pNEBR-R1

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At a Glance

pNEBR-R1, Image provided by NEB

<protect>

Type

Regulator

Origin of Replication

pUC19

Copy #
Markers

Ampicillin, Neomycin

Link to Sequence

NEB

Features

UbB, UbC Promoters
SV40 polyA
RheoActivator CDS
RheoReceptor CDS

Accession Number


</protect>

Notes

pNEBR-R1 is designed to control gene expression in mammalian cells as part of the RheoSwitch® Inducible Gene Expression System. It can be linearized using one of the unique restriction enzyme sites shown on map. The plasmid expresses an engineered nuclear receptor heterodimer consisting of two proteins, RheoReceptor-1 and RheoActivator. Its source is the E.coli strain ER2272 by a standard plasmid purification procedure.

History

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Sources

New England Biolabs

References

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Sequence

      1  CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     51  ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 
    101  CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
    151  CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
    201  CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 
    251  GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
    301  ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
    351  TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 
    401  AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA 
    451  CGCTTACAAT TTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC 
    501  GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
    551  GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 
    601  TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT 
    651  TGGGTACGTA GGCGGCCCGC CAGAAATCCG CGCGGTGGTT TTTGGGGGTC 
    701  GGGGGTGTTT GGCAGCCACA GACGCCCGGT GTTCGTGTCG CGCCAGTACA 
    751  TGCGGTCCAT GCCCAGGCCA TCCAAAAACC ATGGGTCTGT CTGCTCAGTC 
    801  CAGTCGTGGA CCTGACCCCA CGCAACGCCC AAAAGAATAA CCCCCACGAA 
    851  CCATAAACCA TTCCCCATGG GGGACCCCGT CCCTAACCCA CGGGGCCCGT 
    901  GGCTATGGCG GGCTTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC
    951  ACCCGAACTT GGGGGTTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT 
   1001  GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC 
   1051  CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCCGTGC GTTTTATTCT 
   1101  GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC 
   1151  CGTGTTTCAG TTAGCCTCCC CCATCTCCCT GCAGCCCGGG GGATCGAAAG 
   1201  GCGGCCGCCT AGAGATTCGT GGGGGACTCG AGGATAGGCG GCGGTTGGGT 
   1251  GTGCGACATG TCCGCCACAT CCCAGATCTC CTCGAGGAAA GGCGGCAGCT 
   1301  TTCTGTTCTT GAGCTTGAGG GAGATGCACA TGTTGGAGTT TTGCATGCCG 
   1351  AGCGTGCGTA GCTCAGAGAG GATTGAGAGG ATCTTGCCGT ATATGACGGA 
   1401  CGAACGCGCC GACCCGCTCA GCTGGTTCAG GATATAGATG CGGAGCGTAT 
   1451  TCAGGTAGTA CCGCTGGATT TCTTCCACCA GTTGCGGCTG CTCCAACCCT 
   1501  GGCCGGTCAG AAAAGATGAC GACAGCCGTG AGCAGCGCGT AATGGATGTT 
   1551  GTCCAACGCC ATAGAGTACA TGCACCGGCA GAAGTGCAGT AGATCCTCGA 
   1601  TGACCTCGGC CATGCCAGCC TTGCGGTAGT TGTCGCGAGT GTACGCTTGG 
   1651  TTGTTCGCGA ACAGAATACT GTCTGAGGCC GCATCGTATC GTCGCGCGAC 
   1701  TCGGAGCATC ATTACCTCAC TTGAGCAAGC CTTAAGCAGC GTAATTTGAT 
   1751  CAGGCTGCGA GATCTTGGCG AACCCTGGCA ATCCCTTCGC GAACTCCACG 
   1801  ATAAGTTGGA CCGTGAGGAT AGTCATCTCT GTGATCTGGC GGAAGGGAGT 
   1851  GTCAGACTCT TCGTTTTCAT CGTCCGCTTG CTGCCACGTC TGCGTAATCC 
   1901  TCTTCAAATC TTCATCAGAA GGCTGCTCGT ACCCGTCCTG GTACCAGATG 
   1951  AGCCTGGCGA TAAGGAACTG CTGGTTGGCT GTCAACTGGG GGATGTTTTT 
   2001  CTGCCGGTTT GTCTCCAACA GCTTGTCGGA GAGAAACCTT GGGACCACTT 
   2051  CGTGAATCCT TGCTGCTTCA GGAGGTGGAG GTTCACACTG CATAATGGGC 
   2101  GGCATGTGGT CGTCCACCGT CGTCGTGCTG ACAGGCAGTT TGTCCTTCTC 
   2151  CTTCTGTGCT TTCTTCTCTT TCCGCTTCAT GGCGCACTGA GTCTCGGGTA 
   2201  CTACGCACTC AGGCCGGATC CCCGGGAATT CCGGCGATAC AGTCAACTGT 
   2251  CTTTGACCTT TGTTACTACT CTCTTCCGAT GATGATGTCG CACTTATTCT 
   2301  ATGCTGTCTC AATGTTAGAG GCATATCAGT CTCCACTGAA GCCAATCTAT 
   2351  CTGTGACGGC ATCTTTATTC ACATTATCTT GTACAAATAA TCCTGTTAAC 
   2401  AATGCTTTTA TATCCTGTAA AGAATCCATT TTCAAAATCA TGTCAAGGTC 
   2451  TTCTCGAGGA AAAATCAGTA GAAATAGCTG TTCCAGTCTT TCTAGCCTTG 
   2501  ATTCCACTTC TGTCAGATGT GCCCTAGTCA GCGGAGACCT TTTGGTTTTG 
   2551  GGAGAGTAGC GACACTCCCA GTTGTTCTTC AGACACTTGG CGCACTTCGG 
   2601  TTTTTCTTTG GAGCACTTGA GCTTTTTAAG TCGGCAAATA TCGCATGCTT 
   2651  GTTCGATAGA AGACAGTAGC TTCATGGTGG CATCGCTAGC CACTAAGATC 
   2701  TGCATTTTGA CCTAGTTTAA AAGTAAAACA TAAGCGTGTC ACCTCAGGAT 
   2751  CATTGTATTT CTTGCTTTTA CACCTAATAT TCTTCAAAAT TAGCAACTAT 
   2801  TCCTTCAAAA TGCCTTAAAT ACGTTAATAT TCCGCAAGCC TCCCCTCAAT 
   2851  TTTACAAGGC TAAGTCACTT AATACGAAAT TCGTCATTCA AGGTTTCAAC 
   2901  GTCAACAGCG GAAAATCCAG AGCGCTCTCT TACCAAGCGT CCACAGCTTT 
   2951  CCCCACTAAC TGTTCCGGAT ATTCAAACTC CCCCGACCGA ACTCCAATCT 
   3001  GCCTCAAACC TAAGCACGGT GTTAGGCAAA CTGAAAACAA AACCAAACCC 
   3051  ACAAGACTCG CGAAATAAAA ATCCCATTGA AACACAAGAA GGGGCACCTT 
   3101  TCTCACACTG GGATTCCAAA GTAAAGGCTT CCAGAAACCA CACAAACTAC 
   3151  CTCTTTTCCG CCTTCCCCGC GAAGTAAACT CGACAGAATC GCCGATCGGT 
   3201  TAGGCGTCCG TTAGTTTCCG TCAACCCGCG CGCGCCAACC GCCCTCGCCC 
   3251  CCCTCAGGTC AACAAGGCCC ACTGTCCCGG AAAGGCCCAG CGCCGCGTCA 
   3301  CCTCGCCCTC CCCTCCCCCA CGCCCTCCCC TCAGCGGCCC ACCCAACCCC 
   3351  AAGAAGCCTC CACCTTGACC TCACCCATTC CCCCCTCTCA CACCCCCCCA 
   3401  AGGTCGTTAC GGCTGCGGGC CAGTACCTGT TAGCGGATAC CAGGATCCTG 
   3451  CCAATCACCA ACCACGTCCA CCCACAGGGA CACAAACAAG CTCACCCAAC 
   3501  AAAGCCAACC GCCCCTAAAT GCTCCGGGCT GGCGGAGGCA AATTTATGCA 
   3551  ACGAGTGTTG CGTCACTTAT CACCCCTCAC GTAGACGCCG TCTCCGTGCG 
   3601  CCGCTGAAAG TAGTTCGCCG CGCCCAACCT CCCGAGAGGC AGTTTAGGCT 
   3651  TTTCAACTGA GCCCCAAATT CCTCATAGCC GTAAGAAAGG CTCCTAAAAA 
   3701  ATTATTTCCC TTCTCCTCTA TGCGCTTCTC TTCCTTCTTT AGGAAAATAT
   3751  AAGAAAATTA TCAGGCGGAT GAATTTGAGT TGAAGAAACC TTCCTCGAAA 
   3801  GCTCTGGAAA CTTCCTTGCA GCGCCGGCAC TGATTGGTTA GGCCTGCGAA 
   3851  GGCGAGGTCG GCGCTGATTG GTGGGGGACG CGGTGGCTCT GTTGTTGCTG 
   3901  GGCTCCACCT GGGGTGGCTT AGCGCCGAGG TTGCTAAGTA ACTGAGCGCG 
   3951  CGTCGCCGCA CAGGCGCGCG CGCCGTCCTG AAAGAGGGTA TTTCCGGCGG 
   4001  GAGAAGGAGG GGGGGTCTTT CCTTTTTTTT CTTGAGACGG ATTTCGCTCT 
   4051  TGTTTCCCAG GCTGGAGTGC AATGGCGCGA TCTCGGCTCA TCGCAACCTC 
   4101  CGCCTCCCGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC AAGTAGCTGG 
   4151  GATTACAGGC ATGCGCCACC ACGTCCGGCT AATTTTTTGT ATTTTTAGTA 
   4201  GAGACGGGGT TTCACCATGT TTGTCAGGCT GGTCTCGAAC TCCTGACCTC 
   4251  AGGTAATCCA CCCGCCTCGG CCTGCCAAAG TGGGCCGCCG CGCCTCAGAA 
   4301  GCGGGACGGC GTGGCACCCG ATATCGGGCG CGCCGGCCTC CGCGCCGGGT 
   4351  TTTGGCGCCT CCCGCGGGCG CCCCCCTCCT CACGGCGAGC GCTGCCACGT 
   4401  CAGACGAAGG GCGCAGGAGC GTCCTGATCC TTCCGCCCGG ACGCTCAGGA 
   4451  CAGCGGCCCG CTGCTCATAA GACTCGGCCT TAGAACCCCA GTATCAGCAG 
   4501  AAGGACATTT TAGGACGGGA CTTGGGTGAC TCTAGGGCAC TGGTTTTCTT 
   4551  TCCAGAGAGC GGAACAGGCG AGGAAAAGTA GTCCCTTCTC GGCGATTCTG 
   4601  CGGAGGGATC TCCGTGGGGC GGTGAACGCC GATGATTATA TAAGGACGCG 
   4651  CCGGGTGTGG CACAGCTAGT TCCGTCGCAG CCGGGATTTG GGTCGCGGTT 
   4701  CTTGTTTGTG GATCGCTGTG ATCGTCACTT GGTGAGTAGC GGGCTGCTGG 
   4751  GCTGGCCGGG GCTTTCGTGG CCGCCGGGCC GCTCGGTGGG ACGGAAGCGT 
   4801  GTGGAGAGAC CGCCAAGGGC TGTAGTCTGG GTCCGCGAGC AAGGTTGCCC 
   4851  TGAACTGGGG GTTGGGGGGA GCGCAGCAAA ATGGCGGCTG TTCCCGAGTC 
   4901  TTGAATGGAA GACGCTTGTG AGGCGGGCTG TGAGGTCGTT GAAACAAGGT 
   4951  GGGGGGCATG GTGGGCGGCA AGAACCCAAG GTCTTGAGGC CTTCGCTAAT 
   5001  GCGGGAAAGC TCTTATTCGG GTGAGATGGG CTGGGGCACC ATCTGGGGAC 
   5051  CCTGACGTGA AGTTTGTCAC TGACTGGAGA ACTCGGTTTG TCGTCTGTTG 
   5101  CGGGGGCGGC AGTTATGCGG TGCCGTTGGG CAGTGCACCC GTACCTTTGG 
   5151  GAGCGCGCGC CCTCGTCGTG TCGTGACGTC ACCCGTTCTG TTGGCTTATA 
   5201  ATGCAGGGTG GGGCCACCTG CCGGTAGGTG TGCGGTAGGC TTTTCTCCGT 
   5251  CGCAGGACGC AGGGTTCGGG CCTAGGGTAG GCTCTCCTGA ATCGACAGGC 
   5301  GCCGGACCTC TGGTGAGGGG AGGGATAAGT GAGGCGTCAG TTTCTTTGGT 
   5351  CGGTTTTATG TACCTATCTT CTTAAGTAGC TGAAGCTCCG GTTTTGAACT 
   5401  ATGCGCTCGG GGTTGGCGAG TGTGTTTTGT GAAGTTTTTT AGGCACCTTT 
   5451  TGAAATGTAA TCATTTGGGT CAATATGTAA TTTTCAGTGT TAGACTAGTA 
   5501  AATTGTCCGC TAAATTCTGG CCGTTTTTGG CTTTTTTGTT AGACGAATCG 
   5551  CTAGCGCCGC CACCATGGGC CCTAAAAAGA AGCGTAAAGT CGCCCCCCCG 
   5601  ACCGATGTCA GCCTGGGGGA CGAGCTCCAC TTAGACGGCG AGGACGTGGC 
   5651  GATGGCGCAT GCCGACGCGC TAGACGATTT CGATCTGGAC ATGTTGGGGG 
   5701  ACGGGGATTC CCCGGGGCCG GGATTTACCC CCCACGACTC CGCCCCCTAC 
   5751  GGCGCTCTGG ATATGGCCGA CTTCGAGTTT GAGCAGATGT TTACCGATGC 
   5801  CCTTGGAATT GACGAGTACG GTGGGGAATT CGAGATGCCT GTGGACAGGA 
   5851  TCCTGGAGGC AGAGCTTGCT GTGGAACAGA AGAGTGACCA GGGCGTTGAG 
   5901  GGTCCTGGGG GAACCGGGGG TAGCGGCAGC AGCCCAAATG ACCCTGTGAC 
   5951  TAACATCTGT CAGGCAGCTG ACAAACAGCT ATTCACGCTT GTTGAGTGGG 
   6001  CGAAGAGGAT CCCACACTTT TCCTCCTTGC CTCTGGATGA TCAGGTCATA 
   6051  TTGCTGCGGG CAGGCTGGAA TGAACTCCTC ATTGCCTCCT TTTCACACCG 
   6101  ATCCATTGAT GTTCGAGATG GCATCCTCCT TGCCACAGGT CTTCACGTGC 
   6151  ACCGCAACTC AGCCCATTCA GCAGGAGTAG GAGCCATCTT TGATCGGGTG 
   6201  CTGACAGAGC TAGTGTCCAA AATGCGTGAC ATGAGGATGG ACAAGACAGA 
   6251  GCTTGGCTGC CTGAGGGCAA TCATTCTGTT TAATCCAGAG GTGAGGGGTT 
   6301  TGAAATCCGC CCAGGAAGTT GAACTTCTAC GTGAAAAAGT ATATGCCGCT 
   6351  TTGGAAGAAT ATACTAGAAC AACACATCCC GATGAACCAG GAAGATTTGC 
   6401  AAAACTTTTG CTTCGTCTGC CTTCTTTACG TTCCATAGGC CTTAAGTGTT 
   6451  TGGAGCATTT GTTTTTCTTT CGCCTTATTG GAGATGTTCC AATTGATACG 
   6501  TTCCTGATGG AGATGCTTGA ATCACCTTCT GATTCATAAT CTAGACGAGC 
   6551  AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC 
   6601  AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT 
   6651  GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA 
   6701  TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TAAAGCAAGT 
   6751  AAAACCTCTA CAAATGTGGT AAAATCCGAT AAGCGTACCT TCTGAGGCGG 
   6801  AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 
   6851  GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
   6901  ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 
   6951  CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 
   7001  TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
   7051  CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT 
   7101  ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 
   7151  AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGGCT AGAGCCACCA 
   7201  TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
   7251  AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC 
   7301  CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 
   7351  CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
   7401  TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT 
   7451  CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG 
   7501  ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
   7551  GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA 
   7601  CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 
   7651  GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
   7701  GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT 
   7751  CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG 
   7801  GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 
   7851  TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
   7901  CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT 
   7951  CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
   8001  CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA 
   8051  TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT 
   8101  TGTGTGAACG TACGAGTCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT 
   8151  TCCCTTTAGT GAGGGTTAAT TTCGAGCTTG GCGTAATCAT GGTCATAGCT 
   8201  GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
   8251  CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 
   8301  ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 
   8351  GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
   8401  GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 
   8451  GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
   8501  ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
   8551  AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 
   8601  AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
   8651  GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
   8701  GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 
   8751  TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
   8801  TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
   8851  ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 
   8901  CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
   8951  TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
   9001  TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 
   9051  TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
   9101  ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 
   9151  AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 
   9201  TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
   9251  GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
   9301  AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 
   9351  AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 
   9401  TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
   9451  GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 
   9501  CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 
   9551  CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
   9601  ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 
   9651  AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 
   9701  TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
   9751  CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 
   9801  GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 
   9851  GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
   9901  GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 
   9951  TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 
  10001  TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
  10051  GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 
  10101  AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 
  10151  AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
  10201  GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
  10251  GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 
  10301  ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGC