pRSF-2 Ek/LIC
At a Glance
pRSF-2 Ek-LIC Image made with PlasMapper
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
RSF |
| Copy # |
High-level |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
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Notes
The pRSF-2 Ek/LIC Vector is a LIC-compatible derivative of pRSF-1b. This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pCDF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors. It contains a strong T7lac promoter and an amino-terminal His•Tag coding sequence followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification[1].
History
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Sources
References
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Sequence
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atggcacatc accaccacca tcacgtggat gacgacgaca agagtccggg
121 cttctcctca aatctcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca
181 catggactcg tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata
241 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
301 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
361 atagacataa gcggctattt aacgaccctg ccctgaaccg acgacaagct gacgaccggg
421 tctccgcaag tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
481 aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc
541 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
601 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
661 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
721 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
781 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
841 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
901 cggtcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
961 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
1021 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
1081 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
1141 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
1201 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
1261 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
1321 tgaatatggc tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
1381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg catgcagcgc
1441 tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg
1501 tcagctcact caaaagcggt aatacggtta tccacagaat caggggataa agccggaaag
1561 aacatgtgag caaaaagcaa agcaccggaa gaagccaacg ccgcaggcgt ttttccatag
1621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc
1681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
1741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1801 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccattg gtaactgatt
1981 tagaggactt tgtcttgaag ttatgcacct gttaaggcta aactgaaaga acagattttg
2041 gtgagtgcgg tcctccaacc cacttacctt ggttcaaaga gttggtagct cagcgaacct
2101 tgagaaaacc accgttggta gcggtggttt ttctttattt atgagatgat gaatcaatcg
2161 gtctatcaag tcaacgaaca gctattccgt tactctagat ttcagtgcaa tttatctctt
2221 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
2281 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
2341 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
2401 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
2461 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
2521 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
2581 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
2641 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
2701 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
2761 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
2821 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
2881 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
2941 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
3001 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
3061 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
3121 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
3181 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
3241 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
3301 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
3361 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
3421 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
3481 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
3541 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga
3601 ctcactata
