View source for pF5A CMV-neo

==At a Glance==

__NOTOC__

[[File:PF5A CMV-neo.jpg|thumb|450px|pF5A CMV-neo, Image made with [[PlasMapper]]]]

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!align=left {{table heading style}} |Type
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Cloning
|-
!align=left {{table heading style}} |Origin of Replication
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ColE1
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!align=left {{table heading style}} |Copy #
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|-
!align=left {{table heading style}} |Markers
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Ampicillin, Neomycin
|-
!align=left {{table heading style}} |Link to Sequence
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[http://www.ncbi.nlm.nih.gov/nuccore/109900614?report=fasta NCBI]
|-
!align=left {{table heading style}} |Features
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CMV enhancer/promoter <br />
T7 Promoter <br /> 
Sgf I site <br /> 
Barnase coding region <br /> 
Pme I site <br /> 
SV40 enhancer and early promoter <br /> 
neomycin phosphotransferase coding region <br />  
cer site 
|-
!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=109900614 DQ487155]

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==Notes==
This plasmid is is used for constitutive expression in mammalian cells with neomycin selection. In addition, it is also used for expression of native protein with selection for stable transfectants
pF5A CMV-neo and pF5K CMv-neo are in the same group with differing selection sites.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/flexi-vectors/untagged-flexi-mammalian-expression-vectors/ Promega]

==References==
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<references />

==Sequence==
<pre>
    1  TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA
   51  ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC
  101  ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT
  151  GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
  201  ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA
  251  CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
  301  AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC
  351  GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG
  401  CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
  451  GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
  501  TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT
  551  GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
  601  CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC
  651  GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG
  701  GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG
  751  CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT
  801  CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT
  851  GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA
  901  ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA
  951  TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC
 1001  CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG
 1051  GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT
 1101  CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC
 1151  CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA
 1201  TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA
 1251  CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT
 1301  GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG
 1351  ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA
 1401  GACCTTTACA AAAATCAGAT AATGTTTAAA CGAATTCGGG CTCGGTACCC
 1451  GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA
 1501  ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA
 1551  CTAGCATAAC CCCTTGGGGC GGCCGCTTCG AGCAGACATG ATAAGATACA
 1601  TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT
 1651  ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
 1701  CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
 1751  AGGGGGAGAT GTGGGAGGTT TTTTTAAGCA AGTAAAACCT CTACAAATGT
 1801  GGTAAAATCG AATTTTAACA AAATATTAAC GCTTACAATT TCCTGATGCG
 1851  GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGCGGAT
 1901  CTGCGCAGCA CCATGGCCTG AAATAACCTC TGAAAGAGGA ACTTGGTTAG
 1951  GTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG
 2001  TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
 2051  TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
 2101  GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG
 2151  CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
 2201  TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG
 2251  CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC
 2301  CTAGGCTTTT GCAAAAAGCT TGATTCTTCT GACACAACAG TCTCGAACTT
 2351  AAGGCTAGAG CCACCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
 2401  GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
 2451  TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
 2501  GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA
 2551  CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
 2601  CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
 2651  GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
 2701  AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
 2751  CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
 2801  ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
 2851  TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
 2901  CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
 2951  ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT
 3001  GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
 3051  CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT
 3101  ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA
 3151  GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC
 3201  CAACCTGCCA TCACGATGGC CGCAATAAAA TATCTTTATT TTCATTACAT
 3251  CTGTGTGTTG GTTTTTTGTG TGAATCGATA GCGATAAGGA TCCTCTTTGC
 3301  GCTTGCGTTT TCCCTTGTCC AGATAGCCCA GTAGCTGACA TTCATCCGGG
 3351  GTCAGCACCG TTTCTGCGGA CTGGCTTTCT ACGTAATGGT TTCTTAGACG
 3401  TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
 3451  TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
 3501  AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
 3551  CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
 3601  TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
 3651  CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG
 3701  AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TCAAAGTTCT
 3751  GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
 3801  GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
 3851  ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
 3901  TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACTA
 3951  TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT
 4001  GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
 4051  CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
 4101  AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
 4151  GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
 4201  TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT
 4251  CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC
 4301  GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
 4351  ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAATTCGAAA
 4401  TGACCGACCA AGCGACGCCC AACCGGTATC AGCTCACTCA AAGGCGGTAA
 4451  TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
 4501  AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
 4551  TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
 4601  GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
 4651  CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
 4701  ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
 4751  CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
 4801  TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
 4851  CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
 4901  CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
 4951  GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
 5001  TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
 5051  GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
 5101  TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATTTCAAG AAGATCCTTT
 5151  GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
 5201  GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
 5251  TAGTCCGGAA ATACAGGAAC GCACGCTGGA TGGCCCTTCG CTGGGATGGT
 5301  GAAACCATGA AAAATGGCAG CTTCAGTGGA TTAAGTGGGG GTAATGTGGC
 5351  CTGTACCCTC TGGTTGCATA GGTATTCATA CGGTTAAAAT TTATCAGGCG
 5401  CGATTGCGGC AGTTTTTCGG GTGGTTTGTT GCCATTTTTA CCTGTCTGCT
 5451  GCCGTGATCG CGCTGAACGC GTTTTAGCGG TGCGTACAAT TAAGGGATTA
 5501  TGGTAAATCC ACTTACTGTC TGCCCTCGTA GCCATCGAGA TAAACCGCAG
 5551  TACTCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC T
</pre>


[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Neomycin Marker Plasmids]][[Category:T7 Promoter]][[Category:CMV promoter]]