pWEB
At a Glance
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Type |
Cloning |
---|---|
Origin of Replication |
col E1, SV40 |
Copy # | |
Markers |
Ampicillin Neomycin/Kanamycin |
Link to Sequence | |
Features |
Facilitates rapid and efficient construction of unbiased primary cosmid libraries. |
Accession Number | |
edit table |
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Notes
History
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Sources
References
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Sequence
1 gaattcgcgg ccgccagggt tttcccagtc acgacggatc ccgggatcct ctccctatag 61 tgagtcgtat tatgcggccg cgaattctca tgtttgaccg cttatcatcg ataagcttta 121 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa 181 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt 241 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca 301 ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg 361 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac 421 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atctgcctcg ctggcctgcc 481 gcagttcttc aacctcccgg cgcagctttt cgttctcaat ttcagcatcc ctttcggcat 541 accattttat gacggcggca gagtcataaa gcacctcatt acccttgcca ccgcctcgca 601 gaacgggcat tccctgttcc tgccagttct gaatggtacg gatactcgca ccgaaaatgt 661 cagccagctg ctttttgttg acttccattg ttcattccac ggacaaaaac agagaaagga 721 aacgacagag gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt 781 attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa 841 ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacaaggcgg atcgccggaa 901 aggacccgca aatgataata attatcaatt gcatactatc gacggcactg ctgccagata 961 acaccaccgg ggaaacattc catcatgatg gccgtgcgga cataggaagc cagttcatcc 1021 atcgctttct tgtctgctgc catttgcttt gtgacatcca gcgccgcaca ttcagcagcg 1081 tttttcagcg cgttttcgat caacgtttca atgttggtat caacaccagg tttaactttg 1141 aacttatcgg cactgacggt taccttgttc tgcgctggct catcacgctg gataccaagg 1201 ctgatgttgt agatattggt caccggctga ggtgtttcga ttgccgctgc gtggatagca 1261 ccatttgcga tagcggcgtc cttgatgaat gacactccat tgcgaataag ttcgaaggag 1321 acggtgtcac gaatgcgctg gtccagctcg tcgattgcct tttgtgcagc agaggtatca 1381 atctcaacgc caagcgtcat cgaagcgcaa tattgctgct caccaaaacg cgtattgacc 1441 aggtgttcaa cggcaaattt ctgcccttct gatgtcagaa aggtaaagtg attttctttc 1501 tggtattcag ttgctgtgtg tctggtttca gcaaaaccaa gctcgcgcaa ttcggctgtg 1561 ccagatttag aaggcagatc accagacagc aacgcgccac ggaaaaacag cgcatacaga 1621 acatccgtcg ccgcgccgga caacgtgata attttatgac ccatgattta tttcctttta 1681 gacgtgagcc tgtcgcacag caaagccgcc gaaagttaac ggtttgccca ggctcacaac 1741 tgaaagactt tctacggtgt gcgcgtgcga tgcgcgtaga agactgattt atcaacctgt 1801 ctttatatca ggattcatta cctgactatt tgtgggtaaa gttcgtagtg cgctgatcgt 1861 gcaaaatgat tttagttggg aacagttcgc aactctgtcc cataaaaatc agcatattcc 1921 catctatccc atatccagcg cattgaccat cgggatactg aagggagatt ccatcatctc 1981 ttagaaagat caccatctct tttgtttcaa tttgcatata gctacctgga ggatttatga 2041 atacaaggat tttcatggac tattaccatg agattgattt tccatcttta ttcgcgagag 2101 cagtggaaag cgatgacgat gtgggtacta cattgcgcat tcacctactt tgtgagcgca 2161 tggtcgaagc atggatatgc gcatgctgtg actgccaaga tcctctacgc cggacgcatc 2221 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 2281 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 2341 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 2401 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 2461 cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg 2521 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc 2581 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc 2641 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc 2701 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg 2761 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc 2821 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg 2881 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc 2941 agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc 3001 acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg 3061 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg 3121 ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg 3181 cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc 3241 ggcgcatctc gggcagcgtt gggtcctgca gatccggctg tggaatgtgt gtcagttagg 3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3361 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3421 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 3481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 3541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 3601 cctaggcttt tgcaaaaagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa 3661 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc 3721 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc 3781 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa 3841 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct 3901 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga 3961 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 4021 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 4081 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 4141 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 4201 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 4261 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 4321 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 4381 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 4441 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 4501 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4561 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4621 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 4681 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4741 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 4801 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 4861 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 4921 tggagttctt cgcccaccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg 4981 ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc 5041 agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct 5101 ttggtccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta 5161 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa 5221 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag 5281 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt 5341 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta 5401 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt 5461 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac 5521 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat 5581 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct 5641 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg 5701 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc 5761 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 5821 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 5881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 5941 atcttatcat gtctggatct gacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg 6001 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac 6061 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg 6121 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct tcctcgctca 6181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 6241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6361 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6781 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 6961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7081 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7141 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7201 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7261 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7321 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7381 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 7441 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 7501 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7561 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7621 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7681 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 7741 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 7801 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 7861 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 7921 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8041 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8101 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 8161 gaggcccttt cgtcttcaa