View source for pET-3b

==At a Glance==
[[Image:pET-3b.JPG|thumb|right|450px|pET-3b, Image provided by [http://www.genomics.agilent.com/files/Vectors/Maps/pdf/pet-3b.pdf Agilent-Genomics]]]
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!align=left {{table heading style}} |Type
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Cloning/Expression
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!align=left {{table heading style}} |Origin of Replication
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pBR322
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!align=left {{table heading style}} |Copy #
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|-
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
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[http://www.genomics.agilent.com/files/Vectors/Sequences/pet-3b_s.txt Agilent-Genomics]
|-
!align=left {{table heading style}} |Features
||
T7 Promoter <br />
RBS <br />
NdeI cloning site <br />
T7 gene 10 leader <br />
BamHI cloning site <br />
T7 Terminator <br />

|-
!align=left {{table heading style}} |Accession Number
||


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==Notes==
Same features & purpose of pET-3a.  Cloning & expression of recombinant proteins without any additional tags.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.genomics.agilent.com/files/Vectors/Maps/pdf/pet-3b.pdf Stratagene]<br>
[http://www.emdbiosciences.com/product/69419 Novagen]

==References==
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<references />

==Sequence==
THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.
    1  TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT  
   51  TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGGCTAGCA TGACTGGTGG  
  101  ACAGCAAATG GGTCGGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG  
  151  AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC  
  201  TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG  
  251  ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT  
  301  CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG  
  351  TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA  
  401  GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC  
  451  AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG  
  501  GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG  
  551  GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC  
  601  TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC  
  651  TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT  
  701  TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG  
  751  TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC  
  801  CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA  
  851  CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT  
  901  TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT  
  951  TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC  
 1001  CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA  
 1051  AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC  
 1101  AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA  
 1151  CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG  
 1201  CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA  
 1251  CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG  
 1301  GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT  
 1351  ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT  
 1401  TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA  
 1451  TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC  
 1501  GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC  
 1551  GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC  
 1601  CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG  
 1651  AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC  
 1701  TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT  
 1751  TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC  
 1801  CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG  
 1851  AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC  
 1901  TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA  
 1951  TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC  
 2001  AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC  
 2051  AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC  
 2101  AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA  
 2151  GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG  
 2201  TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT  
 2251  ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG  
 2301  ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG  
 2351  CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA  
 2401  CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC  
 2451  TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC  
 2501  TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA  
 2551  TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA  
 2601  CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG  
 2651  CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG  
 2701  TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC  
 2751  ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC  
 2801  AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT  
 2851  ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA  
 2901  CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG  
 2951  GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT  
 3001  TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG  
 3051  TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG  
 3101  ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA  
 3151  CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG  
 3201  GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA  
 3251  GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA  
 3301  GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT  
 3351  GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA  
 3401  TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC  
 3451  GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG  
 3501  CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA  
 3551  GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG  
 3601  ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC  
 3651  CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG  
 3701  GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG  
 3751  ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG  
 3801  CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC  
 3851  AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG  
 3901  AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA  
 3951  ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG  
 4001  GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC  
 4051  GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG  
 4101  CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC  
 4151  GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC  
 4201  GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA  
 4251  AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG  
 4301  AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC  
 4351  CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT  
 4401  TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC  
 4451  AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT  
 4501  CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA  
 4551  TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG  
 4601  CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT





[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Stratagene Plasmids]][[Category:Novagen Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]][[Category:Agilent Plasmids]]
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