pGPS3
At a Glance
pGPS3, Image provided by NEB
<protect>
| Type |
Transposon Donor |
|---|---|
| Origin of Replication |
pUC19 |
| Copy # |
High |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features | |
| Accession Number |
|
| edit table |
</protect>
Notes
pGPS3 is the Transprimer-1 donor plasmid used in the GPS™-M Mutagenesis System (NEB #E7101S). TnsABC* Transposase removes the Transprimer-1 element from pGPS3 and inserts it randomly into the target DNA in vitro (1-3). Transprimer-1 encodes kanamycin-resistance. Isolated from E. coli ER2502, this double-stranded plasmid is 4293 bp in size.
History
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Sources
Sequence
1 GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
51 TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC
101 CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
151 GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
201 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC
251 ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
301 CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA
351 GGTAATGAAA TGGCAGATCC AACATGTGAG CAAAAGGCCA GCAAAAGGCC
401 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
451 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
501 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
551 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
601 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
651 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
701 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
751 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
801 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
851 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
901 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
951 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
1001 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
1051 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
1101 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
1151 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
1201 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA
1251 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
1301 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
1351 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
1401 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
1451 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
1501 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT
1551 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
1601 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
1651 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
1701 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
1751 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
1801 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC
1851 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
1901 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
1951 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
2001 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
2051 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
2101 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG
2151 ATCTATGTCG GGTGCGGAGA AAGAGGTAAT GAAATGGCAT CCGGATCTGC
2201 ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT CTGTATTAAC
2251 GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC
2301 ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA
2351 TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT
2401 TACCCCCATG AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC
2451 AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT TTATCAGAAG CCAGACATTA
2501 ACGCTTCTGG AGAAACTCAA CGAGCTGGAC GCGGATGAAC AGGCAGAGCT
2551 CTTACTGTCA TGCCATCCGT ATGTGGGCGG ACAATAAAGT CTTAAACTGA
2601 ACAAAATAGA TCTAAACTAT GACAATAAAG TCTTAAACTA GACAGAATAG
2651 TTGTAAACTG AAATCAGTCC AGTTATGCTG TGAAAAAGCA TACTGGACTT
2701 TTGTTATGGC TAAAGCAAAC TCTTCATTTT CTGAAGTGCA AATTGCCCGT
2751 CGTATTAAAG AGGGGCGTGG GGTCGACGCG GCCGCTAACT ATAACGGTCC
2801 TAAGGTAGCG AGTTTAAACG ATATCGGATC CGGCCGCCGC TGAGGTCTGC
2851 CTCGTGAAGA AGGTGTTGCT GACTCATACC AGGCCTGAAT CGCCCCATCA
2901 TCCAGCCAGA AAGTGAGGGA GCCACGGTTG ATGAGAGCTT TGTTGTAGGT
2951 GGACCAGTTG GTGATTTTGA ACTTTTGCTT TGCCACGGAA CGGTCTGCGT
3001 TGTCGGGAAG ATGCGTGATC TGATCCTTCA ACTCAGCAAA AGTTCGATTT
3051 ATTCAACAAA GCCGCCGTCC CGTCAAGTCA GCGTAATGCT CTGCCAGTGT
3101 TACAACCAAT TAACCAATTC TGATTAGAAA AACTCATCGA GCATCAAATG
3151 AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA
3201 GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG
3251 GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA
3301 ACCTATTAAT TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC
3351 CATGAGTGAC GACTGAATCC GGTGAGAATG GCAAAAGCTT ATGCATTTCT
3401 TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT CAAAATCACT
3451 CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA
3501 ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC
3551 CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG
3601 ATATTCTTCT AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA
3651 GTAACCATGC ATCATCAGGA GTACGGATAA AATGCTTGAT GGTCGGAAGA
3701 GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT CTGTAACATC
3751 ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG
3801 GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG
3851 CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG
3901 CGGCCTCGAG CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG
3951 TATTACTGTT TATGTAAGCA GACAGTTTTA TTGTTCATGA TGATATATTT
4001 TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA CGTGGCTTAC
4051 TAGGATCCGA TATCATTTAA ATCTAGGGAT AACAGGGTAA TACTAGTGTC
4101 GACCAACCAG ATAAGTGAAA TCTAGTTCCA AACTATTTTG TCATTTTTAA
4151 TTTTCGTATT AGCTTACGAC GCTACACCCA GTTCCCATCT ATTTTGTCAC
4201 TCTTCCCTAA ATAATCCTTA AAAACTCCAT TTCCACCCCT CCCAGTTCCC
4251 AACTATTTTG TCCGCCCACA CCGTAAGATG CTTTTCTGTG ACT
References
- ↑ Stellwagen, AE & Craig, NL (1997) Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145 573-85 PubMed
- ↑ Biery, MC et al. (2000) A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucleic Acids Res. 28 1067-77 PubMed
- ↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
