pLysE
At a Glance
pLysE Image made with PlasMapper
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
p15A |
| Copy # | |
| Markers |
Chloramphenicol |
| Link to Sequence | |
| Features |
T7 lysozyme coding seq. (pLysS) |
| Accession Number |
|
| edit table |
</protect>
Notes
This plasmid is not a cloning vector; it is used in lDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase.
History
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Sources
References
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Sequence
1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG
51 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA
101 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC
151 CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC
201 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT
251 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC
351 AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG
401 TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC
451 TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG
501 GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT
551 GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG
651 CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA
701 TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG
751 CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC
801 CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT
851 CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCTG
951 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC
1001 ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT
1051 CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT
1101 CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
1151 GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA
1201 GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC
1251 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT
1301 TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG
1351 TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA
1401 TCTTATTAAT CAGATAAAAT ATTTCTAGAT TTCAGTGCAA TTTATCTCTT
1451 CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT GTAATTCTCA
1501 TGTTTGACAG CTTATCATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT
1551 TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC
1601 ATCGTCATCC TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT
1651 TATGCCGGTA CTGCCGGGCC TCTTGCGGGA TATCGTCCAT TCCGACAGCA
1701 TCGCCAGTCA CTATGGCGTG CTGCTAGCGC TATATGCGTT GATGCAATTT
1751 CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG GCCGCCGCCC
1801 AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG
1851 CGACCACACC CGTCCTGTGG ATCCGGGTCC CCTTTGATAG ATTAAAAAGG
1901 AAAGGAGGAA AGAAATAATG GCTCGTGTAC AGTTTAAACA ACGTGAATCT
1951 ACTGACGCAA TCTTTGTTCA CTGCTCGGCT ACCAAGCCAA GTCAGAATGT
2001 TGGTGTCCGT GAGATTCGCC AGTGGCACAA AGAGCAGGGT TGGCTCGATG
2051 TGGGATACCA CTTTATCATC AAGCGAGACG GTACTGTGGA GGCAGGACGA
2101 GATGAGATGG CTGTAGGCTC TCACGCTAAG GGTTACAACC ACAACTCTAT
2151 CGGCGTCTGC CTTGTTGGTG GTATCGACGA TAAAGGTAAG TTCGACGCTA
2201 ACTTTACGCC AGCCCAAATG CAATCCCTTC GCTCACTGCT TGTCACACTG
2251 CTGGCTAAGT ACGAAGGCGC TGGTCTTCGC GCCCATCATG AGGTGGCGCC
2301 GAAGGCTTGC CCTTCGTTCG ACCTTAAGCG TTGGTGGGAG AAGAACGAAC
2351 TGGTCACTTC TGACCGTGGA TAATTAATTG AACTCACTAA AGGGAGACCA
2401 CAGCGGTTTC CCTTTGTTCG CATTGGAGGT CAAATAATGC GCAAGTCTTA
2451 TAAACAATTC TATAAGGCTC CGAGGAGGCA TATCCAAGTG TGGGAGGCAG
2501 CCAATGGGCC GGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG
2551 CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG
2601 AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT
2651 ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA
2701 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG
2751 GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG ACCGATGCCC
2801 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
2851 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
2901 AGGTGCCGGC AGCGCTCTGG GTCATTTTCG GCGAGGACCG CTTTCGCTGG
2951 AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA TCTTGCACGC
3001 CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA
3051 AGCAGGCCAT TATCGCCGGC ATGGCGGCCG ACGCGCTGGG CTACGTCTTG
3101 CTGGCGTTCG CGACGCGAGG CTGGATGGCC TTCCCCATTA TGATTCTTCT
3151 CGCTTCCGGC GGCATCGGGA TGCCCGCGTT GCAGGCCATG CTGTCCAGGC
3201 AGGTAGATGA CGACCATCAG GGACAGCTTC AAGGATCGCT CGCGGCTCTT
3251 ACCAGCCTAA CTTCGATCAC TGGACCGCTG ATCGTCACGG CGATTTATGC
3301 CGCCTCGGCG AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC
3351 TATACCTTGT CTGCCTCCCC GCGTTGCGTC GCGGTGCATG GAGCCGGGCC
3401 ACCTCGACCT GAATGGAAGC CGGCGGCACC TCGCTAACGG ATTCACCACT
3451 CCAAGAATTG GAGCCAATCA ATTCTTGCGG AGAACTGTGA ATGCGCAAAC
3501 CAACCCTTGG CAGAACATAT CCATCGCGTC CGCCATCTCC AGCAGCCGCA
3551 CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT GCGCATGATC
3601 GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG
3651 TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT
3701 GCAAAACGTC TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT
3751 GTTTCGTAAA GTCTGGAAAC GCGGAAGTCC CCTACGTGCT GCTGAAGTTG
3801 CCCGCAACAG AGAGTGGAAC CAACCGGTGA TACCACGATA CTATGACTGA
3851 GAGTCAACGC CATGAGCGGC CTCATTTCTT ATTCTGAGTT ACAACAGTCC
3901 GCACCGCTGT CCGGTAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
3951 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC
4001 CGGCAGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG
4051 CCGAAACAAG CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG
4101 CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTAAC
4151 CGTTTTTATC AGGCTCTGGG AGGCAGAATA AATGATCATA TCGTCAATTA
4201 TTACCTCCAC GGGGAGAGCC TGAGCAAACT GGCCTCAGGC ATTTGAGAAG
4251 CACACGGTCA CACTGCTTCC GGTAGTCAAT AAACCGGTAA ACCAGCAATA
4301 GACATAAGCG GCTATTTAAC GACCCTGCCC TGAACCGACG ACCGGGTCGA
4351 ATTTGCTTTC GAATTTCTGC CATTCATCCG CTTATTATCA CTTATTCAGG
4401 CGTAGCACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG
4451 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG
4501 CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG
4551 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG
4601 GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA
4651 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT
4701 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA
4751 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA
4801 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC
4851 CCATATCACC AGCTCACCGT CTTTCATTGC CATACG
