pNEBR-X1
At a Glance
_
pNEBR-X1, Image provided by NEB
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
M13, pUC19 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
SV40 polyA |
| Accession Number |
|
| edit table |
</protect>
Notes
pNEBR-X1 is used to clone and express a gene of interest in mammalian cells as part of the RheoSwitch® Mammalian Inducible Expression System. When used in conjunction with pNEBR-R1 Regulator Plasmid, expression of a cloned gene is controlled by the addition of RSL1. Its source is the E.coli strain ER 2272 by a standard plasmid purification procedure.
History
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Sources
References
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Sequence
1 GTTCTAGAGG ATCTTTGTGA AGGAACCTTA CTTCTGTGGT GTGACATAAT
51 TGGACAAACT ACCTACAGAG ATTTAAAGCT CTAAGGTAAA TATAAAATTT
101 TTAAGTGTAT AATGTGTTAA ACTACTGATT CTAATTGTTT GTGTATTTTA
151 GATTCCAACC TATGGAACTT ATGAATGGGA GCAGTGGTGG AATGCCTTTA
201 ATGAGGAAAA CCTGTTTTGC TCAGAAGAAA TGCCATCTAG TGATGATGAG
251 GCTACTGCTG ACTCTCAACA TTCTACTCTC AAAAGAAGAG AAAGGTAGAG
301 ACCCAAGGAC TTTCCTTCAG AATTGCTAAG TTTTTTGAGT CATGCTGTGT
351 TTAGTAATAG AACTCTTGCT TGCTTTGCTA TTTACAACCA CAAAGGAAAA
401 AGCTGCACTG CTATACAAGA AAATTATGGA AAAATATTCT GTAACCTTTA
451 TAAGTAGGCA TAACAGTTAT AATCATAACA TACTGTTTTT TCTTACTCCA
501 CACAGGCATA GAGTGTCTGC TATTAATAAC TATGCTCAAA AATTGTGTAC
551 CTTTAGCTTT TTAATTTGTA AAGGGGTTAA TAAGGAATAT TTGATGTATA
601 GTGCCTTGAC TAGAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT
651 ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA
701 TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
751 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
801 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT
851 GGATCAACTA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
901 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
951 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
1001 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
1051 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG
1101 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT
1151 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTCTCCAA TGATGAGCAC
1201 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC
1251 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
1301 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
1351 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC
1401 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC
1451 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
1501 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA
1551 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG
1601 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
1651 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
1701 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
1751 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
1801 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT
1851 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTACCC
1901 CGGTTGATAA TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT
1951 ATTTAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG
2001 TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
2051 ATAAATCAAA AGAATAGCCC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG
2101 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA
2151 AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA
2201 GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG
2251 AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CGAACGTGGC GAGAAAGGAA
2301 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT
2351 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
2401 GCGCGTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA
2451 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
2501 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
2551 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
2601 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
2651 TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
2701 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
2751 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
2801 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
2851 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
2901 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
2951 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
3001 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
3051 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
3101 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
3151 CCTTTTGCTC ACATGTATCA GCCATATCAC ATTTGTAGAG GTTTTACTTG
3201 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT
3251 GCAATTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
3301 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTCACT GCATTCTAGT
3351 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAGCCA
3401 TATCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC
3451 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTAA CTTGTTTATT
3501 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA
3551 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
3601 ATGTATCTTA TCATGTCTGC CATGTAATGT GAGTTAGCTC ACTCATTAGG
3651 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT
3701 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC
3751 CAAGCTACGG GCGGAGTACT GTCCTCCGAG CGGAGTACTG TCCTCCGAGC
3801 GGAGTACTGT CCTCCGAGCG GAGTACTGTC CTCCGAGCGG AGTACTGTCC
3851 TCCGAGCGGA GACTCTAGAG GGTATATAAT GGGGGCCGTC AGCTACTACC
3901 AGAGCTCATG GGCCCGTGCA ATTGAAGCCG GCTGGCGCCA AGCTTCTCTG
3951 CAGGATATCT GGATCCACGA ATTCTTAATT AAGGCCGGCC TCGAGCGGCC
4001 GCTAGCGATC GCGGCGCGCC ACGCGTCTCC GGATGTACAG GCATGCGTCG
4051 ACCCTCTAGT CAAGGCCTAT AGTGAGTCGT ATTACGGACT GGCCGTCGTT
4101 TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT
4151 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
4201 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCTTC
4251 GCTTGGTAAT AAAGCCCGCT TCGGCGGGCT TTTTTTT
