pSP70
At a Glance
pSP70, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
SP6 promoter Xho I Pvu I Hind III EcoR I Cla I EcoR V Bgl II |
| Accession Number | |
| edit table |
</protect>
Notes
carries 2kb at the 5' end but does not extend past the SP70.[1]
History
pGEM-1 was subcloned with a 3.5kb insert from p2H3 to form pSP70.[1]
Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaactcgagc agctgaagct tgaattcatc gatgatatca gatctgccgg tctccctata
61 gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc
121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1021 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1081 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1141 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1201 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
1261 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1321 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1381 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1441 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1501 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1561 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1621 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1681 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1741 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1801 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
1861 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
1921 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
1981 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2041 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2101 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
2161 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
2221 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
2281 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga
2341 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
2401 atttaggtga cactata
