pET-9a
At a Glance
pET-9a Image made with PlasMapper
<protect>
| Type |
Cloning/Expression |
|---|---|
| Origin of Replication |
pBR322 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
pET-9a[1][2][3] is a 4341bp vector for cloning and expression. pET vectors[4] pET-9a-d(+) possess an N-terminal T7•Tag and a MCS that includes a BamHI cloning site.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Shen, R et al. (2004) Escherichia coli nucleoside diphosphate kinase interactions with T4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. J. Biol. Chem. 279 32225-32 PubMed
- ↑ Dai, KS & Liew, CC (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J. Biol. Chem. 276 23992-9 PubMed
- ↑ Hoffman, BJ et al. (1995) Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21 (DE3): process control yielding high levels of metal-incorporated, soluble protein. Protein Expr. Purif. 6 646-54 PubMed
- ↑ Hamburger, AE et al. (2005) Steric accessibility of the HIV-1 gp41 N-trimer region. J. Biol. Chem. 280 12567-72 PubMed
Sequence
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc
541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac
601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag
961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg
1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca
3481 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
3541 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
3601 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
3661 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
3721 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
3781 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
3841 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
3901 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
3961 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
4021 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
4081 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
4141 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
4201 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
4261 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
4321 ctcgatgagt ttttctaaga a
- ↑ Shen, R et al. (2004) Escherichia coli nucleoside diphosphate kinase interactions with T4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. J. Biol. Chem. 279 32225-32 PubMed
- ↑ Dai, KS & Liew, CC (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J. Biol. Chem. 276 23992-9 PubMed
- ↑ Hoffman, BJ et al. (1995) Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21 (DE3): process control yielding high levels of metal-incorporated, soluble protein. Protein Expr. Purif. 6 646-54 PubMed
- ↑ Hamburger, AE et al. (2005) Steric accessibility of the HIV-1 gp41 N-trimer region. J. Biol. Chem. 280 12567-72 PubMed
