View source for pGEM-2

==At a Glance ==

[[Image:PGEM2.jpg|thumb|450px|right|pGEM-2, Key: red = marker, magenta = promoters, stripes = MCS]]
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!align=left {{table heading style}} |Type
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Cloning
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!align=left {{table heading style}} |Origin of Replication
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|-
!align=left {{table heading style}} |Copy #
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|-
!align=left {{table heading style}} |Markers
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Ampicillin
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!align=left {{table heading style}} |Link to Sequence
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58197 PubMed]
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!align=left {{table heading style}} |Features
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SP6 promoter<br>   
T7 promoter<br>    
multiple cloning sites<br> 
lac operon<br> 
 
EcoR I, Sac  I, Ava  I, Sma  I, BamH I, Xba  I, Sal I, Acc  I, Hinc II, Pst  I, Hind III
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!align=left {{table heading style}} |Accession Number
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65301 X65301]

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==Notes==

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/products/pm/discontinued-vectors/#DV8 Promega]. Discontinued.

==References==
{{BacktoTop}}
{{RefHelp}}
<references />

==Sequence ==
        1 gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
       61 ccggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta atgaatcggc
      121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
      781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     2161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     2221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     2281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     2341 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     2401 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     2461 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     2521 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     2581 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
     2641 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
     2701 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
     2761 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
     2821 taatacataa ccttatgtat catacacata cgatttaggt gacactata




[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
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