pF5A CMV-neo
At a Glance
pF5A CMV-neo, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Ampicillin, Neomycin |
| Link to Sequence | |
| Features |
CMV enhancer/promoter |
| Accession Number | |
| edit table |
</protect>
Notes
This plasmid is is used for constitutive expression in mammalian cells with neomycin selection. In addition, it is also used for expression of native protein with selection for stable transfectants pF5A CMV-neo and pF5K CMv-neo are in the same group with differing selection sites.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAA CGAATTCGGG CTCGGTACCC 1451 GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA 1501 ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA 1551 CTAGCATAAC CCCTTGGGGC GGCCGCTTCG AGCAGACATG ATAAGATACA 1601 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT 1651 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 1701 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1751 AGGGGGAGAT GTGGGAGGTT TTTTTAAGCA AGTAAAACCT CTACAAATGT 1801 GGTAAAATCG AATTTTAACA AAATATTAAC GCTTACAATT TCCTGATGCG 1851 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGCGGAT 1901 CTGCGCAGCA CCATGGCCTG AAATAACCTC TGAAAGAGGA ACTTGGTTAG 1951 GTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG 2001 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 2051 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 2101 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 2151 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 2201 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 2251 CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 2301 CTAGGCTTTT GCAAAAAGCT TGATTCTTCT GACACAACAG TCTCGAACTT 2351 AAGGCTAGAG CCACCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 2401 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 2451 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 2501 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA 2551 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 2601 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 2651 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 2701 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 2751 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 2801 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA 2851 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 2901 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 2951 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 3001 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 3051 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 3101 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA 3151 GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC 3201 CAACCTGCCA TCACGATGGC CGCAATAAAA TATCTTTATT TTCATTACAT 3251 CTGTGTGTTG GTTTTTTGTG TGAATCGATA GCGATAAGGA TCCTCTTTGC 3301 GCTTGCGTTT TCCCTTGTCC AGATAGCCCA GTAGCTGACA TTCATCCGGG 3351 GTCAGCACCG TTTCTGCGGA CTGGCTTTCT ACGTAATGGT TTCTTAGACG 3401 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 3451 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 3501 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 3551 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 3601 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 3651 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 3701 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TCAAAGTTCT 3751 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 3801 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 3851 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 3901 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACTA 3951 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 4001 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 4051 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 4101 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 4151 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 4201 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 4251 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 4301 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 4351 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAATTCGAAA 4401 TGACCGACCA AGCGACGCCC AACCGGTATC AGCTCACTCA AAGGCGGTAA 4451 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 4501 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 4551 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 4601 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 4651 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 4701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 4751 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 4801 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 4851 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 4901 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 4951 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 5001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 5051 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 5101 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATTTCAAG AAGATCCTTT 5151 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 5201 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 5251 TAGTCCGGAA ATACAGGAAC GCACGCTGGA TGGCCCTTCG CTGGGATGGT 5301 GAAACCATGA AAAATGGCAG CTTCAGTGGA TTAAGTGGGG GTAATGTGGC 5351 CTGTACCCTC TGGTTGCATA GGTATTCATA CGGTTAAAAT TTATCAGGCG 5401 CGATTGCGGC AGTTTTTCGG GTGGTTTGTT GCCATTTTTA CCTGTCTGCT 5451 GCCGTGATCG CGCTGAACGC GTTTTAGCGG TGCGTACAAT TAAGGGATTA 5501 TGGTAAATCC ACTTACTGTC TGCCCTCGTA GCCATCGAGA TAAACCGCAG 5551 TACTCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC T
