pKLAC1
At a Glance
pkLAC1, Image provided by NEB
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
pMB1 |
| Copy # | |
| Markers |
Ampicillin, Acetamidase[1] |
| Link to Sequence | |
| Features |
α-mating factor |
| Accession Number |
|
| edit table |
</protect>
Notes
pKLAC1 can be used for secreted protein expression or intracellular protein expression. A standard DNA purification procedure is used to derive pKLAC1 from E. coli strain ER2268. LAC4 promoter is not expressed in E. coli. Toxic genes can be cloned before their expression in yeast.
History
pKLAC1 was designed as an expression vector that can replicate in E.coli and capable of stable integration in to the genome of the yeast K.lactis [2].
Sources
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 van Ooyen, AJ et al. (2006) Heterologous protein production in the yeast Kluyveromyces lactis. FEMS Yeast Res. 6 381-92 PubMed
- ↑ 2.0 2.1 Colussi, PA & Taron, CH (2005) Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis. Appl. Environ. Microbiol. 71 7092-8 PubMed
Colussi, P.A. and Taron, C.H., unpublished observations.
Sequence
1 AAGCTTGAAA AAAATGAAAT TCTCTACTAT ATTAGCCGCA TCTACTGCTT
51 TAATTTCCGT TGTTATGGCT GCTCCAGTTT CTACCGAAAC TGACATCGAC
101 GATCTTCCAA TATCGGTTCC AGAAGAAGCC TTGATTGGAT TCATTGACTT
151 AACCGGGGAT GAAGTTTCCT TGTTGCCTGT TAATAACGGA ACCCACACTG
201 GTATTCTATT CTTAAACACC ACCATCGCTG AAGCTGCTTT CGCTGACAAG
251 GATGATCTCG AGAAAAGAGA GGCTGAAGCT AGAAGAGCTA GATCTCCTAG
301 GGGTACCGTC GACGGCGCGC CTGCGGCCGC TTAATTAAGG CCTTGAATCG
351 AGAATTTATA CTTAGATAAG TATGTACTTA CAGGTATATT TCTATGAGAT
401 ACTGATGTAT ACATGCATGA TAATATTTAA ACGGTTATTA GTGCCGATTG
451 TCTTGTGCGA TAATGACGTT CCTATCAAAG CAATACACTT ACCACCTATT
501 ACATGGGCCA AGAAAATATT TTCGAACTTG TTTAGAATAT TAGCACAGAG
551 TATATGATGA TATCCGTTAG ATTATGCATG ATTCATTCCT ACAACTTTTT
601 CGTAGCATAA GGATTAATTA CTTGGATGCC AATAAAAAAA AAAAACATCG
651 AGAAAATTTC AGCATGCTCA GAAACAATTG CAGTGTATCA AAGTAAAAAA
701 AAGATTTTCA CTACATGTTC CTTTTGAAGA AAGAAAATCA TGGAACATTA
751 GATTTACAAA AATTTAACCA CCGCTGATTA ACGATTAGAC CGTTAAGCGC
801 ACAACAGGTT ATTAGTACAG AGAAAGCATT CTGTGGTGTT GCCCCGGACT
851 TTCTTTTGCG ACATAGGTAA ATCGAATACC ATCATACTAT CTTTTCCAAT
901 GACTCCCTAA AGAAAGACTC TTCTTCGATG TTGTATACGT TGGAGCATAG
951 GGCAAGAATT GTGGCTTGAG ATCATCCTTT TGTTGTTTCC GGGTGTACAA
1001 TATGGACTTC CTCTTTTCTG GCAACCAAAC CCATACATCG GGATTCCTAT
1051 AATACCTTCG TTGGTCTCCC TAACATGTAG GTGGCGGAGG GGAGATATAC
1101 AATAGAACAG ATACCAGACA AGACATAATG GGCTAAACAA GACTACACCA
1151 ATTACACTGC CTCATTGATG GTGGTACATA ACGAACTAAT ACTGTAGCCC
1201 TAGACTTGAT AGCCATCATC ATATCGAAGT TTCACTACCC TTTTTCCATT
1251 TGCCATCTAT TGAAGTAATA ATAGGCGCAT GCAACTTCTT TTCTTTTTTT
1301 TTCTTTTCTC TCTCCCCCGT TGTTGTCTCA CCATATCCGC AATGACAAAA
1351 AAATGATGGA AGACACTAAA GGAAAAAATT AACGACAAAG ACAGCACCAA
1401 CAGATGTCGT TGTTCCAGAG CTGATGAGGG GTATCTCGAA GCACACGAAA
1451 CTTTTTCCTT CCTTCATTCA CGCACACTAC TCTCTAATGA GCAACGGTAT
1501 ACGGCCTTCC TTCCAGTTAC TTGAATTTGA AATAAAAAAA AGTTTGCTGT
1551 CTTGCTATCA AGTATAAATA GACCTGCAAT TATTAATCTT TTGTTTCCTC
1601 GTCATTGTTC TCGTTCCCTT TCTTCCTTGT TTCTTTTTCT GCACAATATT
1651 TCAAGCTATA CCAAGCATAC AATCAAGGAA TTCCGGATCC GCCACCATGC
1701 CTCAATCCTG GGAAGAACTG GCCGCTGATA AGCGCGCCCG CCTCGCAAAA
1751 ACCATCCCTG ATGAATGGAA AGTCCAGACG CTGCCTGCGG AAGACAGCGT
1801 TATTGATTTC CCAAAGAAAT CGGGGATCCT TTCAGAGGCC GAACTGAAGA
1851 TCACAGAGGC TTCCGCTGCG GATCTTGTGT CCAAGCTGGC GGCCGGAGAG
1901 TTGACCTCGG TGGAAGTTAC GCTAGCATTC TGTAAACGGG CAGCAATCGC
1951 CCAGCAGTTA ACAAACTGCG CCCACGAGTT CTTCCCTGAC GCCGCTCTCG
2001 CGCAGGCAAG GGAACTCGAT GAATACTACG CAAAGCACAA GAGACCCGTT
2051 GGTCCACTCC ATGGCCTCCC CATCTCTCTC AAAGACCAGC TTCGAGTCAA
2101 GGGCTACGAA ACATCAATGG GCTACATCTC ATGGCTAAAC AAGTACGACG
2151 AAGGGGACTC GGTTCTGACA ACCATGCTCC GCAAAGCCGG TGCCGTCTTC
2201 TACGTCAAGA CCTCTGTCCC GCAGACCCTG ATGGTCTGCG AGACAGTCAA
2251 CAACATCATC GGGCGCACCG TCAACCCACG CAACAAGAAC TGGTCGTGCG
2301 GCGGCAGTTC TGGTGGTGAG GGTGCGATCG TTGGGATTCG TGGTGGCGTC
2351 ATCGGTGTAG GAACGGATAT CGGTGGCTCG ATTCGAGTGC CGGCCGCGTT
2401 CAACTTCCTG TACGGTCTAA GGCCGAGTCA TGGGCGGCTG CCGTATGCAA
2451 AGATGGCGAA CAGCATGGAG GGTCAGGAGA CGGTGCACAG CGTTGTCGGG
2501 CCGATTACGC ACTCTGTTGA GGACCTCCGC CTCTTCACCA AATCCGTCCT
2551 CGGTCAGGAG CCATGGAAAT ACGACTCCAA GGTCATCCCC ATGCCCTGGC
2601 GCCAGTCCGA GTCGGACATT ATTGCCTCCA AGATCAAGAA CGGCGGGCTC
2651 AATATCGGCT ACTACAACTT CGACGGCAAT GTCCTTCCAC ACCCTCCTAT
2701 CCTGCGCGGC GTGGAAACTA CCGTCGCCGC ACTCGCCAAA GCCGGTCACA
2751 CCGTGACCCC GTGGACGCCA TACAAGCACG ATTTCGGCCA CGATCTCATC
2801 TCCCATATCT ACGCGGCTGA CGGCAGCGCC GACGTAATGC GCGATATCAG
2851 TGCATCCGGC GAGCCGGCGA TTCCAAATAT CAAAGACCTA CTGAACCCGA
2901 ACATCAAAGC TGTTAACATG AACGAGCTCT GGGACACGCA TCTCCAGAAG
2951 TGGAATTACC AGATGGAGTA CCTTGAGAAA TGGCGGGAGG CTGAAGAAAA
3001 GGCCGGGAAG GAACTGGACG CCATCATCGC GCCGATTACG CCTACCGCTG
3051 CGGTACGGCA TGACCAGTTC CGGTACTATG GGTATGCCTC TGTGATCAAC
3101 CTGCTGGATT TCACGAGCGT GGTTGTTCCG GTTACCTTTG CGGATAAGAA
3151 CATCGATAAG AAGAATGAGA GTTTCAAGGC GGTTAGTGAG CTTGATGCCC
3201 TCGTGCAGGA AGAGTATGAT CCGGAGGCGT ACCATGGGGC ACCGGTTGCA
3251 GTGCAGGTTA TCGGACGGAG ACTCAGTGAA GAGAGGACGT TGGCGATTGC
3301 AGAGGAAGTG GGGAAGTTGC TGGGAAATGT GGTGACTCCA TAGCCCGGGG
3351 GGGGCTCGAT CCCCTCGCGA GTTGGTTCAG CTGCTGCCTG AGGCTGGACG
3401 ACCTCGCGGA GTTCTACCGG CAGTGCAAAT CCGTCGGCAT CCAGGAAACC
3451 AGCAGCGGCT ATCCGCGCAT CCATGCCCCC GAACTGCAGG AGTGGGGAGG
3501 CACGATGGCC GCTTTGGTCG ATCTAGATTA CGTGGAAGAA AGGTAGTAAA
3551 AGTAGTAGTA TAAGTAGTAA AAAGAGGTAA AAAGAGAAAA CCGGCTACAT
3601 ACTAGAGAAG CACGTACACA AAAACTCATA GGCACTTCAT CATACGACAG
3651 TTTCTTGATG CATTATAATA GTGTATTAGA TATTTTCAGA AATATGCATA
3701 GAACCTCCTC TTGCCTTTAC TTTTTATACA TAGAACATTG GCAGATTTAC
3751 TTACACTACT TTGTTTCTAC GCCATTTCTT TTGTTTTCAA CACTTAGACA
3801 AGTTGTTGAG AACCGGACTA CTAAAAAGCA ATGTTCCCAC TGAAAATCAT
3851 GTACCTGCAG GATAATAACC CCCTAATTCT GCATCGATCC AGTATGTTTT
3901 TTTTTCTCTA CTCATTTTTA CCTGAAGATA GAGCTTCTAA AACAAAAAAA
3951 ATCAGTGATT ACATGCATAT TGTGTGTTCT AGTAACCAAA GGAAAGGAAC
4001 AGATAGATAA AATTCCGAGA CTGTCAAATT AGGTTTTTTT CTTTTTTTTT
4051 GGCGGGAGTC AGTGGGCCGA AATATGTTCT TGGCCTAGAA CTTAATCTGG
4101 TTTGATCATG CCAATACTTG CCTGAGTGCC CGACTTTTTG CCCACCCTCT
4151 TGCCTTCTGT CATCCTTCAA AACCCACCTG TTTTCCAGCC GTATCTTCGC
4201 TCGCATCTAC ACATACTGTG CCATATCTTG TGTGTAGCCG GACGTGACTA
4251 TGACCAAAAA CAAACAAGGA GAACTGTTCG CCGATTTGTA ACACTCCTGC
4301 ATCCATCCAA GTGGGTATGC GCTATGCAAT GTTAAGCTAG GTCAGGTCAG
4351 ACCAGGTCCA AGGACAGCAA CTTGACTGTA TGCAACCTTT ACCATCTTTG
4401 CACAGAACAT ACTTGTAGCT AGCTAGTTAC ACTTATGGAC CGAAAAGGCA
4451 CCCCACCATG TCTGTCCGGC TTTAGAGTAC GGCCGCAGAC CGCTGATTTG
4501 CCTTGCCAAG CAGTAGTCAC AATGCATCGC ATGAGCACAC GGGCACGGGC
4551 ACGGGCACAG GAACCATTGG CAAAAATACC AGATACACTA TACCGACGTA
4601 TATCAAGCCC AAGTTTAAAA TTCCTAAATT TCCGCGGCTA CTTTTCAATT
4651 CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG
4701 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
4751 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT
4801 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
4851 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
4901 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
4951 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
5001 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
5051 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
5101 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
5151 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
5201 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
5251 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
5301 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
5351 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
5401 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
5451 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
5501 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
5551 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
5601 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
5651 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
5701 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
5751 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
5801 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
5851 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
5901 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
5951 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
6001 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
6051 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
6101 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
6151 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
6201 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
6251 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
6301 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
6351 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
6401 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
6451 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
6501 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
6551 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
6601 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
6651 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
6701 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
6751 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
6801 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC
6851 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC
6901 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
6951 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT
7001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA
7051 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC
7101 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA
7151 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG
7201 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
7251 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC
7301 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
7351 CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG
7401 GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG
7451 CCAAGCTCCC GCGGGGATCG ACTCATAAAA TAGTAACCTT CTAATGCGTA
7501 TCTATTGACT ACCAACCATT AGTGTGGTTG CAGAAGGCGG AATTCTCCCT
7551 TCTTCGAATT CAGCTTGCTT TTTCATTTTT TATTTTCCAT TTTTCAGTTT
7601 TTGTTTGTGT CGAATTTAGC CAGTTGCTTC TCCAAGATGA AAAAAACCCC
7651 TGCGCAGTTT CTGTGCTGCA AGATCCTAAT CGACTTTTCC ACCCCCCACA
7701 AAAGTAAATG TTCTTTTGTT ACATTCGCGT GGGTAGCTAG CTCCCCGAAT
7751 CTTCAAAGGA CTTAGGGACT GCACTACATC AGAGTGTGTT CACCTGGTTT
7801 GCTGCCTGGT TTGAAAGAAA AGAGCAGGGA ACTCGCGGGT TCCCGGCGAA
7851 TAATCATGCG ATAGTCCTTT GGCCTTCCAA GTCGCATGTA GAGTAGACAA
7901 CAGACAGGGA GGGCAGGAAG GATCTTTCAC TGAGATCCTG TATCTTGTTG
7951 GGTAAGTCGG ATGAAAGGGG AATCGTATGA GATTGGAGAG GATGCGGAAG
8001 AGGTAACGCC TTTTGTTAAC TTGTTTAATT ATTATGGGGC AGGCGAGAGG
8051 GGGAGGAATG TATGTGTGTG AGGCGGGCGA GACGGAGCCA TCCAGGCCAG
8101 GTAGAAATAG AGAAAGCCGA ATGTTAGACA ATATGGCAGC GTAGTAGAGT
8151 AGGTAGGTAG GCAAGTACTG CTAGCAAAGA GGAGAAGGGT AAGCTCACTC
8201 TTCGCATTCC ACACCGTTAG TGTGTCAGTT TGAACAAAAA AACAATCATC
8251 ATACCAATTG ATGGACTGTG GACTGGCTTT TGGAACGGCT TTTCGGACTG
8301 CGATTATTCG TGAGGAATCA AGGTAGGAAT TTGGTCATAT TTACGGACAA
8351 CAGTGGGTGA TTCCCATATG GAGTAGGAAA ACGAGATCAT GGTATCCTCA
8401 GATATGTTGC GGAATTCTGT TCACCGCAAA GTTCAGGGTG CTCTGGTGGG
8451 TTTCGGTTGG TCTTTGCTTT GCTTCTCCCT TGTCTTGCAT GTTAATAATA
8501 GCCTAGCCTG TGAGCCGAAA CTTAGGGTAG GCTTAGTGTT GGAACGTACA
8551 TATGTATCAC GTTGACTTGG TTTAACCAGG CGACCTGGTA GCCAGCCATA
8601 CCCACACACG TTTTTTGTAT CTTCAGTATA GTTGTGAAAA GTGTAGCGGA
8651 AATTTGTGGT CCGAGCAACA GCGTCTTTTT CTAGTAGTGC GGTCGGTTAC
8701 TTGGTTGACA TTGGTATTTG GACTTTGTTG CTACACCATT CACTACTTGA
8751 AGTCGAGTGT GAAGGGTATG ATTTCTAGTG GTGAACACCT TTAGTTACGT
8801 AATGTTTTCA TTGCTGTTTT ACTTGAGATT TCGATTGAGA AAAAGGTATT
8851 TAATAGCTCG AATCAATGTG AGAACAGAGA GAAGATGTTC TTCCCTAACT
8901 CGAAAGGTAT ATGAGGCTTG TGTTTCTTAG GAGAATTATT ATTCTTTTGT
8951 TATGTTGCGC TTGTAGTTGG AAAAGGTGAA GAGACAAAAG CTGGAATTGT
9001 GAGCGGATAA CAAGCTCAAC ACTTGAAATT TAGGAAAGAG CAGAATTTGG
9051 CAAAAAAAAT AAAAAAAAAA TAAACACACA TACTCATCGA G
