pGEM-2
At a Glance
<protect>
Type |
Cloning |
---|---|
Origin of Replication | |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
SP6 promoter EcoR I, Sac I, Ava I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Hind III |
Accession Number | |
edit table |
</protect>
Notes
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt 61 ccggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta atgaatcggc 121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 1801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 1861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 1921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 1981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 2101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 2161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 2221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 2281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 2341 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga 2401 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc 2461 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt 2521 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg 2581 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc 2641 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag 2701 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc 2761 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat 2821 taatacataa ccttatgtat catacacata cgatttaggt gacactata