pGPS4
At a Glance
pGPS4, Image provided by NEB
<protect>
| Type |
Donor |
|---|---|
| Origin of Replication |
R6K |
| Copy # |
High |
| Markers |
Tetracycline, Chloramphenicol |
| Link to Sequence | |
| Features |
Tn7R |
| Accession Number |
|
| edit table |
</protect>
Notes
Will not replicate in regular lab strains of E. coli because it depends on a replication initiation protein of plasmid origin, not usually present.
History
You can help EcoliWiki by writing about the history of this plasmid. pGPS4 is utilized in the GPS™-LS Linker Scanning System as the Transprimer-4 donor.
Sources
Sequence
1 GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 51 TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 101 CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 151 GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 201 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 251 ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 301 CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 351 GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT 401 AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA 451 CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC 501 ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA 551 GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG 601 TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA 651 CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA 701 AAGAGGTAAT GAAATGGCAT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT 751 ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG AGATGGCGGA 801 CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC 851 GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG 901 TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA 951 CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC 1001 CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA 1051 GCGGTCCAAT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA 1101 AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA 1151 CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG 1201 CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG 1251 GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC 1301 GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG 1351 CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG 1401 AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA 1451 GAAGACAGTC ATAAGTGCGG CGACAGTAGT CATGCCCCGC GCCCACCGGA 1501 AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC 1551 TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT 1601 GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA 1651 GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC 1701 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 1751 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC 1801 GTCCGGCGTA GAGGATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG 1851 TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA 1901 GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG 1951 CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG 2001 ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC 2051 TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG 2101 ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA 2151 CCGCATTAAA GCTTATCGAT GATAAGCGCT GGCATTGACC CTGAGTGATT 2201 TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG 2251 TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC 2301 TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC 2351 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG 2401 CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG 2451 ACGCGGATGA ACAGGCAGAG CTCTTACTGT CATGCCATCC GTATGTTTAA 2501 ACACAATAAA GTCTTAAACT GAACAAAATA GATCTAAACT ATGACAATAA 2551 AGTCTTAAAC TAGACAGAAT AGTTGTAAAC TGAAATCAGT CCAGTTATGC 2601 TGTGAAAAAG CATACTGGAC TTTTGTTATG GCTAAAGCAA ACTCTTCATT 2651 TTCTGAAGTG CAAATTGCCC GTCGTATTAA AGAGGGGCGT GGGGTCGACG 2701 CGGCCGCTAA CTATAACGGT CCTAAGGTAG CGAGTTTGGC GCGCCAAACG 2751 ATATCGGATC CAGGCGTTTA AGGGCACCAA TAACTGCCTT AAAAAAATTA 2801 CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC 2851 TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 2901 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC 2951 GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA 3001 AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 3051 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA 3101 TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG 3151 AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 3201 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ATTCCGGATG 3251 AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT 3301 GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 3351 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC 3401 TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT 3451 TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT 3501 ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC 3551 GTGCCGATCA ACGTCTCATT TTCGCCAAAA GTTGGCCCAG GGCTTCCCGG 3601 TATCAACAGG GACACCAGGA TTTATTTATT CTGCGAAGTG ATCTTCCGTC 3651 ACAGGTATGG ATCCGATATC ATTTAAATCT AGGGATAACA GGGTAATACT 3701 AGTGTCGACC AACCAGATAA GTGAAATCTA GTTCCAAACT ATTTTGTCAT 3751 TTTTAATTTT CGTATTAGCT TACGACGCTA CACCCAGTTC CCATCTATTT 3801 TGTCACTCTT CCCTAAATAA TCCTTAAAAA CTCCATTTCC ACCCCTCCCA 3851 GTTCCCAACT TTATTGTGTT TAAACACCGT AAGATGCTTT TCAGTGACT
References
- ↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
- ↑ Craig, NL (1996) Transposon Tn7. Curr. Top. Microbiol. Immunol. 204 27-48 PubMed
- ↑ Stellwagen, AE & Craig, NL (1997) Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7. Genetics 145 573-85 PubMed
- ↑ Biery, MC et al. (2000) A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucleic Acids Res. 28 1067-77 PubMed
- ↑ Metcalf, WW et al. (1994) Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138 1-7 PubMed
- ↑ Kolter, R et al. (1978) Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15 1199-208 PubMed
