pWEB-TNC

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At a Glance

pWEB-TNC, Image made with PlasMapper

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Type

Cloning

Origin of Replication

col E1

Copy #
Markers

Ampicillin

Link to Sequence

PubMed

Features

Facilitates rapid and efficient construction of unbiased primary cosmid libraries. Can be used the same way as pWEB vector, but offers extra features such as use for subsequent generation of a random unidirectional deletion library from any chosen cosmid clone.

Accession Number

EU140755.1

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Notes

History

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Sources

Epicentre

References

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Sequence

        1 gaattcgcgg ccgccagggt tttcccagtc acgacggatc ccgggatcct ctccctatag
       61 tgagtcgtat tatgcggccg cgaattctca tgtttgaccg cttatcatcg ataagcttta
      121 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
      181 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
      241 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
      301 ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
      361 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
      421 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atctgcctcg ctggcctgcc
      481 gcagttcttc aacctcccgg cgcagctttt cgttctcaat ttcagcatcc ctttcggcat
      541 accattttat gacggcggca gagtcataaa gcacctcatt acccttgcca ccgcctcgca
      601 gaacgggcat tccctgttcc tgccagttct gaatggtacg gatactcgca ccgaaaatgt
      661 cagccagctg ctttttgttg acttccattg ttcattccac ggacaaaaac agagaaagga
      721 aacgacagag gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt
      781 attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa
      841 ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacaaggcgg atcgccggaa
      901 aggacccgca aatgataata attatcaatt gcatactatc gacggcactg ctgccagata
      961 acaccaccgg ggaaacattc catcatgatg gccgtgcgga cataggaagc cagttcatcc
     1021 atcgctttct tgtctgctgc catttgcttt gtgacatcca gcgccgcaca ttcagcagcg
     1081 tttttcagcg cgttttcgat caacgtttca atgttggtat caacaccagg tttaactttg
     1141 aacttatcgg cactgacggt taccttgttc tgcgctggct catcacgctg gataccaagg
     1201 ctgatgttgt agatattggt caccggctga ggtgtttcga ttgccgctgc gtggatagca
     1261 ccatttgcga tagcggcgtc cttgatgaat gacactccat tgcgaataag ttcgaaggag
     1321 acggtgtcac gaatgcgctg gtccagctcg tcgattgcct tttgtgcagc agaggtatca
     1381 atctcaacgc caagcgtcat cgaagcgcaa tattgctgct caccaaaacg cgtattgacc
     1441 aggtgttcaa cggcaaattt ctgcccttct gatgtcagaa aggtaaagtg attttctttc
     1501 tggtattcag ttgctgtgtg tctggtttca gcaaaaccaa gctcgcgcaa ttcggctgtg
     1561 ccagatttag aaggcagatc accagacagc aacgcgccac ggaaaaacag cgcatacaga
     1621 acatccgtcg ccgcgccgga caacgtgata attttatgac ccatgattta tttcctttta
     1681 gacgtgagcc tgtcgcacag caaagccgcc gaaagttctc actcactcaa gatgtgtata
     1741 agagacagtc gaaacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca
     1801 ccgctgtccg gtagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     1861 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgcccaa cagtcccccg
     1921 gccacggggc ctgccaccat acccacgccg aaacaagcgc cctgcaccat tatgttccgg
     1981 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
     2041 cgctaaccgt ttttatcagg ctctgggagg cagaataaat gatcatatcg tcaattatta
     2101 cctccacggg gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac
     2161 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
     2221 cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt
     2281 attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact gccttaaaaa
     2341 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     2401 acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     2461 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     2521 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     2581 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     2641 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     2701 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     2761 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg
     2821 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     2881 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     2941 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     3001 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     3061 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     3121 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     3181 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
     3241 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     3301 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
     3361 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     3421 gttcgactgt ctcttataca catcttgagt gagtgagaac ttgtttattg cagcttataa
     3481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgacgggt
     3601 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
     3661 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
     3721 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
     3781 gcggaagtca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccagcaaa aggccaggaa
     3961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5041 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5341 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     5401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     5761 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aa