pNEBR-X1Hygro
At a Glance
pNEBR-X1Hygro, Image provided by NEB
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
pUC19 |
| Copy # | |
| Markers |
Ampicillin, Hygromicin |
| Link to Sequence | |
| Features |
SV40 TT region |
| Accession Number |
|
| edit table |
</protect>
Notes
Translation initiation signals must be included in the cloned insert DNA as none are present in this vector. A standard plasmid purification procedure is used to derive pNEBR-X1 Vector from E. coli strain ER2272.
History
pNEBR-X1Hygro is a plasmid cloning vector that can replicate in E.coli and capable of stable transfection of mammalian cells. It is designed for inducible expression of recombinant proteins in mammalian cells.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 GTTCTAGAGG ATCTTTGTGA AGGAACCTTA CTTCTGTGGT GTGACATAAT
51 TGGACAAACT ACCTACAGAG ATTTAAAGCT CTAAGGTAAA TATAAAATTT
101 TTAAGTGTAT AATGTGTTAA ACTACTGATT CTAATTGTTT GTGTATTTTA
151 GATTCCAACC TATGGAACTT ATGAATGGGA GCAGTGGTGG AATGCCTTTA
201 ATGAGGAAAA CCTGTTTTGC TCAGAAGAAA TGCCATCTAG TGATGATGAG
251 GCTACTGCTG ACTCTCAACA TTCTACTCTC AAAAGAAGAG AAAGGTAGAG
301 ACCCAAGGAC TTTCCTTCAG AATTGCTAAG TTTTTTGAGT CATGCTGTGT
351 TTAGTAATAG AACTCTTGCT TGCTTTGCTA TTTACAACCA CAAAGGAAAA
401 AGCTGCACTG CTATACAAGA AAATTATGGA AAAATATTCT GTAACCTTTA
451 TAAGTAGGCA TAACAGTTAT AATCATAACA TACTGTTTTT TCTTACTCCA
501 CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA AATTGTGTAC
551 CTTTAGCTTT TTAATTTGTA AAGGGGTTAA TAAGGAATAT TTGATGTATA
601 GTGCCTTGAC TAGAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT
651 ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA
701 TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
751 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
801 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT
851 GGATCAACTA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
901 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
951 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
1001 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
1051 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG
1101 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT
1151 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTCTCCAA TGATGAGCAC
1201 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC
1251 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
1301 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
1351 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC
1401 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC
1451 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
1501 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA
1551 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG
1601 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
1651 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
1701 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
1751 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
1801 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT
1851 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTACCC
1901 CGGTTGATAA TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT
1951 ATTCATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA
2001 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
2051 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT
2101 CCTTACGCAT CTGTGCGGTA TTTCACACCG CACTCTCTCA TATGCGCCAG
2151 AAATCCGCGC GGTGGTTTTT GGGGGTCGGG GGTGTTTGGC AGCCACAGAC
2201 GCCCGGTGTT CGTGTCGCGC CAGTACATGC GGTCCATGCC CAGGCCATCC
2251 AAAAACCATG GGTCTGTCTG CTCAGTCCAG TCGTGGACCT GACCCCACGC
2301 AACGCCCAAA ATAATAACCC CCACGAACCA TAAACCATTC CCCATGGGGG
2351 ACCCCGTCCC TAACCCACGG GGCCAGTGGC TATGGCAGGG CCTGCCGCCC
2401 CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT
2451 GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG
2501 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA
2551 ACGACCCAAC ACCCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC
2601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC
2651 ATCTCCCGAT CCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG
2701 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT
2751 GTGTACGCCC GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA
2801 CCCTGCGCCC AAGCTGCATC ATCGAAATTG CCGTCAACCA AGCTCTGATA
2851 GAGTTGGTCA AGACCAATGC GGAGCATATA CGCCCGGAGC CGCGGCGATC
2901 CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG CTGCTCCATA
2951 CAAGCCAACC ACGGCCTCCA GAAGAAGATG TTGGCGACCT CGTATTGGGA
3001 ATCCCCGAAC ATCGCCTCGC TCCAGTCAAT GACCGCTGTT ATGCGGCCAT
3051 TGTCCGTCAG GACATTGTTG GAGCCGAAAT CCGCGTGCAC GAGGTGCCGG
3101 ACTTCGGGGC AGTCCTCGGC CCAAAGCATC AGCTCATCGA GAGCCTGCGC
3151 GACGGACGCA CTGACGGTGT CGTCCATCAC AGTTTGCCAG TGATACACAT
3201 GGGGATCAGC AATCGCGCAT ATGAAATCAC GCCATGTAGT GTATTGACCG
3251 ATTCCTTGCG GTCCGAATGG GCCGAACCCG CTCGTCTGGC TAAGATCGGC
3301 CGCAGCGATC GCATCCATGG CCTCCGCGAC CGGCTGCAGA ACAGCGGGCA
3351 GTTCGGTTTC AGGCAGGTCT TGCAACGTGA CACCCTGTGC ACGGCGGGAG
3401 ATGCAATAGG TCAGGCTCTC GCTGAATTCC CCAATGTCAA GCACTTCCGG
3451 AATCGGGAGC GCGGCCGATG CAAAGTGCCG ATAAACATAA CGATCTTTGT
3501 AGAAACCATC GGCGCAGCTA TTTACCCGCA GGACATATCC ACGCCCTCCT
3551 ACATCGAAGC TGAAAGCACG AGATTCTTCG CCCTCCGAGA GCTGCATCAG
3601 GTCGGAGACG CTGTCGAACT TTTCGATCAG AAACTTCTCG ACAGACGTCG
3651 CGGTGAGTTC AGGCTTTTTC ATATCAAGCT GATCTGCGGC ACGCTGTTGA
3701 CGCTGTTAAG CGGGTCGCTG CAGGGTCGCT CGGTGTTCGA GGCCACACGC
3751 GTCACCTTAA TATGCGAAGT GGACCTCGGA CCGCGCCGCC CCGACTGCAT
3801 CTGCGTGTTC GAATTCGCCA ATGACAAGAC GCTGGGCGGG GTTTGTGTCA
3851 TCATAGAACT AAAGACATGC AAATATATTT CTTCAGGGGA CACCGCCAGC
3901 AAACGCGAGC AACGGGCCAC GGGGATGAAG CAGTAAAATT GTAAACGTTA
3951 ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT
4001 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGCC
4051 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA
4101 AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT
4151 GGCCCACTAC GTGAACCATC ACCCAAATCA AGTTTTTTGG GGTCGAGGTG
4201 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT
4251 GACGGGGAAA GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG
4301 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC
4351 ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTAAA AGGATCTAGG
4401 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT
4451 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
4501 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
4551 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
4601 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
4651 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
4701 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT
4751 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
4801 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
4851 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC
4901 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
4951 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
5001 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT
5051 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
5101 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTATC
5151 AGCCATATCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA
5201 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTAACTTGT
5251 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5301 ACAAATAAAG CATTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
5351 TCAATGTATC TTATCATGTC TGGATCAGCC ATATCACATT TGTAGAGGTT
5401 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA
5451 AATGAATGCA ATTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
5501 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
5551 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
5601 CCATGTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
5651 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC
5701 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTACG GGCGGAGTAC
5751 TGTCCTCCGA GCGGAGTACT GTCCTCCGAG CGGAGTACTG TCCTCCGAGC
5801 GGAGTACTGT CCTCCGAGCG GAGTACTGTC CTCCGAGCGG AGACTCTAGA
5851 GGGTATATAA TGGGGGCCGT CAGCTACTAC CAGAGCTCAT GGGCCCGTGC
5901 AATTGAAGCC GGCTGGCGCC AAGCTTCTCT GCAGGATATC TGGATCCACG
5951 AATTCTTAAT TAAGGCCGGC CTCGAGCGGC CGCTAGCGAT CGCGGCGCGC
6001 CACGCGTCTC CGGATGTACA GGCATGCGTC GACCCTCTAG TCAAGGCCTA
6051 TAGTGAGTCG TATTACGGAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
6101 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
6151 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA
6201 GTTGCGCAGC CTGAATGGCG AATGGCGCTT CGCTTGGTAA TAAAGCCCGC
6251 TTCGGCGGGC TTTTTTTT
- ↑ Palli, SR et al. (2003) Improved ecdysone receptor-based inducible gene regulation system. Eur. J. Biochem. 270 1308-15 PubMed
- ↑ Kumar, MB et al. (2004) Highly flexible ligand binding pocket of ecdysone receptor: a single amino acid change leads to discrimination between two groups of nonsteroidal ecdysone agonists. J. Biol. Chem. 279 27211-8 PubMed
- ↑ Verhaegent, M & Christopoulos, TK (2002) Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal. Chem. 74 4378-85 PubMed
