pFN6K (HQ)
At a Glance
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pFN6K (HQ), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
This vector is one of two vectors, designed for expressing N-terminal, HisGln (HQ) metal-binding peptide fusion proteins in bacteria and in vitro protein expression systems. It contains a HQ tag that is used to purify protein expression using the MagneHis, MagZ, HisLink 96 or HisLink Protein Purification Systems. This vector is designed specifically for bacterial or in vitro protein putification through the T7 RNA polymerase promoter. Compared to pFN6A (HQ), this vector is kanamycin resistance. In addition, it functions in protein purification via a metal affinity resin.
History
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Sources
References
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Sequence
1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGC AATTTTAACT 51 TTACTAAGGA GAATTCACCA TGAAACATCA ACATCAGCAC CAAGCGATCG 101 CCATGGAATA AGTAAGGAAT CCACATGGCA CAGGTTATCA ACACGTTTGA 151 CGGGGTTGCG GATTATCTTC AGACATATCA TAAGCTACCT GATAATTACA 201 TTACAAAATC AGAAGCACAA GCCCTCGGCT GGGTGGCATC AAAAGGGAAC 251 CTTGCAGACG TCGCTCCGGG GAAAAGCATC GGCGGAGACA TCTTCTCAAA 301 CAGGGAAGGC AAACTCCCGG GCAAAAGCGG ACGAACATGG CGTGAAGCGG 351 ATATTAACTA TACATCAGGC TTCAGAAATT CAGACCGGAT TCTTTACTCA 401 AGCGACTGGC TGATTTACAA AACAACGGAC CATTATCAGA CCTTTACAAA 451 AATCAGATAA TGTTTAAACG AATTCGGGCT CGGTACCCGG GGATCCTCTA 501 GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC AAAGCCCGAA 551 AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC 601 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC 651 TATATCCGGT TCGCTTGCTG TCCATAAAAC CGCCCAGTCT AGCTATCGCC 701 ATGTAAGCCC ACTGCAAGCT ACCTGCTTTC TCTTTGCGCT TGCGTTTTCC 751 CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC AGCACCGTTT 801 CTGCGGACTG GCTTTCTACG TGTTCCGCTT CCTTTAGCAG CCCTTGCGCC 851 CTGAGTGCTT GCGGCAGCGT GAGCTTCAAA AGAATTGCCA GCTGGGGCGC 901 CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT GGCTTTCTTG 951 CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCTGATC AAGAGACAGG 1001 ATGACGGTCG TTTCGCATGC TTGAACAAGA TGGATTGCAC GCAGGTTCTC 1051 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 1101 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC 1151 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG 1201 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 1251 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG 1301 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 1351 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 1401 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG 1451 CACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC 1501 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGTATG 1551 CCGGATGGTG AGGATCTCGT CGTGACTCAT GGCGATGCCT GCTTGCCGAA 1601 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 1651 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 1701 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG 1751 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG 1801 AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 1851 CCAACCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 1901 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 1951 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 2001 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 2051 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 2101 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 2151 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 2201 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 2251 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 2301 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 2351 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 2401 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 2451 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 2501 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 2551 CGCAGAAAAA AAGGATTTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 2601 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 2651 TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG AAATACAGGA 2701 ACGCACGCTG GATGGCCCTT CGCTGGGATG GTGAAACCAT GAAAAATGGC 2751 AGCTTCAGTG GATTAAGTGG GGGTAATGTG GCCTGTACCC TCTGGTTGCA 2801 TAGGTATTCA TACGGTTAAA ATTTATCAGG CGCGATTGCG GCAGTTTTTC 2851 GGGTGGTTTG TTGCCATTTT TACCTGTCTG CTGCCGTGAT CGCGCTGAAC 2901 GCGTTTTAGC GGTGCGTACA ATTAAGGGAT TATGGTAAAT CCACTTACTG 2951 TCTGCCCTCG TAGCCATCGA GATAAACCGC AGTACTCCGG CCACGATGCG 3001 TCCGGCGTAG AGGATCGAGA TCTTTTCAGC CTGATACAGA TTAAATCAGA 3051 ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC AGTAGCGCGG 3101 TGGTCCCACC TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC 3151 GATGGTAGTG TGGGGTCTCC CCATGCGAGA GTAGGGAACT GCCAGGCATC 3201 AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT 3251 TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA 3301 TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC 3351 CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC 3401 CTTTTTGCGT TTCTACAAAC TCTTTTGTTT ATTTTTCTAA ATACATTCAA 3451 ATATGTATCC GCTCAT
