pGEM-2

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At a Glance

pGEM-2, Key: red = marker, magenta = promoters, stripes = MCS

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Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

PubMed

Features

SP6 promoter
T7 promoter
multiple cloning sites
lac operon

EcoR I, Sac I, Ava I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Hind III

Accession Number

X65301

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Notes

History

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Sources

Promega. Discontinued.

References

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Sequence

       1 gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
      61 ccggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta atgaatcggc
     121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    1021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    1081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    1141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    1201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    1261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    1321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    1381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    1441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    1501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    1561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    1621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    1681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    1741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    1801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    1861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    1921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    1981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    2041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    2101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    2161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    2221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    2281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    2341 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
    2401 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
    2461 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
    2521 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
    2581 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
    2641 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
    2701 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
    2761 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
    2821 taatacataa ccttatgtat catacacata cgatttaggt gacactata