View source for pKLAC1

==At a Glance==
[[Image:PKLAC1_Vector.JPG|thumb|right|400px|pkLAC1, Image provided by [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pklac1_map.pdf NEB]]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.R46e6d2bf5f55f-->
<!--
******************************************************************************************
* 
*   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. ** 
* 
****************************************************************************************** -->
{| {{Prettytable}} width='30%' align=left  id="R46e6d2bf5f55f"  class=" tableEdit PlasmidTable"  

|-
!align=left {{table heading style}} |Type
||
Expression
|-
!align=left {{table heading style}} |Origin of Replication
||
pMB1
|-
!align=left {{table heading style}} |Copy #
||

|-
!align=left {{table heading style}} |Markers
||
Ampicillin, Acetamidase<ref name='PMID:16630278'/>
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pklac1.txt NEB]
|-
!align=left {{table heading style}} |Features
||
α-mating factor<br />
MCS<br />
AdH1 promoter region<br />
amdS<ref name='PMID:16630278'/><br />
LAC4 promoter<ref name='PMID:16269745'/>
|-
!align=left {{table heading style}} |Accession Number
||


|- class="tableEdit_footer" 
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R46e6d2bf5f55f&page=112442&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.R46e6d2bf5f55f--></protect>
<BR>
<Br clear='all'>

==Notes==
pKLAC1 can be used for secreted protein expression or intracellular protein expression.
A standard DNA purification procedure is used to derive pKLAC1 from E. coli strain ER2268.
LAC4 promoter is not expressed in E. coli. Toxic genes can be cloned before their expression in yeast.

==History==
pKLAC1 was designed as an expression vector that can replicate in E.coli and capable of stable integration in to the genome of the yeast K.lactis <ref name='PMID:16269745'/>.

==Sources==
[http://www.neb.com/nebecomm/products/productN3740.asp New England Biolabs]

==References==
{{BacktoTop}}
{{RefHelp}}
<references />
Colussi, P.A. and Taron, C.H., unpublished observations.
==Sequence==
       1  AAGCTTGAAA AAAATGAAAT TCTCTACTAT ATTAGCCGCA TCTACTGCTT 
      51  TAATTTCCGT TGTTATGGCT GCTCCAGTTT CTACCGAAAC TGACATCGAC 
     101  GATCTTCCAA TATCGGTTCC AGAAGAAGCC TTGATTGGAT TCATTGACTT 
     151  AACCGGGGAT GAAGTTTCCT TGTTGCCTGT TAATAACGGA ACCCACACTG 
     201  GTATTCTATT CTTAAACACC ACCATCGCTG AAGCTGCTTT CGCTGACAAG 
     251  GATGATCTCG AGAAAAGAGA GGCTGAAGCT AGAAGAGCTA GATCTCCTAG 
     301  GGGTACCGTC GACGGCGCGC CTGCGGCCGC TTAATTAAGG CCTTGAATCG 
     351  AGAATTTATA CTTAGATAAG TATGTACTTA CAGGTATATT TCTATGAGAT 
     401  ACTGATGTAT ACATGCATGA TAATATTTAA ACGGTTATTA GTGCCGATTG 
     451  TCTTGTGCGA TAATGACGTT CCTATCAAAG CAATACACTT ACCACCTATT 
     501  ACATGGGCCA AGAAAATATT TTCGAACTTG TTTAGAATAT TAGCACAGAG 
     551  TATATGATGA TATCCGTTAG ATTATGCATG ATTCATTCCT ACAACTTTTT 
     601  CGTAGCATAA GGATTAATTA CTTGGATGCC AATAAAAAAA AAAAACATCG 
     651  AGAAAATTTC AGCATGCTCA GAAACAATTG CAGTGTATCA AAGTAAAAAA 
     701  AAGATTTTCA CTACATGTTC CTTTTGAAGA AAGAAAATCA TGGAACATTA 
     751  GATTTACAAA AATTTAACCA CCGCTGATTA ACGATTAGAC CGTTAAGCGC 
     801  ACAACAGGTT ATTAGTACAG AGAAAGCATT CTGTGGTGTT GCCCCGGACT 
     851  TTCTTTTGCG ACATAGGTAA ATCGAATACC ATCATACTAT CTTTTCCAAT 
     901  GACTCCCTAA AGAAAGACTC TTCTTCGATG TTGTATACGT TGGAGCATAG 
     951  GGCAAGAATT GTGGCTTGAG ATCATCCTTT TGTTGTTTCC GGGTGTACAA 
    1001  TATGGACTTC CTCTTTTCTG GCAACCAAAC CCATACATCG GGATTCCTAT 
    1051  AATACCTTCG TTGGTCTCCC TAACATGTAG GTGGCGGAGG GGAGATATAC 
    1101  AATAGAACAG ATACCAGACA AGACATAATG GGCTAAACAA GACTACACCA 
    1151  ATTACACTGC CTCATTGATG GTGGTACATA ACGAACTAAT ACTGTAGCCC 
    1201  TAGACTTGAT AGCCATCATC ATATCGAAGT TTCACTACCC TTTTTCCATT 
    1251  TGCCATCTAT TGAAGTAATA ATAGGCGCAT GCAACTTCTT TTCTTTTTTT 
    1301  TTCTTTTCTC TCTCCCCCGT TGTTGTCTCA CCATATCCGC AATGACAAAA 
    1351  AAATGATGGA AGACACTAAA GGAAAAAATT AACGACAAAG ACAGCACCAA 
    1401  CAGATGTCGT TGTTCCAGAG CTGATGAGGG GTATCTCGAA GCACACGAAA 
    1451  CTTTTTCCTT CCTTCATTCA CGCACACTAC TCTCTAATGA GCAACGGTAT 
    1501  ACGGCCTTCC TTCCAGTTAC TTGAATTTGA AATAAAAAAA AGTTTGCTGT 
    1551  CTTGCTATCA AGTATAAATA GACCTGCAAT TATTAATCTT TTGTTTCCTC 
    1601  GTCATTGTTC TCGTTCCCTT TCTTCCTTGT TTCTTTTTCT GCACAATATT 
    1651  TCAAGCTATA CCAAGCATAC AATCAAGGAA TTCCGGATCC GCCACCATGC 
    1701  CTCAATCCTG GGAAGAACTG GCCGCTGATA AGCGCGCCCG CCTCGCAAAA 
    1751  ACCATCCCTG ATGAATGGAA AGTCCAGACG CTGCCTGCGG AAGACAGCGT 
    1801  TATTGATTTC CCAAAGAAAT CGGGGATCCT TTCAGAGGCC GAACTGAAGA 
    1851  TCACAGAGGC TTCCGCTGCG GATCTTGTGT CCAAGCTGGC GGCCGGAGAG 
    1901  TTGACCTCGG TGGAAGTTAC GCTAGCATTC TGTAAACGGG CAGCAATCGC 
    1951  CCAGCAGTTA ACAAACTGCG CCCACGAGTT CTTCCCTGAC GCCGCTCTCG 
    2001  CGCAGGCAAG GGAACTCGAT GAATACTACG CAAAGCACAA GAGACCCGTT 
    2051  GGTCCACTCC ATGGCCTCCC CATCTCTCTC AAAGACCAGC TTCGAGTCAA 
    2101  GGGCTACGAA ACATCAATGG GCTACATCTC ATGGCTAAAC AAGTACGACG 
    2151  AAGGGGACTC GGTTCTGACA ACCATGCTCC GCAAAGCCGG TGCCGTCTTC 
    2201  TACGTCAAGA CCTCTGTCCC GCAGACCCTG ATGGTCTGCG AGACAGTCAA 
    2251  CAACATCATC GGGCGCACCG TCAACCCACG CAACAAGAAC TGGTCGTGCG 
    2301  GCGGCAGTTC TGGTGGTGAG GGTGCGATCG TTGGGATTCG TGGTGGCGTC 
    2351  ATCGGTGTAG GAACGGATAT CGGTGGCTCG ATTCGAGTGC CGGCCGCGTT 
    2401  CAACTTCCTG TACGGTCTAA GGCCGAGTCA TGGGCGGCTG CCGTATGCAA 
    2451  AGATGGCGAA CAGCATGGAG GGTCAGGAGA CGGTGCACAG CGTTGTCGGG 
    2501  CCGATTACGC ACTCTGTTGA GGACCTCCGC CTCTTCACCA AATCCGTCCT 
    2551  CGGTCAGGAG CCATGGAAAT ACGACTCCAA GGTCATCCCC ATGCCCTGGC 
    2601  GCCAGTCCGA GTCGGACATT ATTGCCTCCA AGATCAAGAA CGGCGGGCTC 
    2651  AATATCGGCT ACTACAACTT CGACGGCAAT GTCCTTCCAC ACCCTCCTAT 
    2701  CCTGCGCGGC GTGGAAACTA CCGTCGCCGC ACTCGCCAAA GCCGGTCACA 
    2751  CCGTGACCCC GTGGACGCCA TACAAGCACG ATTTCGGCCA CGATCTCATC 
    2801  TCCCATATCT ACGCGGCTGA CGGCAGCGCC GACGTAATGC GCGATATCAG 
    2851  TGCATCCGGC GAGCCGGCGA TTCCAAATAT CAAAGACCTA CTGAACCCGA 
    2901  ACATCAAAGC TGTTAACATG AACGAGCTCT GGGACACGCA TCTCCAGAAG 
    2951  TGGAATTACC AGATGGAGTA CCTTGAGAAA TGGCGGGAGG CTGAAGAAAA 
    3001  GGCCGGGAAG GAACTGGACG CCATCATCGC GCCGATTACG CCTACCGCTG 
    3051  CGGTACGGCA TGACCAGTTC CGGTACTATG GGTATGCCTC TGTGATCAAC 
    3101  CTGCTGGATT TCACGAGCGT GGTTGTTCCG GTTACCTTTG CGGATAAGAA 
    3151  CATCGATAAG AAGAATGAGA GTTTCAAGGC GGTTAGTGAG CTTGATGCCC 
    3201  TCGTGCAGGA AGAGTATGAT CCGGAGGCGT ACCATGGGGC ACCGGTTGCA 
    3251  GTGCAGGTTA TCGGACGGAG ACTCAGTGAA GAGAGGACGT TGGCGATTGC 
    3301  AGAGGAAGTG GGGAAGTTGC TGGGAAATGT GGTGACTCCA TAGCCCGGGG 
    3351  GGGGCTCGAT CCCCTCGCGA GTTGGTTCAG CTGCTGCCTG AGGCTGGACG 
    3401  ACCTCGCGGA GTTCTACCGG CAGTGCAAAT CCGTCGGCAT CCAGGAAACC 
    3451  AGCAGCGGCT ATCCGCGCAT CCATGCCCCC GAACTGCAGG AGTGGGGAGG 
    3501  CACGATGGCC GCTTTGGTCG ATCTAGATTA CGTGGAAGAA AGGTAGTAAA 
    3551  AGTAGTAGTA TAAGTAGTAA AAAGAGGTAA AAAGAGAAAA CCGGCTACAT 
    3601  ACTAGAGAAG CACGTACACA AAAACTCATA GGCACTTCAT CATACGACAG 
    3651  TTTCTTGATG CATTATAATA GTGTATTAGA TATTTTCAGA AATATGCATA 
    3701  GAACCTCCTC TTGCCTTTAC TTTTTATACA TAGAACATTG GCAGATTTAC 
    3751  TTACACTACT TTGTTTCTAC GCCATTTCTT TTGTTTTCAA CACTTAGACA 
    3801  AGTTGTTGAG AACCGGACTA CTAAAAAGCA ATGTTCCCAC TGAAAATCAT 
    3851  GTACCTGCAG GATAATAACC CCCTAATTCT GCATCGATCC AGTATGTTTT 
    3901  TTTTTCTCTA CTCATTTTTA CCTGAAGATA GAGCTTCTAA AACAAAAAAA 
    3951  ATCAGTGATT ACATGCATAT TGTGTGTTCT AGTAACCAAA GGAAAGGAAC 
    4001  AGATAGATAA AATTCCGAGA CTGTCAAATT AGGTTTTTTT CTTTTTTTTT 
    4051  GGCGGGAGTC AGTGGGCCGA AATATGTTCT TGGCCTAGAA CTTAATCTGG 
    4101  TTTGATCATG CCAATACTTG CCTGAGTGCC CGACTTTTTG CCCACCCTCT 
    4151  TGCCTTCTGT CATCCTTCAA AACCCACCTG TTTTCCAGCC GTATCTTCGC 
    4201  TCGCATCTAC ACATACTGTG CCATATCTTG TGTGTAGCCG GACGTGACTA 
    4251  TGACCAAAAA CAAACAAGGA GAACTGTTCG CCGATTTGTA ACACTCCTGC 
    4301  ATCCATCCAA GTGGGTATGC GCTATGCAAT GTTAAGCTAG GTCAGGTCAG 
    4351  ACCAGGTCCA AGGACAGCAA CTTGACTGTA TGCAACCTTT ACCATCTTTG 
    4401  CACAGAACAT ACTTGTAGCT AGCTAGTTAC ACTTATGGAC CGAAAAGGCA 
    4451  CCCCACCATG TCTGTCCGGC TTTAGAGTAC GGCCGCAGAC CGCTGATTTG 
    4501  CCTTGCCAAG CAGTAGTCAC AATGCATCGC ATGAGCACAC GGGCACGGGC 
    4551  ACGGGCACAG GAACCATTGG CAAAAATACC AGATACACTA TACCGACGTA 
    4601  TATCAAGCCC AAGTTTAAAA TTCCTAAATT TCCGCGGCTA CTTTTCAATT 
    4651  CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG 
    4701  TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 
    4751  ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 
    4801  TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
    4851  TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 
    4901  CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 
    4951  GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
    5001  AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 
    5051  GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
    5101  CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
    5151  ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 
    5201  GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
    5251  TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
    5301  TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 
    5351  CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
    5401  CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
    5451  GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 
    5501  TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
    5551  GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
    5601  AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
    5651  ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
    5701  GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
    5751  GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 
    5801  TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 
    5851  ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
    5901  CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 
    5951  TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 
    6001  GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
    6051  GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 
    6101  CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 
    6151  TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
    6201  CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 
    6251  TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 
    6301  TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
    6351  CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 
    6401  TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 
    6451  CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
    6501  TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 
    6551  CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 
    6601  TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
    6651  GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 
    6701  CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 
    6751  TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
    6801  GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
    6851  GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 
    6901  ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
    6951  TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
    7001  TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 
    7051  TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
    7101  GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
    7151  ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 
    7201  GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
    7251  AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC 
    7301  CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 
    7351  CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG 
    7401  GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG 
    7451  CCAAGCTCCC GCGGGGATCG ACTCATAAAA TAGTAACCTT CTAATGCGTA 
    7501  TCTATTGACT ACCAACCATT AGTGTGGTTG CAGAAGGCGG AATTCTCCCT 
    7551  TCTTCGAATT CAGCTTGCTT TTTCATTTTT TATTTTCCAT TTTTCAGTTT 
    7601  TTGTTTGTGT CGAATTTAGC CAGTTGCTTC TCCAAGATGA AAAAAACCCC 
    7651  TGCGCAGTTT CTGTGCTGCA AGATCCTAAT CGACTTTTCC ACCCCCCACA 
    7701  AAAGTAAATG TTCTTTTGTT ACATTCGCGT GGGTAGCTAG CTCCCCGAAT 
    7751  CTTCAAAGGA CTTAGGGACT GCACTACATC AGAGTGTGTT CACCTGGTTT 
    7801  GCTGCCTGGT TTGAAAGAAA AGAGCAGGGA ACTCGCGGGT TCCCGGCGAA 
    7851  TAATCATGCG ATAGTCCTTT GGCCTTCCAA GTCGCATGTA GAGTAGACAA 
    7901  CAGACAGGGA GGGCAGGAAG GATCTTTCAC TGAGATCCTG TATCTTGTTG 
    7951  GGTAAGTCGG ATGAAAGGGG AATCGTATGA GATTGGAGAG GATGCGGAAG 
    8001  AGGTAACGCC TTTTGTTAAC TTGTTTAATT ATTATGGGGC AGGCGAGAGG 
    8051  GGGAGGAATG TATGTGTGTG AGGCGGGCGA GACGGAGCCA TCCAGGCCAG 
    8101  GTAGAAATAG AGAAAGCCGA ATGTTAGACA ATATGGCAGC GTAGTAGAGT 
    8151  AGGTAGGTAG GCAAGTACTG CTAGCAAAGA GGAGAAGGGT AAGCTCACTC 
    8201  TTCGCATTCC ACACCGTTAG TGTGTCAGTT TGAACAAAAA AACAATCATC 
    8251  ATACCAATTG ATGGACTGTG GACTGGCTTT TGGAACGGCT TTTCGGACTG 
    8301  CGATTATTCG TGAGGAATCA AGGTAGGAAT TTGGTCATAT TTACGGACAA 
    8351  CAGTGGGTGA TTCCCATATG GAGTAGGAAA ACGAGATCAT GGTATCCTCA 
    8401  GATATGTTGC GGAATTCTGT TCACCGCAAA GTTCAGGGTG CTCTGGTGGG 
    8451  TTTCGGTTGG TCTTTGCTTT GCTTCTCCCT TGTCTTGCAT GTTAATAATA 
    8501  GCCTAGCCTG TGAGCCGAAA CTTAGGGTAG GCTTAGTGTT GGAACGTACA 
    8551  TATGTATCAC GTTGACTTGG TTTAACCAGG CGACCTGGTA GCCAGCCATA 
    8601  CCCACACACG TTTTTTGTAT CTTCAGTATA GTTGTGAAAA GTGTAGCGGA 
    8651  AATTTGTGGT CCGAGCAACA GCGTCTTTTT CTAGTAGTGC GGTCGGTTAC 
    8701  TTGGTTGACA TTGGTATTTG GACTTTGTTG CTACACCATT CACTACTTGA 
    8751  AGTCGAGTGT GAAGGGTATG ATTTCTAGTG GTGAACACCT TTAGTTACGT 
    8801  AATGTTTTCA TTGCTGTTTT ACTTGAGATT TCGATTGAGA AAAAGGTATT 
    8851  TAATAGCTCG AATCAATGTG AGAACAGAGA GAAGATGTTC TTCCCTAACT 
    8901  CGAAAGGTAT ATGAGGCTTG TGTTTCTTAG GAGAATTATT ATTCTTTTGT 
    8951  TATGTTGCGC TTGTAGTTGG AAAAGGTGAA GAGACAAAAG CTGGAATTGT 
    9001  GAGCGGATAA CAAGCTCAAC ACTTGAAATT TAGGAAAGAG CAGAATTTGG 
    9051  CAAAAAAAAT AAAAAAAAAA TAAACACACA TACTCATCGA G

[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:NEB Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Acetamidase Marker Plasmids]][[Category:α-mating factor]][[Category:MCS]][[Category:LAC4 promoter]][[Category:AdH1 promoter region]]


__NOTOC__