View source for pKO500

==At a Glance {{HelpIcon|Help:Plasmid}}==
[[File:PKO500.jpg|thumb|450px|pKO500, Image made with [[PlasMapper]]]]
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{| {{Prettytable}} width='30%' align=left  id="I46b744e5c84f2"  class=" tableEdit PlasmidTable"  

|-
!align=left {{table heading style}} |Type
||
promoter cloning vector
|-
!align=left {{table heading style}} |Origin of Replication
||

|-
!align=left {{table heading style}} |Copy #
||
medium copy number
has the origin and copy number control genes of pBR322
|-
!align=left {{table heading style}} |Markers
||
''bla'' gene from pBR322 (AmpR)
promoterless ''galK'' from ''E. coli''
|-
!align=left {{table heading style}} |Link to Sequence
||

|-
!align=left {{table heading style}} |Features
||
PMID: 2164010
|-
!align=left {{table heading style}} |Accession Number
||


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==History {{HelpIcon|Help:Plasmid}}==
The contruction of pKO500 is described in Wickner et al. (1990)<ref name=PMID:2164010/>.   

"[pKO500] was constructed from PKO-1, which contains the ''bla'' gene of pBR322 and the ''galK'' gene of ''E. coli'' (23)<ref name=PMID:6101056/>.  A 310-bp PvuII fragment containing the P''lac'' promoter, polylinker site, and ''lacZ'' sequences complementary to the universal sequencing primers of M13mp11 (24)<ref name=PMID:6295880/> was cloned into the ''Sma''I site of pKO-1.  The orientation was such that the P''lac'' promoter expressed the ''galK'' gene.  From the resultant plasmid, pKL500, the sequences containing the P''lac'' promoter were deleted by digestion with ''Hin''dIII (which cuts in pKO-1 and the mpll polylinker sequences).  The DNA was ligated to create the plasmid vector pKO500."

==Sources {{HelpIcon|Help:Plasmid}}==
{{PlasmidSources}}

==Sequence {{HelpIcon|Help:Plasmid}}==
The sequence of pKO500 was assembled from the GenBank accessions of pKO1 (L08923) and M13mp11 (L08820).  
One apparent discrepancy with the description above is the size of the PvuII fragment from M13mp11.  Based on accession L08820, this fragment is 409 bp.

<dna_display>
ttctggcgaatcctctgaccagccagaaaacgacctttctgtggtgaaaccggatgctgcaattcagagcgccagcaagt
gggggacagcagaagacctgaccgccgcagagtggatgtttgacatggtgaagactatcgcaccatcagccagaaaaccg
aattttgctgggtgggctaacgatatccgcctgatgcgtgaacgtgacggacgtaaccaccgcgacatgtgtgtgctgtt
ccgctgggcatgccaggacaacttctggtccggtaacgtgctgagcccggccaagcttgggctgcaggtcgactctagag
gatccccgggcgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaa
tcgccttgcagcacatccccctttcgccaggggcaataagggctgcacgcgcacttttatccgcctctgctgcgctccgc
caccgtacgtaaatttatggttggttatgaaatgctggcagagacccagcgagacctgaccgcagaacaggcagcagagc
gtttgcgcgcagtcagcgatatccattttcgcgaatccggagtgtaagaaatgagtctgaaagaaaaaacacaatctctg
tttgccaacgcatttggctaccctgccactcacaccattcaggcgcctggccgcgtgaatttgattggtgaacacaccga
ctacaacgacggtttcgttctgccctgcgcgattgattatcaaaccgtgatcagttgtgcaccacgcgatgaccgtaaag
ttcgcgtgatggcagccgattatgaaaatcagctcgacgagttttccctcgatgcgcccattgtcgcacatgaaaactat
caatgggctaactacgttcgtggcgtggtgaaacatctgcaactgcgtaacaacagcttcggcggcgtggacatggtgat
cagcggcaatgtgccgacgggtgccgggttaagttcttccgcttcactggaagtcgcggtcggaaccgtattgcagcagc
tttatcatctgccgctggacggcgcacaaatcgcgcttaacggtcaggaagcagaaaaccagtttgtaggctgtaactgc
gggatcatggatcagctaatttccgcgctcggcaagaaagatcatgccttgctgatcgattgccgctcactggggaccaa
agcagtttccatgcccaaaggtgtggctgtcgtcatcatcaacagtaacttcaaacgtaccctggttggcagcgaataca
acacccgtcgtgaacagtgcgaaaccggtgcgcgtttcttccagcagccagccctgcgtgatgtcaccattgaagagttc
aacgctgttgcgcatgaactggacccgatcgtggcaaaacgcgtgcgtcatatactgactgaaaacgcccgcaccgttga
agctgccagcgcgctggagcaaggcgacctgaaacgtatgggcgagttgatggcggagtctcatgcctctatgcgcgatg
atttcgaaatcaccgtgccgcaaattgacactctggtagaaatcgtcaaagctgtgattggcgacaaaggtggcgtacgc
atgaccggcggcggatttggcggctgtatcgtcgcgctgatcccggaagagctggtgcctgccgcacagcaagctgtcgc
tgaacaatatgaagcaaaaacaggtattaaagagactttttacgtttgtaaaccatcacaaggagcaggacagtgctgaa
cgaaactcccgcactggcacccgatggcagccgtaccgactgttctgcctcgcgcgtttcggtgatgacggtgaaaacct
ctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgt
cagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatg
cggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgc
atcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactc
aaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca
ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctca
agtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgt
tccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgta
ggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcc
ttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggat
tagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtat
ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgct
ggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc
tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacct
agatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc
ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataac
tacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat
cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaat
tgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggt
gtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgt
gcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatg
gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactt
taaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatg
taacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca
aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagca
tttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca
tttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgag
gccctttcgtcttcaagaa
</dna_display>

==Images {{HelpIcon|Help:Plasmid}}==
{{PlasmidImage}}

==References==
{{RefHelp}}
<references/>

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[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]]