View source for pGPS4

==At a Glance ==
[[Image:PGPS4.jpg|thumb|400px|right|pGPS4, Image provided by [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pGPS4and5_map.pdf NEB]]]<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.M46d87e6758145-->
<!--
******************************************************************************************
*
*   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->
{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Donor
|-
!{{table heading style}} |Origin of Replication
|
R6K
|-
!{{table heading style}} |Copy #
|
High
|-
!{{table heading style}} |Markers
|
Tetracycline, Chloramphenicol
|-
!{{table heading style}} |Link to Sequence
|
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pgps4.txt NEB]
|-
!{{table heading style}} |Features
|
Tn7R<br>
Tn7L<br>
Transprimer-4<ref name='PMID:8556868'/>
|-
!{{table heading style}} |Accession Number
|

|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M46d87e6758145&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.M46d87e6758145--></protect>
<BR>
<Br clear='all'>

==Notes==
Will not replicate in regular lab strains of E. coli because it depends on a replication initiation protein of plasmid origin, not usually present.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
pGPS4 is utilized in the GPS™-LS Linker Scanning System as the Transprimer-4 donor.

==Sources ==
[http://www.neb.com/nebecomm/products/productN7140.asp New England Biolabs]

==Sequence ==
  1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 
  51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 
 101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 
 151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 
 201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 
 251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 
 301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 
 351  GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT 
 401  AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA 
 451  CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC 
 501  ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA 
 551  GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG 
 601  TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA 
 651  CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA 
 701  AAGAGGTAAT GAAATGGCAT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT 
 751  ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG AGATGGCGGA 
 801  CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC 
 851  GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG 
 901  TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA 
 951  CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC 
 1001  CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA 
 1051  GCGGTCCAAT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA 
 1101  AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA 
 1151  CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG 
 1201  CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG 
 1251  GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC 
 1301  GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG 
 1351  CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG 
 1401  AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA 
 1451  GAAGACAGTC ATAAGTGCGG CGACAGTAGT CATGCCCCGC GCCCACCGGA 
 1501  AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC 
 1551  TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT 
 1601  GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA 
 1651  GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC 
 1701  ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 
 1751  ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC 
 1801  GTCCGGCGTA GAGGATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG 
 1851  TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA 
 1901  GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG 
 1951  CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG 
 2001  ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC 
 2051  TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG 
 2101  ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA 
 2151  CCGCATTAAA GCTTATCGAT GATAAGCGCT GGCATTGACC CTGAGTGATT 
 2201  TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG 
 2251  TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC 
 2301  TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC 
 2351  ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG 
 2401  CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG 
 2451  ACGCGGATGA ACAGGCAGAG CTCTTACTGT CATGCCATCC GTATGTTTAA 
 2501  ACACAATAAA GTCTTAAACT GAACAAAATA GATCTAAACT ATGACAATAA 
 2551  AGTCTTAAAC TAGACAGAAT AGTTGTAAAC TGAAATCAGT CCAGTTATGC 
 2601  TGTGAAAAAG CATACTGGAC TTTTGTTATG GCTAAAGCAA ACTCTTCATT 
 2651  TTCTGAAGTG CAAATTGCCC GTCGTATTAA AGAGGGGCGT GGGGTCGACG 
 2701  CGGCCGCTAA CTATAACGGT CCTAAGGTAG CGAGTTTGGC GCGCCAAACG 
 2751  ATATCGGATC CAGGCGTTTA AGGGCACCAA TAACTGCCTT AAAAAAATTA 
 2801  CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC 
 2851  TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 
 2901  GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC 
 2951  GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA 
 3001  AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
 3051  TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA 
 3101  TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG 
 3151  AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 
 3201  TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ATTCCGGATG 
 3251  AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT 
 3301  GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
 3351  GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC 
 3401  TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT 
 3451  TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT 
 3501  ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC 
 3551  GTGCCGATCA ACGTCTCATT TTCGCCAAAA GTTGGCCCAG GGCTTCCCGG 
 3601  TATCAACAGG GACACCAGGA TTTATTTATT CTGCGAAGTG ATCTTCCGTC 
 3651  ACAGGTATGG ATCCGATATC ATTTAAATCT AGGGATAACA GGGTAATACT 
 3701  AGTGTCGACC AACCAGATAA GTGAAATCTA GTTCCAAACT ATTTTGTCAT 
 3751  TTTTAATTTT CGTATTAGCT TACGACGCTA CACCCAGTTC CCATCTATTT 
 3801  TGTCACTCTT CCCTAAATAA TCCTTAAAAA CTCCATTTCC ACCCCTCCCA 
 3851  GTTCCCAACT TTATTGTGTT TAAACACCGT AAGATGCTTT TCAGTGACT
==References==
<ref name='PMID: 8556868'/>
<ref name='PMID: 9055068'/>
<ref name='PMID: 10666445'/>
<ref name='PMID: 8125283'/>
<ref name='PMID: 728998'/>
<references/>




__NOTOC__


[[Category:Plasmids]][[Category:NEB Plasmids]][[Category:Chloramphenicol Marker Plasmids]][[Category:Tetracycline Marker Plasmids]][[Category:Tn7R]][[Category:Tn7L]][[Category:Transprimer-4]]