pKO500
At a Glance
<protect>
| Type |
promoter cloning vector |
|---|---|
| Origin of Replication | |
| Copy # |
medium copy number has the origin and copy number control genes of pBR322 |
| Markers |
bla gene from pBR322 (AmpR) promoterless galK from E. coli |
| Link to Sequence | |
| Features | |
| Accession Number |
|
| edit table |
</protect>
History
The contruction of pKO500 is described in Wickner et al. (1990)[1].
"[pKO500] was constructed from PKO-1, which contains the bla gene of pBR322 and the galK gene of E. coli (23)[2]. A 310-bp PvuII fragment containing the Plac promoter, polylinker site, and lacZ sequences complementary to the universal sequencing primers of M13mp11 (24)[3] was cloned into the SmaI site of pKO-1. The orientation was such that the Plac promoter expressed the galK gene. From the resultant plasmid, pKL500, the sequences containing the Plac promoter were deleted by digestion with HindIII (which cuts in pKO-1 and the mpll polylinker sequences). The DNA was ligated to create the plasmid vector pKO500."
Sources
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Sequence
The sequence of pKO500 was assembled from the GenBank accessions of pKO1 (L08923) and M13mp11 (L08820). One apparent discrepancy with the description above is the size of the PvuII fragment from M13mp11. Based on accession L08820, this fragment is 409 bp.
<dna_display> ttctggcgaatcctctgaccagccagaaaacgacctttctgtggtgaaaccggatgctgcaattcagagcgccagcaagt gggggacagcagaagacctgaccgccgcagagtggatgtttgacatggtgaagactatcgcaccatcagccagaaaaccg aattttgctgggtgggctaacgatatccgcctgatgcgtgaacgtgacggacgtaaccaccgcgacatgtgtgtgctgtt ccgctgggcatgccaggacaacttctggtccggtaacgtgctgagcccggccaagcttgggctgcaggtcgactctagag gatccccgggcgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaa tcgccttgcagcacatccccctttcgccaggggcaataagggctgcacgcgcacttttatccgcctctgctgcgctccgc caccgtacgtaaatttatggttggttatgaaatgctggcagagacccagcgagacctgaccgcagaacaggcagcagagc gtttgcgcgcagtcagcgatatccattttcgcgaatccggagtgtaagaaatgagtctgaaagaaaaaacacaatctctg tttgccaacgcatttggctaccctgccactcacaccattcaggcgcctggccgcgtgaatttgattggtgaacacaccga ctacaacgacggtttcgttctgccctgcgcgattgattatcaaaccgtgatcagttgtgcaccacgcgatgaccgtaaag ttcgcgtgatggcagccgattatgaaaatcagctcgacgagttttccctcgatgcgcccattgtcgcacatgaaaactat caatgggctaactacgttcgtggcgtggtgaaacatctgcaactgcgtaacaacagcttcggcggcgtggacatggtgat cagcggcaatgtgccgacgggtgccgggttaagttcttccgcttcactggaagtcgcggtcggaaccgtattgcagcagc tttatcatctgccgctggacggcgcacaaatcgcgcttaacggtcaggaagcagaaaaccagtttgtaggctgtaactgc gggatcatggatcagctaatttccgcgctcggcaagaaagatcatgccttgctgatcgattgccgctcactggggaccaa agcagtttccatgcccaaaggtgtggctgtcgtcatcatcaacagtaacttcaaacgtaccctggttggcagcgaataca acacccgtcgtgaacagtgcgaaaccggtgcgcgtttcttccagcagccagccctgcgtgatgtcaccattgaagagttc aacgctgttgcgcatgaactggacccgatcgtggcaaaacgcgtgcgtcatatactgactgaaaacgcccgcaccgttga agctgccagcgcgctggagcaaggcgacctgaaacgtatgggcgagttgatggcggagtctcatgcctctatgcgcgatg atttcgaaatcaccgtgccgcaaattgacactctggtagaaatcgtcaaagctgtgattggcgacaaaggtggcgtacgc atgaccggcggcggatttggcggctgtatcgtcgcgctgatcccggaagagctggtgcctgccgcacagcaagctgtcgc tgaacaatatgaagcaaaaacaggtattaaagagactttttacgtttgtaaaccatcacaaggagcaggacagtgctgaa cgaaactcccgcactggcacccgatggcagccgtaccgactgttctgcctcgcgcgtttcggtgatgacggtgaaaacct ctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgt cagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatg cggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgc atcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactc aaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctca agtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgt tccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgta ggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcc ttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggat tagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtat ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgct ggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacct agatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataac tacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaat tgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggt gtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgt gcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatg gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactt taaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatg taacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagca tttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca tttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgag gccctttcgtcttcaagaa </dna_display>
Images
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Wickner, S et al. (1990) Deletion analysis of the mini-P1 plasmid origin of replication and the role of Escherichia coli DnaA protein. J. Biol. Chem. 265 11622-7 PubMed
- ↑ McKenney, K et al. (1981) A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif Anal 2 383-415 PubMed
- ↑ Messing, J & Vieira, J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19 269-76 PubMed
