pSP65
At a Glance
pSP65, Image made with PlasMapper
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| Type |
Cloning |
|---|---|
| Origin of Replication |
pUC, M13 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
SP6 RNA polymerase promoter EcoR I Sac I Ava I Sma I Xba I BamH I Sal I Acc I Hinc II Pst I Hind III |
| Accession Number | |
| edit table |
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Notes
Transcription in vitro from SP6 promoter. Blue/white screening for recombinants is not possible with the pSP65 Vector.
History
The pSP64 and pSP65 Vectors are nearly identical, except for the orientation of their multiple cloning regions.
Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
61 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
121 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
181 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
241 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
901 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
1021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
1141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
1201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
1261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
1321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
1381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
1441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
1501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
1561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
1621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
1681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
1741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
1801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
1861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
1921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
1981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
2041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
2101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
2161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
2221 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
2281 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
2341 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
2401 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
2461 tgtactgaga gtgcaccata tcgacgctct cccttatgcg actcctgcat taggaagcag
2521 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
2581 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
2641 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
2701 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
2761 tggctagcga tgaccctgct gattggttcg ctgaccattt ccggggtgcg gaacggcgtt
2821 accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt acgagagaga
2881 tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgtaca tattgtcgtt
2941 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
3001 ctata
