pGEMEX-1
At a Glance
pGEMEX-1, Image made with PlasMapper
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| Type |
Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
HindIII, NsiI, SphI, NotI, ApaI, XhoI, BamHI, SalI, EcoRI, SacI, SfiI |
| Accession Number | |
| edit table |
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Notes
Special usage restrictions
The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.
History
Designed for cloning, as templates for in vitro transcription and for production of ssDNA.
Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
61 aattcgagct cggccgactt ggccttccct ttagtgaggg ttaataaact tggccaattc
121 gatccggtac cagcaccacc agcggtgagg tgcggaactt ctacaacctc aaagcccata
181 acgttgcgga tagaaccctt ctcagggtca atcagagcag cgtagtttgc tgcgttcggc
241 atcagtgctg ccagaatcgc agagtagcta tctgggtcac agtagaacac acggtcagca
301 gccggaacat agttcttggt cagagccgca cgagccttag tcagagccgc aataatctcc
361 ttacccagcg caacttggtc ggtaagtgcg gccttgttct gagtggtctc aattacggta
421 gcagtaccta agccctcgat gttctcatta tatttgcttt ccacgttaca cagaccggca
481 atctcagcca gaaccgcacc atccgcagcc atcgccagag attcacccaa ctgagaggta
541 tactcagagc gaacgtcgta gtggttcatc gcgtcctcaa tatcataaat cagaacgtca
601 gccgtcagga gaccgtcaat ggtgattacc ttctcggtgt gtttgatgtc cttacgttta
661 tcgtcgaggt tctcgcccgg agccagatac gctgcctgag tgcgacccag aacagggaac
721 tgagcggatt taccgctgga gatggaacgt accatgtggc gagaagtggt cacggaggta
781 cgagcgaacg cagtcaggac ttcaccgcca aataccttca agaacaacgc cagtttatct
841 ccagcagcaa ctacaccttt accttggtta gtacccattt gctgtccacc agtcatgcta
901 gccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
961 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcccgg gctgcattaa
1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
1081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
1141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
1201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
1261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
1321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
1381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
1441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
1501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
1561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
1621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
1681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
1741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
1801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
1861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
1921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
1981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
2041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
2101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
2161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
2221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
2281 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
2341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
2401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
2461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
2521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
2581 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
2641 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
2701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
2761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
2821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
2881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
2941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
3001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
3061 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
3121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
3181 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
3241 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
3301 aggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3361 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
3421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3481 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
3541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3601 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
3661 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
3721 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
3781 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagg ttatccggat
3841 atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
3901 gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca
3961 gccggatcaa gctgggattt aggtgacact ata
