View source for pET-9a

==At a Glance==
[[File:PET-9a.jpg|thumb|450px|pET-9a Image made with [[PlasMapper]]]]
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!align=left {{table heading style}} |Type
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Cloning/Expression
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!align=left {{table heading style}} |Origin of Replication
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pBR322
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!align=left {{table heading style}} |Copy #
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~40
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!align=left {{table heading style}} |Markers
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Kanamycin
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!align=left {{table heading style}} |Link to Sequence
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[http://www.emdchemicals.com/life-science-research/vector-table-pet-9a-vector-sequence/c_jtmb.s1OzYcAAAEh.QALddSQ?WFSimpleSearch_NameOrID=pET-9a+sequence&BackButtonText=search+results Novagen]
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!align=left {{table heading style}} |Features
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T7 Promoter <br />
T7 Transcription start <br />
T7•Tag <br />
MCS <br />
T7 Terminator <br />
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!align=left {{table heading style}} |Accession Number
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==Notes==
pET-9a<ref name='PMID:15169771'/><ref name='PMID:11283016'/><ref name='PMID:8535158'/> is a 4341bp vector for cloning and expression.  pET vectors<ref name='PMID:15657041'/> pET-9a-d(+) possess an N-terminal T7•Tag and a MCS that includes a BamHI cloning site.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.emdbiosciences.com/product/69431 Novagen]<br>
[http://www.promega.com/vectors/bacterial_express_vectors.htm Promega]

==References==
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{{RefHelp}}
<references />
<ref name='PMID:15169771'/>
<ref name='PMID:11283016'/>
<ref name='PMID:8535158'/>
<ref name='PMID:15657041'/>
==Sequence==
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
      481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc 
      541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac 
      601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct 
      661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 
      721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 
      781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 
      841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 
      901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag 
      961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 
     1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 
     1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 
     1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 
     1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 
     1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 
     1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 
     1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg 
     1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 
     1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag 
     1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg 
     1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc 
     1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat 
     1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa 
     1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg 
     1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc 
     1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 
     1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc 
     2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc 
     2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat 
     2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa 
     2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga 
     2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 
     2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
     2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
     2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 
     2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc 
     2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 
     2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca 
     3481 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 
     3541 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat 
     3601 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag 
     3661 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca 
     3721 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat 
     3781 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca 
     3841 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg 
     3901 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta 
     3961 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt 
     4021 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg 
     4081 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt 
     4141 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac 
     4201 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg 
     4261 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg 
     4321 ctcgatgagt ttttctaaga a 





[[Category:Plasmids]][[Category:Cloning]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Promega Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:T7 Promoter]][[Category:T7 Tag]][[Category:MCS]]
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