View source for pBiEx-1

==At a Glance ==
[[Image:PBIEX-1.jpg|thumb|250px|right|Image of pBiEx-1. [http://www.merckbiosciences.com/docs/docs/PROT/TB354-000.pdf Novagen]]]
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!{{table heading style}} |Type
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Expression
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!{{table heading style}} |Origin of Replication
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pUC
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!{{table heading style}} |Copy #
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High
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!{{table heading style}} |Markers
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Ampicillin
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!{{table heading style}} |Link to Sequence
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[http://www.merckbiosciences.com/docs/NDIS/71234-000.html Novagen]
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!{{table heading style}} |Features
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hr5 enhancer <br>IE1 promoter <br>IE1 transcription start <br>T7 promoter <br>T7 transcription start <br>His•Tag® coding sequence <br>S•Tag™coding sequence <br>Multiple cloning sites (Bgl II–Dra III) <br>HSV•Tag® coding sequence <br>T7 terminator <br>IE1 terminator <br>lacI coding sequence <br>bla (Ap) coding sequence <br>pUC origin
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!{{table heading style}} |Accession Number
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==Notes==
The vectors feature the same hr5 enhancer and the ie1 (immediate early) promoter to direct expression in insect cells as the pIEx™ Vectors, and also allow for expression in E. coli by the tightly controlled T7lac promoter.

==History ==
The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.

==Sources ==
[http://www.emdbiosciences.com/product/71234 Novagen]

==References==
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<references/>

==Sequence ==
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat 
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca 
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg 
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata 
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac 
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg 
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa 
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac 
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg 
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg 
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag 
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg 
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga 
      781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta 
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg 
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca 
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt 
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg 
     1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct 
     1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcacaccat caccaccatc 
     1201 actcttctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgccac 
     1261 cgccttctgg tctggtcccc cggggcagcg caggttctgg tacgattgat gacgacgaca 
     1321 agatctctgg cccgggctcg gatcccaatt gggagctcgt gtacacggcg cgcctgcagg 
     1381 tcgacggtac cggttcgaag cttgcggccg cacagctgta tacacgtgca agccagccag 
     1441 aactcgcccc ggaagacccc gaggatctcg agcactaagt gattaacctc agccgaaagg 
     1501 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta 
     1561 aacgggtctt gaggggtttt ttgctgaaag gaggaactat cctcaggtta tacatatatt 
     1621 ttgaatttaa ttaattatac atatatttta tattattttt gtcttttatt atcgaggggc 
     1681 cgttgttggt gtggggtttt gcatagaaat aacaatggga gttggcgacg ttgctgcgcc 
     1741 aacaccacct cccttccctc ctttcatcat gtatctgtag ataaaataaa atattaaacc 
     1801 taaaaacaag accgcgccta tcaacaaaat gataggcatt aacttgccgc tgacgctgtc 
     1861 actaacgttg gacgatttgc cgactaaacc ttcatcgccc agtaaccaat ctagacgtca 
     1921 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca 
     1981 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac 
     2041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     2101 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg 
     2161 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg 
     2221 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat 
     2281 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg 
     2341 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca 
     2401 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc 
     2461 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag 
     2521 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc 
     2581 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa 
     2641 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg 
     2701 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca 
     2761 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt 
     2821 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg 
     2881 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac 
     2941 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc 
     3001 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa 
     3061 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca 
     3121 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg 
     3181 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa 
     3241 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatggac 
     3301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     3361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     3421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     3481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     3541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     3601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     3661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     3721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     3781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     3841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     3901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     3961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     4021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     4081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     4141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     4201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     4261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     4321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 
     4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     4801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     4861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     4921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     4981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     5041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     5101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 
     5161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 
     5221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
     5281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
     5341 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
     5401 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
     5461 ttacgaattc ccggg 



[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:IE1 Promoter]][[Category:HSV Tag]][[Category:His Tag]][[Category:S Tag]][[Category:Ampicillin Marker Plasmids]][[Category:hr5 Enhancer]]
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