View source for pBiEx-3

==At a Glance ==
[[Image:PBIEX-3.jpg|thumb|250px|right|Image of pBiEx-3. [http://www.merckbiosciences.com/docs/docs/PROT/TB355.pdf Novagen]]]
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!{{table heading style}} |Type
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Expression
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!{{table heading style}} |Origin of Replication
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pUC
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!{{table heading style}} |Copy #
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High
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!{{table heading style}} |Markers
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Ampicillin
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!{{table heading style}} |Link to Sequence
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[http://www.merckbiosciences.com/docs/NDIS/71232-000.html Novagen]
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!{{table heading style}} |Features
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hr5 enhancer <br>IE1 promoter <br>IE1 transcription start <br>T7 promoter <br>T7 transcription start <br>Multiple cloning sites (Nco I–Dra III) <br>S•Tag™coding sequence <br>His•Tag® coding sequence <br>T7 terminator <br>IE1 terminator <br>lacI coding sequence <br>bla (Ap) coding sequence <br>pUC origin
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!{{table heading style}} |Accession Number
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===Notes===

==History ==
The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.

==Sources ==
[http://www.emdbiosciences.com/product/71232 Novagen]

==References==
{{RefHelp}}
<references/>

==Sequence ==
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat 
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca 
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg 
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata 
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac 
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg 
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa 
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac 
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg 
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg 
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag 
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg 
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga 
      781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta 
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg 
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca 
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt 
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg 
     1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct 
     1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcgtta acacgtcaag 
     1201 agctcgcgga tcccaattgg cagatctcgg cgcgcctgca ggtcgacggt accggttcga 
     1261 agcttgcggc cgcatcttct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca 
     1321 tggactcgcc accgccttct ggcctcgagc accaccatca ccatcaccat cactaagtga 
     1381 ttaacctcag ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat 
     1441 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatcc 
     1501 tcaggttata catatatttt gaatttaatt aattatacat atattttata ttatttttgt 
     1561 cttttattat cgaggggccg ttgttggtgt ggggttttgc atagaaataa caatgggagt 
     1621 tggcgacgtt gctgcgccaa caccacctcc cttccctcct ttcatcatgt atctgtagat 
     1681 aaaataaaat attaaaccta aaaacaagac cgcgcctatc aacaaaatga taggcattaa 
     1741 cttgccgctg acgctgtcac taacgttgga cgatttgccg actaaacctt catcgcccag 
     1801 taaccaatct agacgtcacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt 
     1861 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat 
     1921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     1981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt 
     2041 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt 
     2101 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt 
     2161 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc 
     2221 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg 
     2281 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga 
     2341 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga 
     2401 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc 
     2461 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta 
     2521 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat 
     2581 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga 
     2641 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag 
     2701 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg 
     2761 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca 
     2821 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa 
     2881 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc 
     2941 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat 
     3001 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc 
     3061 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg 
     3121 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc 
     3181 aaggaatggt gcatggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     3241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     3301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     3361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     3421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     3481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     3541 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     3601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     3661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     3721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     3781 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     3841 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     3901 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     3961 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     4021 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     4081 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     4141 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     4201 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     4261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     4321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     4441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     4501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     4561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     4621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     4681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     4741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     4801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     4861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     4921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     4981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
     5041 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 
     5101 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 
     5161 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
     5221 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
     5281 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
     5341 aaacagctat gaccatgatt acgaattccc ggg



[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:IE1 Promoter]][[Category:His Tag]][[Category:S Tag]][[Category:Ampicillin Marker Plasmids]][[Category:hr5 Enhancer]]
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