View source for pGEMEX-1

==At a Glance ==
[[Image:#PGEMEX-1.jpg|thumb|400px|right|pGEMEX-1, Image provided by [http://www.promega.com/figures/popup.asp?fn=0309va Promega]]]
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!{{table heading style}} |Type
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Expression
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!{{table heading style}} |Origin of Replication
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!{{table heading style}} |Copy #
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!{{table heading style}} |Markers
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Ampicillin
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!{{table heading style}} |Link to Sequence
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=8216952 PubMed]
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!{{table heading style}} |Features
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HindIII, NsiI,  SphI,  NotI,  ApaI,  XhoI,  BamHI,  SalI, EcoRI,  SacI,  SfiI<br>
T7 RNA polymerase transcription initiation site<br>
SP6 RNA polymerase transcription initiation site<br>
T3 RNA polymerase transcriptioninitation site<br>
T7 RNA polymerase promoter <br>
SP6 RNA polymerase promoter<br>
T3 RNA polymerase promoter <br>
Multiple cloning region<br>	                               
T7 gene 10 start codon<br>                               
T7 terminator region<br>                    
Beta-lactamase (Ampr) coding region<br>                  
phage f1 region	
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!{{table heading style}} |Accession Number
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X65317
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==Notes==
Special usage restrictions<br>
The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/vectors/pgemex1.txt Promega]

==References==
{{RefHelp}}
<references/>

==Sequence ==
        1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
       61 aattcgagct cggccgactt ggccttccct ttagtgaggg ttaataaact tggccaattc
      121 gatccggtac cagcaccacc agcggtgagg tgcggaactt ctacaacctc aaagcccata
      181 acgttgcgga tagaaccctt ctcagggtca atcagagcag cgtagtttgc tgcgttcggc
      241 atcagtgctg ccagaatcgc agagtagcta tctgggtcac agtagaacac acggtcagca
      301 gccggaacat agttcttggt cagagccgca cgagccttag tcagagccgc aataatctcc
      361 ttacccagcg caacttggtc ggtaagtgcg gccttgttct gagtggtctc aattacggta
      421 gcagtaccta agccctcgat gttctcatta tatttgcttt ccacgttaca cagaccggca
      481 atctcagcca gaaccgcacc atccgcagcc atcgccagag attcacccaa ctgagaggta
      541 tactcagagc gaacgtcgta gtggttcatc gcgtcctcaa tatcataaat cagaacgtca
      601 gccgtcagga gaccgtcaat ggtgattacc ttctcggtgt gtttgatgtc cttacgttta
      661 tcgtcgaggt tctcgcccgg agccagatac gctgcctgag tgcgacccag aacagggaac
      721 tgagcggatt taccgctgga gatggaacgt accatgtggc gagaagtggt cacggaggta
      781 cgagcgaacg cagtcaggac ttcaccgcca aataccttca agaacaacgc cagtttatct
      841 ccagcagcaa ctacaccttt accttggtta gtacccattt gctgtccacc agtcatgcta
      901 gccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
      961 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcccgg gctgcattaa
     1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2281 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2581 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2641 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3061 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3181 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3241 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3301 aggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3361 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3481 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3601 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3661 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3721 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
     3781 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagg ttatccggat
     3841 atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
     3901 gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca
     3961 gccggatcaa gctgggattt aggtgacact ata

 




[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:T3 Promoter]]
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