pET-3b

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At a Glance

pET-3b, Image provided by Agilent-Genomics

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Type

Cloning/Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
RBS
NdeI cloning site
T7 gene 10 leader
BamHI cloning site
T7 Terminator

Accession Number


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Notes

Same features & purpose of pET-3a. Cloning & expression of recombinant proteins without any additional tags.

History

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Sources

Stratagene
Novagen

References

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Sequence

THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.

   1  TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT  
  51  TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGGCTAGCA TGACTGGTGG  
 101  ACAGCAAATG GGTCGGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG  
 151  AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC  
 201  TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG  
 251  ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT  
 301  CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG  
 351  TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA  
 401  GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC  
 451  AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG  
 501  GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG  
 551  GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC  
 601  TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC  
 651  TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT  
 701  TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG  
 751  TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC  
 801  CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA  
 851  CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT  
 901  TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT  
 951  TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC  
1001  CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA  
1051  AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC  
1101  AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA  
1151  CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG  
1201  CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA  
1251  CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG  
1301  GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT  
1351  ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT  
1401  TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA  
1451  TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC  
1501  GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC  
1551  GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC  
1601  CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG  
1651  AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC  
1701  TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT  
1751  TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC  
1801  CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG  
1851  AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC  
1901  TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA  
1951  TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC  
2001  AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC  
2051  AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC  
2101  AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA  
2151  GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG  
2201  TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT  
2251  ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG  
2301  ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG  
2351  CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA  
2401  CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC  
2451  TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC  
2501  TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA  
2551  TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA  
2601  CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG  
2651  CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG  
2701  TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC  
2751  ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC  
2801  AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT  
2851  ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA  
2901  CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG  
2951  GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT  
3001  TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG  
3051  TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG  
3101  ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA  
3151  CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG  
3201  GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA  
3251  GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA  
3301  GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT  
3351  GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA  
3401  TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC  
3451  GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG  
3501  CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA  
3551  GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG  
3601  ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC  
3651  CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG  
3701  GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG  
3751  ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG  
3801  CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC  
3851  AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG  
3901  AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA  
3951  ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG  
4001  GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC  
4051  GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG  
4101  CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC  
4151  GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC  
4201  GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA  
4251  AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG  
4301  AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC  
4351  CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT  
4401  TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC  
4451  AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT  
4501  CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA  
4551  TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG  
4601  CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT