View source for pFN6K (HQ)

==At a Glance==
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[[File:PFN6K (HQ).jpg|thumb|450px|pFN6K (HQ), Image made with [[PlasMapper]]]]
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!align=left {{table heading style}} |Type
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Cloning
|-
!align=left {{table heading style}} |Origin of Replication
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ColE1
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!align=left {{table heading style}} |Copy #
||

|-
!align=left {{table heading style}} |Markers
||
Kanamycin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.ncbi.nlm.nih.gov/nuccore/73697470? NCBI]
|-
!align=left {{table heading style}} |Features
||
T7 Promoter <br /> 
HQHQHQ N Terminus <br /> 
Sgf I site <br /> 
Pme I site <br />
cer site <br /> 
rrnB transcription terminator <br /> 
T7 terminator 
|-
!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=73697470 DQ132630]

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==Notes==
This vector is one of two vectors, designed for expressing N-terminal, HisGln (HQ) metal-binding peptide fusion proteins in bacteria and in vitro protein expression systems. It contains a HQ tag that is used to purify protein expression using the MagneHis, MagZ, HisLink 96 or HisLink Protein Purification Systems. This vector is  designed specifically for bacterial or in vitro protein putification through the T7 RNA polymerase promoter. Compared to pFN6A (HQ), this vector is kanamycin resistance. In addition, it functions in protein purification via a metal affinity resin.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/search-results/?q=pFN6K%20(HQ) Promega]

==References==
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<references />

==Sequence==
<pre>
    1  GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGC AATTTTAACT
   51  TTACTAAGGA GAATTCACCA TGAAACATCA ACATCAGCAC CAAGCGATCG
  101  CCATGGAATA AGTAAGGAAT CCACATGGCA CAGGTTATCA ACACGTTTGA
  151  CGGGGTTGCG GATTATCTTC AGACATATCA TAAGCTACCT GATAATTACA
  201  TTACAAAATC AGAAGCACAA GCCCTCGGCT GGGTGGCATC AAAAGGGAAC
  251  CTTGCAGACG TCGCTCCGGG GAAAAGCATC GGCGGAGACA TCTTCTCAAA
  301  CAGGGAAGGC AAACTCCCGG GCAAAAGCGG ACGAACATGG CGTGAAGCGG
  351  ATATTAACTA TACATCAGGC TTCAGAAATT CAGACCGGAT TCTTTACTCA
  401  AGCGACTGGC TGATTTACAA AACAACGGAC CATTATCAGA CCTTTACAAA
  451  AATCAGATAA TGTTTAAACG AATTCGGGCT CGGTACCCGG GGATCCTCTA
  501  GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC AAAGCCCGAA
  551  AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC
  601  CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC
  651  TATATCCGGT TCGCTTGCTG TCCATAAAAC CGCCCAGTCT AGCTATCGCC
  701  ATGTAAGCCC ACTGCAAGCT ACCTGCTTTC TCTTTGCGCT TGCGTTTTCC
  751  CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC AGCACCGTTT
  801  CTGCGGACTG GCTTTCTACG TGTTCCGCTT CCTTTAGCAG CCCTTGCGCC
  851  CTGAGTGCTT GCGGCAGCGT GAGCTTCAAA AGAATTGCCA GCTGGGGCGC
  901  CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT GGCTTTCTTG
  951  CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCTGATC AAGAGACAGG
 1001  ATGACGGTCG TTTCGCATGC TTGAACAAGA TGGATTGCAC GCAGGTTCTC
 1051  CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
 1101  ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
 1151  GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG
 1201  ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
 1251  GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
 1301  CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
 1351  AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
 1401  GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
 1451  CACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
 1501  ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGTATG
 1551  CCGGATGGTG AGGATCTCGT CGTGACTCAT GGCGATGCCT GCTTGCCGAA
 1601  TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
 1651  TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT
 1701  GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG
 1751  TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG
 1801  AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
 1851  CCAACCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
 1901  CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
 1951  AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
 2001  CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
 2051  CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
 2101  CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
 2151  CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
 2201  GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
 2251  GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
 2301  CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
 2351  TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
 2401  CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
 2451  GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
 2501  AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
 2551  CGCAGAAAAA AAGGATTTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
 2601  TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
 2651  TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG AAATACAGGA
 2701  ACGCACGCTG GATGGCCCTT CGCTGGGATG GTGAAACCAT GAAAAATGGC
 2751  AGCTTCAGTG GATTAAGTGG GGGTAATGTG GCCTGTACCC TCTGGTTGCA
 2801  TAGGTATTCA TACGGTTAAA ATTTATCAGG CGCGATTGCG GCAGTTTTTC
 2851  GGGTGGTTTG TTGCCATTTT TACCTGTCTG CTGCCGTGAT CGCGCTGAAC
 2901  GCGTTTTAGC GGTGCGTACA ATTAAGGGAT TATGGTAAAT CCACTTACTG
 2951  TCTGCCCTCG TAGCCATCGA GATAAACCGC AGTACTCCGG CCACGATGCG
 3001  TCCGGCGTAG AGGATCGAGA TCTTTTCAGC CTGATACAGA TTAAATCAGA
 3051  ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC AGTAGCGCGG
 3101  TGGTCCCACC TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC
 3151  GATGGTAGTG TGGGGTCTCC CCATGCGAGA GTAGGGAACT GCCAGGCATC
 3201  AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT
 3251  TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA
 3301  TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC
 3351  CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC
 3401  CTTTTTGCGT TTCTACAAAC TCTTTTGTTT ATTTTTCTAA ATACATTCAA
 3451  ATATGTATCC GCTCAT
</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Promega Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:T7 Promoter]]