View source for pET-3d

==At a Glance==
[[Image:pET-3d.JPG|thumb|right|450px|pET-3d, Image provided by [http://www.genomics.agilent.com/files/Vectors/Maps/pdf/pet-3d.pdf Agilent-Genomics]]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O46eace0d048aa-->
<!--
******************************************************************************************
* 
*   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. ** 
* 
****************************************************************************************** -->
{| {{Prettytable}} width='30%' align=left  id="O46eace0d048aa"  class=" tableEdit PlasmidTable"  

|-
!align=left {{table heading style}} |Type
||
Cloning/Expression
|-
!align=left {{table heading style}} |Origin of Replication
||
pBR322
|-
!align=left {{table heading style}} |Copy #
||

|-
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.genomics.agilent.com/files/Vectors/Sequences/pet-3d_s.txt Agilent Genomics]
|-
!align=left {{table heading style}} |Features
||
T7 Promoter <br />
RBS <br />
NdeI cloning site <br />
T7 gene 10 leader <br />
BamHI cloning site <br />
T7 Terminator <br />

|-
!align=left {{table heading style}} |Accession Number
||


|- class="tableEdit_footer" 
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O46eace0d048aa&page=112506&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O46eace0d048aa--></protect>
<BR>
<Br clear='all'>

==Notes==
Same features & purpose of pET-3a and b and c.  Cloning & expression of recombinant proteins without any additional tags.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.stratagene.com/lit/vector.aspx Agilent Genomics]<br />
[http://www.emdbiosciences.com/product/69421 Novagen]

==References==
{{BacktoTop}}
{{RefHelp}}
<references />

==Sequence==
THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.
    1  TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT  
   51  TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTAGCAT GACTGGTGGA  
  101  CAGCAAATGG GTCGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG  
  151  TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC  
  201  TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT  
  251  ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC  
  301  AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG  
  351  CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC  
  401  AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA  
  451  GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG  
  501  TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT  
  551  GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT  
  601  ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC  
  651  GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA  
  701  GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT  
  751  TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC  
  801  TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA  
  851  TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT  
  901  TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG  
  951  GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT  
 1001  TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG  
 1051  TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA  
 1101  CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC  
 1151  AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA  
 1201  GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA  
 1251  ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA  
 1301  TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC  
 1351  CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG  
 1401  CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT  
 1451  AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG  
 1501  CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT  
 1551  GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG  
 1601  TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA  
 1651  ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG  
 1701  TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT  
 1751  TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA  
 1801  AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA  
 1851  AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG  
 1901  CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC  
 1951  AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG  
 2001  ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA  
 2051  GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG  
 2101  TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA  
 2151  CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG  
 2201  CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC  
 2251  AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC  
 2301  AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT  
 2351  TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC  
 2401  TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA  
 2451  TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG  
 2501  GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA  
 2551  ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG  
 2601  ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG  
 2651  GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG  
 2701  CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC  
 2751  TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA  
 2801  CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC  
 2851  AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC  
 2901  GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT  
 2951  CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG  
 3001  AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT  
 3051  AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT  
 3101  TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG  
 3151  ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG  
 3201  TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG  
 3251  GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC  
 3301  CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA  
 3351  CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG  
 3401  TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC  
 3451  TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC  
 3501  TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG  
 3551  ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC  
 3601  GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG  
 3651  CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT  
 3701  GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC  
 3751  GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC  
 3801  AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG  
 3851  CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA  
 3901  GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC  
 3951  GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC  
 4001  CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG  
 4051  CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG  
 4101  GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC  
 4151  AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA  
 4201  AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG  
 4251  AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA  
 4301  GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT  
 4351  TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG  
 4401  AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG  
 4451  TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA  
 4501  TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA  
 4551  TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG  
 4601  TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT


[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Stratagene Plasmids]][[Category:Novagen Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]][[Category:Agilent Plasmids]]
__NOTOC__