pRSFDuet-1
At a Glance
At a Glance
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pRSFDuet-1 (section) Image made with PlasMapper
<protect>
| Type |
Coexpression |
|---|---|
| Origin of Replication |
RSF |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
2 T7 Promoters 2 lac Operators His Tag S Tag 2 MCS lacI coding sequence RBS |
| Accession Number |
|
| edit table |
</protect>
Notes
pRSFDuet-1 is designed for the coexpression of two target proteins. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs. The vector also carries the pRSF1030 replicon (also known as NTP1), lacI gene, and kanamycin resistance marker. Plasmids that share common origins of replication or antibiotic resistance genes should not be used for the coexpression of two proteins[1].
History
The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli to be used together or with existing pET, pCDF, pRSF, and pETcoco recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell[2][3]. Duet vectors are also designed with the option of producing native unfused proteins or fusions to His•Tag and S•Tag sequences for detection and purification of protein complexes.
Sources
References
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Sequence
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt 601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt 661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt 781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa 841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg 901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa 961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca 1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc 1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca 1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt 1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt 1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat 1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc 1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt 1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat 1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt 1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac 1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt 1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa 1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc 1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc 1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt 2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag 2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc 2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta 2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca 2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc 2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg 2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga 2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc 2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact 2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc 2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc 3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga 3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa 3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg 3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag 3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg 3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc 3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc 3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga 3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg 3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa 3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct 3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg 3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg 3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata
