pWEB-TNC
At a Glance
<protect>
Type |
Cloning |
---|---|
Origin of Replication |
col E1 |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
Facilitates rapid and efficient construction of unbiased primary cosmid libraries. Can be used the same way as pWEB vector, but offers extra features such as use for subsequent generation of a random unidirectional deletion library from any chosen cosmid clone. |
Accession Number | |
edit table |
</protect>
Notes
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaattcgcgg ccgccagggt tttcccagtc acgacggatc ccgggatcct ctccctatag 61 tgagtcgtat tatgcggccg cgaattctca tgtttgaccg cttatcatcg ataagcttta 121 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa 181 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt 241 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca 301 ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg 361 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac 421 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atctgcctcg ctggcctgcc 481 gcagttcttc aacctcccgg cgcagctttt cgttctcaat ttcagcatcc ctttcggcat 541 accattttat gacggcggca gagtcataaa gcacctcatt acccttgcca ccgcctcgca 601 gaacgggcat tccctgttcc tgccagttct gaatggtacg gatactcgca ccgaaaatgt 661 cagccagctg ctttttgttg acttccattg ttcattccac ggacaaaaac agagaaagga 721 aacgacagag gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt 781 attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa 841 ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacaaggcgg atcgccggaa 901 aggacccgca aatgataata attatcaatt gcatactatc gacggcactg ctgccagata 961 acaccaccgg ggaaacattc catcatgatg gccgtgcgga cataggaagc cagttcatcc 1021 atcgctttct tgtctgctgc catttgcttt gtgacatcca gcgccgcaca ttcagcagcg 1081 tttttcagcg cgttttcgat caacgtttca atgttggtat caacaccagg tttaactttg 1141 aacttatcgg cactgacggt taccttgttc tgcgctggct catcacgctg gataccaagg 1201 ctgatgttgt agatattggt caccggctga ggtgtttcga ttgccgctgc gtggatagca 1261 ccatttgcga tagcggcgtc cttgatgaat gacactccat tgcgaataag ttcgaaggag 1321 acggtgtcac gaatgcgctg gtccagctcg tcgattgcct tttgtgcagc agaggtatca 1381 atctcaacgc caagcgtcat cgaagcgcaa tattgctgct caccaaaacg cgtattgacc 1441 aggtgttcaa cggcaaattt ctgcccttct gatgtcagaa aggtaaagtg attttctttc 1501 tggtattcag ttgctgtgtg tctggtttca gcaaaaccaa gctcgcgcaa ttcggctgtg 1561 ccagatttag aaggcagatc accagacagc aacgcgccac ggaaaaacag cgcatacaga 1621 acatccgtcg ccgcgccgga caacgtgata attttatgac ccatgattta tttcctttta 1681 gacgtgagcc tgtcgcacag caaagccgcc gaaagttctc actcactcaa gatgtgtata 1741 agagacagtc gaaacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca 1801 ccgctgtccg gtagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 1861 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgcccaa cagtcccccg 1921 gccacggggc ctgccaccat acccacgccg aaacaagcgc cctgcaccat tatgttccgg 1981 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag 2041 cgctaaccgt ttttatcagg ctctgggagg cagaataaat gatcatatcg tcaattatta 2101 cctccacggg gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac 2161 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac 2221 cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt 2281 attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact gccttaaaaa 2341 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg 2401 acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg 2461 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg 2521 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata 2581 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc 2641 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac 2701 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc 2761 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg 2821 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta 2881 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa 2941 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc 3001 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat 3061 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc 3121 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg 3181 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac 3241 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag 3301 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca 3361 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa 3421 gttcgactgt ctcttataca catcttgagt gagtgagaac ttgtttattg cagcttataa 3481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgacgggt 3601 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 3661 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 3721 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 3781 gcggaagtca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccagcaaa aggccaggaa 3961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 4021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 4081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 4141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 4201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 4261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 4321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 4381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 4441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 4501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 4561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 4621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 4681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 4741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 5041 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 5101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 5161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 5221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 5281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 5341 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 5401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 5461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 5521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 5581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 5641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 5701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 5761 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aa