCCATG AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC
    2451  AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT TTATCAGAAG CCAGACATTA
    2501  ACGCTTCTGG AGAAACTCAA CGAGCTGGAC GCGGATGAAC AGGCAGAGCT
    2551  CTTACTGTCA TGCCATCCGT ATGTGGGCGG ACAATAAAGT CTTAAACTGA
    2601  ACAAAATAGA TCTAAACTAT GACAATAAAG TCTTAAACTA GACAGAATAG
    2651  TTGTAAACTG AAATCAGTCC AGTTATGCTG TGAAAAAGCA TACTGGACTT
    2701  TTGTTATGGC TAAAGCAAAC TCTTCATTTT CTGAAGTGCA AATTGCCCGT
    2751  CGTATTAAAG AGGGGCGTGG GGTCGACGCG GCCGCTAACT ATAACGGTCC
    2801  TAAGGTAGCG AGTTTAAACG ATATCGGATC CGGCCGCCGC TGAGGTCTGC
    2851  CTCGTGAAGA AGGTGTTGCT GACTCATACC AGGCCTGAAT CGCCCCATCA
    2901  TCCAGCCAGA AAGTGAGGGA GCCACGGTTG ATGAGAGCTT TGTTGTAGGT
    2951  GGACCAGTTG GTGATTTTGA ACTTTTGCTT TGCCACGGAA CGGTCTGCGT
    3001  TGTCGGGAAG ATGCGTGATC TGATCCTTCA ACTCAGCAAA AGTTCGATTT
    3051  ATTCAACAAA GCCGCCGTCC CGTCAAGTCA GCGTAATGCT CTGCCAGTGT
    3101  TACAACCAAT TAACCAATTC TGATTAGAAA AACTCATCGA GCATCAAATG
    3151  AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA
    3201  GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG
    3251  GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA
    3301  ACCTATTAAT TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC
    3351  CATGAGTGAC GACTGAATCC GGTGAGAATG GCAAAAGCTT ATGCATTTCT
    3401  TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT CAAAATCACT
    3451  CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA
    3501  ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC
    3551  CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG
    3601  ATATTCTTCT AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA
    3651  GTAACCATGC ATCATCAGGA GTACGGATAA AATGCTTGAT GGTCGGAAGA
    3701  GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT CTGTAACATC
    3751  ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG
    3801  GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG
    3851  CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG
    3901  CGGCCTCGAG CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG
    3951  TATTACTGTT TATGTAAGCA GACAGTTTTA TTGTTCATGA TGATATATTT
    4001  TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA CGTGGCTTAC
    4051  TAGGATCCGA TATCATTTAA ATCTAGGGAT AACAGGGTAA TACTAGTGTC
    4101  GACCAACCAG ATAAGTGAAA TCTAGTTCCA AACTATTTTG TCATTTTTAA
    4151  TTTTCGTATT AGCTTACGAC GCTACACCCA GTTCCCATCT ATTTTGTCAC
    4201  TCTTCCCTAA ATAATCCTTA AAAACTCCAT TTCCACCCCT CCCAGTTCCC
    4251  AACTATTTTG TCCGCCCACA CCGTAAGATG CTTTTCTGTG ACT


==Sequence {{HelpIcon|Help:Plasmid}}==
==References==
{{PlasmidSeq}}


==Images {{HelpIcon|Help:Plasmid}}==
<ref name='PMID: 8556868'/>
{{PlasmidImage}}


==References==
{{RefHelp}}
<references/>
<references/>
[http://www.neb.com/nebecomm/products/productN7130.asp New England biolabs]
 
__NOTOC__
__NOTOC__




[[Category:Plasmids]]
[[Category:Plasmids]][[Category:NEB Plasmids]]
[[Category:Kanamycin Marker Plasmids]]
[[Category:Ampicillin Marker Plasmids]]
[[Category:Donor Plasmids]][[Category:Tn7R]][[Category:Tn7L]][[Category:Transprimer-1]]

Latest revision as of 18:37, 17 June 2010

At a Glance

pGPS3, Image provided by NEB
Type

Transposon Donor

Origin of Replication

pUC19

Copy #

High

Markers

Kanamycin
Ampicillin

Link to Sequence

NEB

Features

Transprimer-1
Tn7R[1][2]
Tn7L

Accession Number


edit table



Notes

pGPS3 is the Transprimer-1 donor plasmid used in the GPS™-M Mutagenesis System (NEB #E7101S). TnsABC* Transposase removes the Transprimer-1 element from pGPS3 and inserts it randomly into the target DNA in vitro (1-3). Transprimer-1 encodes kanamycin-resistance. Isolated from E. coli ER2502, this double-stranded plasmid is 4293 bp in size.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

New England Biolabs

Sequence

      1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 
     51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 
    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 
    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 
    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 
    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 
    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 
    351  GGTAATGAAA TGGCAGATCC AACATGTGAG CAAAAGGCCA GCAAAAGGCC 
    401  AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
    451  CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
    501  CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 
    551  CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
    601  CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
    651  GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 
    701  GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
    751  CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
    801  TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 
    851  CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
    901  GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
    951  AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 
   1001  CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
   1051  TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
   1101  TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 
   1151  AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 
   1201  CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
   1251  TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 
   1301  CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 
   1351  TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
   1401  GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 
   1451  GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 
   1501  CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
   1551  TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 
   1601  TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 
   1651  GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
   1701  TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 
   1751  ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 
   1801  GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
   1851  TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 
   1901  CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 
   1951  AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
   2001  AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 
   2051  ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
   2101  CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
   2151  ATCTATGTCG GGTGCGGAGA AAGAGGTAAT GAAATGGCAT CCGGATCTGC 
   2201  ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT CTGTATTAAC 
   2251  GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC 
   2301  ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA 
   2351  TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT 
   2401  TACCCCCATG AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC 
   2451  AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT TTATCAGAAG CCAGACATTA 
   2501  ACGCTTCTGG AGAAACTCAA CGAGCTGGAC GCGGATGAAC AGGCAGAGCT 
   2551  CTTACTGTCA TGCCATCCGT ATGTGGGCGG ACAATAAAGT CTTAAACTGA 
   2601  ACAAAATAGA TCTAAACTAT GACAATAAAG TCTTAAACTA GACAGAATAG 
   2651  TTGTAAACTG AAATCAGTCC AGTTATGCTG TGAAAAAGCA TACTGGACTT 
   2701  TTGTTATGGC TAAAGCAAAC TCTTCATTTT CTGAAGTGCA AATTGCCCGT 
   2751  CGTATTAAAG AGGGGCGTGG GGTCGACGCG GCCGCTAACT ATAACGGTCC 
   2801  TAAGGTAGCG AGTTTAAACG ATATCGGATC CGGCCGCCGC TGAGGTCTGC 
   2851  CTCGTGAAGA AGGTGTTGCT GACTCATACC AGGCCTGAAT CGCCCCATCA 
   2901  TCCAGCCAGA AAGTGAGGGA GCCACGGTTG ATGAGAGCTT TGTTGTAGGT 
   2951  GGACCAGTTG GTGATTTTGA ACTTTTGCTT TGCCACGGAA CGGTCTGCGT 
   3001  TGTCGGGAAG ATGCGTGATC TGATCCTTCA ACTCAGCAAA AGTTCGATTT 
   3051  ATTCAACAAA GCCGCCGTCC CGTCAAGTCA GCGTAATGCT CTGCCAGTGT 
   3101  TACAACCAAT TAACCAATTC TGATTAGAAA AACTCATCGA GCATCAAATG 
   3151  AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA 
   3201  GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG 
   3251  GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA 
   3301  ACCTATTAAT TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC 
   3351  CATGAGTGAC GACTGAATCC GGTGAGAATG GCAAAAGCTT ATGCATTTCT 
   3401  TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT CAAAATCACT 
   3451  CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA 
   3501  ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC 
   3551  CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG 
   3601  ATATTCTTCT AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA 
   3651  GTAACCATGC ATCATCAGGA GTACGGATAA AATGCTTGAT GGTCGGAAGA 
   3701  GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT CTGTAACATC
   3751  ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG 
   3801  GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG 
   3851  CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG 
   3901  CGGCCTCGAG CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG 
   3951  TATTACTGTT TATGTAAGCA GACAGTTTTA TTGTTCATGA TGATATATTT 
   4001  TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA CGTGGCTTAC 
   4051  TAGGATCCGA TATCATTTAA ATCTAGGGAT AACAGGGTAA TACTAGTGTC 
   4101  GACCAACCAG ATAAGTGAAA TCTAGTTCCA AACTATTTTG TCATTTTTAA 
   4151  TTTTCGTATT AGCTTACGAC GCTACACCCA GTTCCCATCT ATTTTGTCAC 
   4201  TCTTCCCTAA ATAATCCTTA AAAACTCCAT TTCCACCCCT CCCAGTTCCC 
   4251  AACTATTTTG TCCGCCCACA CCGTAAGATG CTTTTCTGTG ACT

References

[3]

  1. ↑ Stellwagen, AE & Craig, NL (1997) Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145 573-85 PubMed
  2. ↑ Biery, MC et al. (2000) A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucleic Acids Res. 28 1067-77 PubMed
  3. ↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
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